Wenchen Shen
Bo Zhang
Kuan Tian
Yamu Li
Lili Mu
Zhiyuan Luo
Xiaoling Zhong
Xudong Wu
Ying Liu
Yan Zhou
1 College of Life Sciences, Renmin Hospital of Wuhan University, Medical Research Institute at School of Medicine, Wuhan University, Wuhan 430072, China;
2 Department of Cell Biology, Tianjin Medical University, Qixiangtai Road 22, Tianjin 300070, China
Funds: This work was supported by grants from National Key R&D Program of China (2018YFA0800700), National Natural Science Foundation of China (Grant Nos. 31671418 and 31471361), National Natural Science Foundation of Hubei Province (2018CFA016), Fundamental Research Funds for the Central Universities (2042017kf0242) and Wuhan University Experiment Technology Project Funding (WHU-2018-SYJS-01).
Received Date: 2019-06-11
Abstract
Abstract
The mechanisms underlying spatial and temporal control of cortical neurogenesis of the brain are largely elusive. Long non-coding RNAs (lncRNAs) have emerged as essential cell fate regulators. Here we found LncKdm2b (also known as Kancr), a lncRNA divergently transcribed from a bidirectional promoter of Kdm2b, is transiently expressed during early differentiation of cortical projection neurons. Interestingly, Kdm2b's transcription is positively regulated in cis by LncKdm2b, which has intrinsic-activating function and facilitates a permissive chromatin environment at the Kdm2b's promoter by associating with hnRNPAB. Lineage tracing experiments and phenotypic analyses indicated LncKdm2b and Kdm2b are crucial in proper differentiation and migration of cortical projection neurons. These observations unveiled a lncRNA-dependent machinery in regulating cortical neuronal differentiation.Keywords: long non-coding RNA,
neuronal differentiation,
cerebral cortex,
KDM2B,
divergent lncRNA
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