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5' capped and 3' polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system

本站小编 Free考研考试/2022-01-02
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Wei Mu1,2,
Yongping Zhang3,
Xutong Xue4,
Lei Liu1,2,
Xiaofei Wei5,
Haoyi Wang1,
1 State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Department of Hematology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University, Beijing 100049, China;
4 Hebei University, Baoding 071002, China;
5 Beijing Cord Blood Bank, Beijing 100044, China
Funds: This work was supported by the National Natural Science Foundation of China (Grant No. 31471215), Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA16010205), National Key Research and Development Program of China (No. 2016YFA0101402), and National High-tech R&D Program (863 Program) (No. 2015AA020307).






Abstract



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http://www.protein-cell.org/article/exportPdf?id=a9166be3-ef56-44d9-af60-9eb3eab6c1a5&language=en
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