Mindan Tong
Yuhui Fu
Fang Chen
Shen Zhang
Hanmo Chen
Xi Ma
Defa Li
Xiaoxia Liu
Qing Zhong
1 State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2 Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
Funds: The work was supported by grants from NSFC (91754205, 91957204, 31771523, 31870830 and 31801170), MOST (2019YFA0508602), Shanghai Municipal Science and Technology Project (20JC1411100), Program of Shanghai Academic/Technology Research Leader (19XD1402200) and innovative research team of high-level local universities in Shanghai.
Received Date: 2020-07-02
Rev Recd Date:2020-08-07
Publish Date:2021-07-08
Abstract
Abstract
Autophagy is essential for the maintenance of cellular homeostasis and its dysfunction has been linked to various diseases. Autophagy is a membrane driven process and tightly regulated by membrane-associated proteins. Here, we summarized membrane lipid composition, and membrane-associated proteins relevant to autophagy from a spatiotemporal perspective. In particular, we focused on three important membrane remodeling processes in autophagy, lipid transfer for phagophore elongation, membrane scission for phagophore closure, and autophagosome-lysosome membrane fusion. We discussed the significance of the discoveries in this field and possible avenues to follow for future studies. Finally, we summarized the membrane-associated biochemical techniques and assays used to study membrane properties, with a discussion of their applications in autophagy.Keywords: autophagy,
membrane-associated proteins,
membrane-associated biochemistry assays,
ATG2,
ESCRT,
lipid transfer,
elongation,
scission,
fusion
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