Arabidopsis O-GlcNAc transferase SEC activates histone methyltransferase ATX1 to regulate flowering
作 者：Xing LJ, Liu Y, Xu SJ, Xiao J, Wang B, Deng HW, Lu Z, Xu YY, Chong K*
影响因子:10.557
刊物名称:The EMBO Journal
出版年份:2018
卷:37期: 页码:e98115
文章摘要:Post-translational modification of proteins byO-linked β-N-acetylglucosamine (O-GlcNAc) is catalyzed byO-GlcNAc transferases (OGTs).O-GlcNAc modification of proteins regulates multiple important biological processes in metazoans. However, whether proteinO-GlcNAcylation is involved in epigenetic processes during plant development is largely unknown. Here, we show that loss of function ofSECRET AGENT(SEC), anOGTinArabidopsis, leads to an early flowering phenotype. This results from reduced histone H3 lysine 4 trimethylation (H3K4me3) ofFLOWERING LOCUSC(FLC) locus, which encodes a key negative regulator of flowering.SECactivates ARABIDOPSISHOMOLOG OF TRITHORAX1 (ATX1), a histone lysine methyltransferase (HKMT), throughO-GlcNAc modification to augmentATX1-mediated H3K4me3 histone modification atFLClocus.SECtransfers anO-GlcNAc group on Ser947 ofATX1,which resides in theSETdomain, thereby activatingATX1. Taken together, these results uncover a novel post-translationalO-GlcNAc modification-mediated mechanism for regulation ofHKMTactivity and establish the function ofO-GlcNAc signaling in epigenetic processes in plants.