Multiple sgRNAs facilitate base editingmediated i-stop to induce complete and precise gene disruptio

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Kun Jia1,
Zongyang Lu3,
Fei Zhou2,
Zhiqi Xiong3,
Rui Zhang1,
Zhiwei Liu2,
Yu'e Ma2,
Lei He2,
Cong Li2,
Zhen Zhu4,
Dejing Pan2,,,
Zhengxing Lian1,,
1 Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing 100094, China;
2 Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University, Suzhou 215123, China;
3 School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China;
4 Key Laboratory of MEMS of Ministry of Education, Southeast University, Nanjing 210096, China
Funds: This work was supported by National Center for International Research (2017B01012), National Natural Science Foundation of China (Grant Nos. 31771299, 81600380 and 31600958), Natural Science Foundation of Jiangsu Province (BK20160313, BK20160317).

Abstract

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