王孔江副研究员
kongjiangWang,AssociateProf.

专家类别:副研究员
简历&研究组工作摘要
1992.09-1995.07北京大学技术物理系放射化学专业,获理学博士1995.08-1997.07中国科学院高能物理研究所核技术开放实验室，博士后1997.08-1998.12中国科学院高能物理研究所核技术开放实验室，助研1999.01-2002.06美国RensselaerPolytechnicInstitute化学系　　　　　　　　Prof.JamesP.Ferris实验室（NSCORT）　　　　　　　　Troy,NY12170,NewYork,USA，博士后2002年7月-今　　中国科学院生物物理研究所，课题组长（技术创新PI）主要研究方向:蛋白质化学；生命起源问题；应用生物技术（ProteinChemistry;Originsoflife;Biotech.Innovation）1.与生命起源相关的化学问题研究在模拟生命起源过程相关的光化学反应过程中，发现了UV光激发磷酸根自由基向核酸单体的能量传递及光解促进效应（Wang*KJ,PanXM.,WuJL&WangWQ.,Photochem.Photobiol.,65:656-659,1997;Wang*KJ,ChaiZF&PanXM.,OriginsLifeEvol.Biosph.29:261-272,1999）。在进行手性起源问题的过程中，发现了Na+及Cl-等常见离子对带相反电荷的氨基酸聚合反应的高效催化效应（JieRen,LiangXin,Yi-NanLiu,Kong-JiangWang*,Macromolecules,41(6):1996-2002,2008;XinL.;MaYL.;LiuYN.,YanQ.;Wang*KJ,Biopolymers81:1-7,2006;Wang*KJ,3：YaoN.,LiC.,OriginsLifeEvol.Biosph.,2005,35:313-322;LiuY.N.Wang*K.J.,OriginsLifeEvol.Biosph.,2006,36:321-322;王孔江*,辛亮,任洁,化学进展,18(10):1391-1396,2006;Wang*KJ,RenJ.,XinL.,in“ProgressinBiopolymerResearch”,ed.,byPabloC.Sanchez(UniversityofMichigan,AnnArbor,Michigan,USA),2008,pp.3-7)。发现了光诱导的核酸还原反应（Wang*KJ&ChaiZF.,Chem.Commun.,1543-1544,1998.）；发现了精氨酸聚合反应过程中的一种新的中间产物并提出了新的聚合反应机理（LiangXin,Jun-FengXiang,JieRen,Kong-JiangWang*,Sci.ChinaSer.B-Chem.,52(8):1220-1226,2009）。建立了对多聚谷氨酸肽及多聚精氨酸肽等的高效HPLC分析方法（Wang*KJ&NaiP.,LiSS.,Anal.Biochem.,332:199-201,2004.)；并建立了核酸光解水化物的定量检测方法（Wang*KJ&ChaiZF.,J.Photochem.Photobiol.,A:Chemistry109:143-146,1997.)在JamesP.Ferris实验室进行博士后期间，参与了矿物RNA合成的催化效应的研究(WangKJ,Ferris*JP.,OriginsLifeEvol.Biosph.,2005,35:187-212；2:Ferris*JP,JoshiPC,WangKJ,MiyakawaS,HuangW.,Adv.SpaceRes.,33:100-105,2004.)目前正在进行与手性起源相关的研究。2．应用技术研究课题组近年来的研究转向与蛋白质相关的应用技术研究，主要通过为企业进行技术服务解决企业的关键技术问题。另外，课题组也瞄准一些与国民经济相关的关键技术问题进行技术研发。技术研发领域主要集中在：1）氨基酸原料药生产技术2）蛋白质水解技术3）生物制药技术（血液制品，胎盘制品）4）生物多肽药物标准的建立5）植物有效成分的提取及纯化技术近期主要研究项目：1)国家973项目(973-2007CB935901)；参与，2007-2010。2)中国科学院知识创新工程国防科技创新重大/重要方向项目；项目负责人，2009-2011。3)卫生部重大新药研制计划(2009ZX09103)；子项目负责人，2009-2012。4)国家科技支撑计划课题(公安部遏制毒品违法犯罪关键技术研究）；子项目负责人，2011-2013。近期主要企业项目：1）《新技术生产胱氨酸》，项目负责人。2）《有机微量元素生产技术》，项目负责人。3）《提高乙肝表面抗原抗体效价的实验室研究》；项目负责人。4）《利用大蒜加工副产物生产大蒜素》；项目负责人。5）《胎盘多肽质量标准及产品研发》；项目负责人。社会任职：中国远大集团成都蜀阳药业公司，技术顾问中国远大集团武汉远大弘元股份有限公司，技术顾问贵州泰邦生物科技有限公司，技术顾问北京市生物化学及分子生物学学会，理事

