简倩楠,
刘雪阳,
阮岑茜,
阿妮尔,
段安清,
王红芳,
郝晓冉,
北京师范大学生命科学学院，100875，北京
基金项目:国家自然科学基金资助项目（32071494）
详细信息
通讯作者:郝晓冉（1983-），女，博士，实验师. 研究方向: 分子真菌学. E-mail：2015xrhao@bnu.edu.cn
中图分类号:Q939.9计量
文章访问数:91
HTML全文浏览量:27
PDF下载量:10
被引次数:0
出版历程
收稿日期:2021-04-06
网络出版日期:2021-07-23
刊出日期:2021-09-02
Isolation of cellulolytic microorganisms from sika deer feces
Lan HUANG,Qiannan JIAN,
Xueyang LIU,
Cenqian RUAN,
Nier A,
Anqing DUAN,
Hongfang WANG,
Xiaoran HAO,
College of Life Sciences, Beijing Normal University, 100875, Beijing, China
摘要
HTML全文
图
参考文献
相关文章
施引文献
资源附件
访问统计
摘要
摘要:食草动物依赖胃肠道微生物分解利用天然木质纤维素物质。为获得高效纤维素降解菌，本文将东北梅花鹿粪便样品，经过研磨，重悬于羧甲基纤维素钠（CMC-Na）作为唯一碳源的富集培养基中，进行富集培养和筛选，用革兰氏碘液染色显示纤维素水解圈，通过纤维素水解圈直径（D）和菌落直径（d）比值，初步判断纤维素降解菌降解纤维素能力．筛选出106株纤维素降解微生物，包括72株细菌、22株真菌和12株放线菌．细菌16S rDNA和真菌ITS序列测序结果显示，这些纤维素降解菌主要集中分布于变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）、放线菌门（Actinobacteria）和子囊菌门（Ascomycota）．本文提供了一种快速有效的反刍动物肠道纤维素降解微生物的筛选方法，为东北梅花鹿肠道微生物高通量测序结果提供实验数据支持．
关键词:纤维素降解微生物/
肠道微生物/
东北梅花鹿/
反刍动物
Abstract:Herbivores rely on gastrointestinal microorganisms to decompose and utilize natural lignocellulosic materials．Here cellulose-degrading microorganisms were isolated from feces of sika deer (Cervus nippon hortulorum) to screen for highly efficient cellulose-degrading microorganisms．The feces samples were crushed, re-suspended in enrichment medium with sodium carboxymethyl cellulose (CMC-Na) as the sole carbon source．The sample suspension was diluted and spread on selection medium．Cellulose hydrolysis circle was measured by Gram’s iodine staining．Ratios of D (diameter of cellulose hydrolysis circle) to d (colony diameter) were used to determine cellulose degradation ability of the isolated microorganisms．Altogether 106 cellulose-degrading microorganisms were obtained, including 72 bacteria, 22 fungi and 12 actinomycetes．Bacterial 16S rDNA and fungal internal transcribed spacer (ITS) sequencing revealed that the isolated microorganisms were mainly distributed in Proteobacteria, Firmicutes, Actinobacteria and Ascomycota．
Key words:cellulose-degrading microorganisms/
gut microbiota/
Cervus nippon hortulorum/
ruminant animal