Biography&Introduction1992.09-1995.07TechnicalPhysicsDepartment,RadiationChem.,PekingUniversity,PH,D1995.08-1997.07NuclearAnalysisDepartment,InstituteofHighEnergyPhysics,ChineseAcademyofSciences,Postdoc.fellow1997.08-1998.12　NuclearAnalysisDepartment,InstituteofHighEnergyPhysics,ChineseAcademyofSciences,AssistantProf.1999.01-2002.06　DepartmentofChemistry,NSCORT,RensselaerPolytechnicInstitute,Troy,NY12170,NewYork,USA,Postdoc.fellow2002.07-Now　InstituteofBiophysics,ChineseAcademyofSciences,Prof.,PI(Biotech.)Thisgroupisinterestedin：1.TopicsrelatedtotheoriginsoflifeInoursimulationofthephotochemicalprocessesrelatedtotheoriginsoflife,wefoundtheenergytransferfromUV-excitedphosphateradicalstonucleicacidmonomersandtheenhancedphotolysisofnucleicacidmonomers（Wang*KJ,PanXM.,WuJL&WangWQ.,Photochem.Photobiol.,65:656-659,1997;Wang*KJ,ChaiZF&PanXM.,OriginsLifeEvol.Biosph.29:261-272,1999）。Thisisanadditionalpathwayofphotolysisofnucleicacids.Inourprobetotheoriginofbio-chirality,wefoundthehighlyefficientlongerpeptideformationenhancedbythemostcommonsimpleionslikeNa+andCl-（JieRen,LiangXin,Yi-NanLiu,Kong-JiangWang*,Macromolecules,41(6):1996-2002,2008;XinL.;MaYL.;LiuYN.,YanQ.;Wang*KJ,Biopolymers81:1-7,2006;Wang*KJ,3：YaoN.,LiC.,OriginsLifeEvol.Biosph.,2005,35:313-322;LiuY.N.Wang*K.J.,OriginsLifeEvol.Biosph.,2006,36:321-322）.Forreviews,pleaserefertofollowingpapers(王孔江*,辛亮,任洁,化学进展,18(10):1391-1396,2006;Wang*KJ,RenJ.,XinL.,in“ProgressinBiopolymerResearch”,ed.,byPabloC.Sanchez(UniversityofMichigan,AnnArbor,Michigan,USA),2008,pp.3-7)。Wealsoreported:1)Thephotoinducedreductionofthymineanduracilderivativesbyhypophosphite（Wang*KJ&ChaiZF.,Chem.Commun.,1543-1544,1998.）；2)Annewintermediateinthepolymerizationofarginineandtheproposedmechanismofargininepolymerization(LiangXin,Jun-FengXiang,JieRen,Kong-JiangWang*,Sci.ChinaSer.B-Chem.,52(8):1220-1226,2009).ThemethodsforboththeHPLCanalysisofoligopeptidesofL-glutamicacidandL-arginine（Wang*KJ&NaiP.,LiSS.,Anal.Biochem.,332:199-201,2004.)andtheaccuratedeterminationofdehydrationmethodsofuracilhydratederivatives（Wang*KJ&ChaiZF.,J.Photochem.Photobiol.,A:Chemistry109:143-146,1997.)arealsoestablished.DuringmypostdoctorresearchinProf.JamesP.Ferrislaboratory(NSCORT),Igotinvolvedinthemontmorillnite-catalyzedRNAsynthesis(WangKJ,Ferris*JP.,OriginsLifeEvol.Biosph.,2005,35:187-212；Ferris*JP,JoshiPC,WangKJ,MiyakawaS,HuangW.,Adv.SpaceRes.,33:100-105,2004.)2．Biotech.innovationThisgroupturnedtobiotechnicalinnovationsrelatedtoaminoacids,peptidesandproteinsinrecentyears.Thefollowingcriticaltechnicalproblemsareinvestigated:1）Aminoacidproduction2）Proteinhydrolysistech.3）Biopharmaceuticaltechniques

SelectedPublications：PapersasFirstauthorandCorrespondingauthor1）LiangXin,Jun-FengXiang,JieRen,Kong-JiangWang*,6-MemberedRingIntermediatesinPolymerizationofN-Carboxyanhydride-L-α-ArginineinH2O,Sci.ChinaSer.B-Chem.,52(8):1220-1226,2009.2）JieRen,LiangXin,Yi-NanLiu,Kong-JiangWang*,CopolymerizationofMixedL-α-argininewithL-α-glutamicAcid，Macromolecules,41(6):1996-2002,2008.3）Wang*KJ,RenJ.,XinL.,Na+andCl-enhancedpolymerizationoftheopposite-chargedaminoacids，in“ProgressinBiopolymerResearch”,ed.,byPabloC.Sanchez(UniversityofMichigan,AnnArbor,Michigan,USA),2008,pp.3-7.4）XinL.;MaYL.;LiuYN.,YanQ.;Wang*KJ,SodiumChlorideEnhancedOligomerizationofL-arginine,Biopolymers81:1-7,2006.5）王孔江*,辛亮,任洁,化学进展,18(10):1391-1396,2006.6）YanQ.,LiuY.N.Wang*K.J.,NaClenhancedoligomerizationofO-phospho-L-serine,OriginsLifeEvol.Biosph.,2006,36:321-322.7）Wang*KJ,YaoN.,LiC.,NaClenhancedoligomerizationofL-glutamicacidinaqueoussolution,OriginsLifeEvol.Biosph.,2005,35:313-322.8）Wang*KJ&NaiP.,LiSS.,Highresolutionhighperformanceliquidchromatographyseparationofpolyglutamicacids,Anal.Biochem.,332:199-201,2004.9）Wang*KJ&ChaiZF.,Photoinducedreductionofthymineanduracilderivativesbyhypophosphite:unusualhighquantumyieldofchromophoreloss.Chem.Commun.,1543-1544,1998.10）Wang*KJ,PanXM.,WuJL&WangWQ.,Enhancementofthephotolysisofnucleicacidmonomersbyphosphates,Photochem.Photobiol.,65:656-659,1997.11）Wang*KJ,ChaiZF&PanXM.,Enhancedphotolysisofnucleicacidmonomersbypyrophosphateinthesimulatedprimitivesoup,OriginsLifeEvol.Biosph.29:261-272,1999.12）Wang*KJ&ChaiZF.,Thecomparativeinvestigationofthedehydrationmethodsofuracilhydratederivatives,J.Photochem.Photobiol.,A:Chemistry109:143-146,1997.13）Wang*KJ.,Problemswithphosphatemethod,ISSOLNewsletter,25:21-22,1998.PapersasCo-author1)　WangKJ,Ferris*JP.,Catalysisandselectivityinprebioticsynthesis:initiationoftheformationofoligo(U)sonmontmorilloniteclaybyadenosine-5’-methylphosphate,OriginsLifeEvol.Biosph.,2005,35:187-212.2)　Ferris*JP,JoshiPC,WangKJ,MiyakawaS,HuangW.,Catalysisinprebioticchemistry:applicationtothesynthesisofRNAoligomers,Adv.SpaceRes.,33:100-105,2004.3)　PanXM,WangKJ,Wang*WQ,ShengXR,andWuJL.,Thechiralitybreakinginthephotolysisofleucineenantiomersand5’CMP,J.Biol.Physics,21:67-71,1995.4)　Liu*NQ,LiuP,WangK,ChenD,ZhaoJ,XuQ.“ASXRFmethodfordeterminingtherelativeconcentrationoftraceelementsinplasmaproteinaffectedbycisplatin”,BiolTraceElemRes.76:279-84,2000.5)　Wang*WQ,PanXMandWangKJ,Approachestotheoriginoflife,ProgressinNaturalSciences(Englishedition),4:708,1994.
联系方式(Contact)：


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