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095200/480200兽医领域(专业学位)导师(4)
北京农学院 /2013-10-08
Northwest Agricultural and Forestall University
B.S.
M.S.
1987
1996
Veterinary Science
Veterinary Science
ACADEMIC APPOINTMENT
1987-1992: Lecturer in the Department of Animal Husbandry and Veterinary Science of Beijing Agricultural College.
1992-1997: Assistant Professor in the Department of Animal Husbandry and Veterinary Science of Beijing Agricultural College.
1999-2011: Associated Professor in the Department of Animal Science and Technology of Beijing Agricultural College.
2011.12- Professor in the College of Animal Science and Technology of Beijing University of Agriculture.
PROFESSIONAL AND RESEARCH EXPERIENCE
1985-1992: Focusing on teaching Poultry Science and Cattle Science in the Department of Animal Sciences in Beijing Agricultural College, and doing some research work on breeding of light layers, and on the application of some reproductive techniques (such as embryo transfer, embryo freezing, et al.) for sheep and dairy cattles.
1993-1997: Focusing on the nuclear transfer of bovine embryos, and production of transgenic rabbits and goats. Five transgenic rabbits (Human Insulin like Growth Factor-1, hIGF-1) and two transgenic goats (Bovine β-casein) were identified transgenic positively by Southern Blot Hybridization. Microinjection of foreign genes (such as bovine chamosin and hIGF-1) into bovine IVF (in vitro fertilization) embryos was also conducted. Construction of recombinant plasmids for gene transfer and positive transgenic animals were detected by PCR and Southern Blot Hybridization.
1998-2012 Focusing on the mechanism of ovine and bovine oocyte maturation in vitro by researching on the effects of EGF, PG and FSH AND LH on the development of their pre-implantation embryos. In vitro production of transgenic cloning of beef cattles.
Grant Support
National Fund for Natural Sciences of China ( 31072185, PI ) 2011/01/01 – 2013/12/31
The goal of this project is to explore the mechanism of the regulation of differential embryonic expression of IFNτ on different types of bovine embryos, and their in vitro induction of ISG15 on their uterine epithelium cells cultured in vitro. [ $5,600,0]
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant (2009ZX08007-005B, PI ) 2009/01/01—2012/7/31
The goal of this project is to breed a new transgenic clonig beef cattle breed. [ $8,340,0]
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant ( 2011ZX08008-005-03, PI ) 2011/08/01—2012/12/31
The goal of this project is to breed a new transgenic anti-arthritis encephalitis ovine breed. [ $8,340,0]
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant ( 2008ZX08008-005-02, Co-investigator, PI: Liu Yun-hai )
The goal of this project is to breed a new transgenic anti-arthritis encephalitis ovine breed. [ $19,400,0]
2008/08/01
National Eleventh “5-year-plan” “863 High Tech Major Plan” of China---Sub-grant ( 2008AA1003, PI)
The goal of this project is to explore the mechanism of in vitro maturation for ovine oocytes . [ $4,500,0]
2009/01/01
PUBLICATIONS
1. Zhang Shaoyu, Liu Yunhai, Ni Hemin, Wang Hao, Gu Meichao, Yan Peng, Yang Fei,Guo Yong, Gene expression profile analysis of dormant mouse embryos preserved by controlled slow freezing, Acta Laboratorium Animals Scientia Sinica ,2012, 20(5): 15-19,
2. Chen Li-ping, Piao Xue-jiao, Luo Yong, Ni He-min, Liu Yun-hai, Guo Yong, Study on the in-vitro cultured methods for the uterine endometrical cells of dairy cattles and sheep, Chinese Agricultural Science Bulletin, 2012, 28(14): 99-104,
3. Li Kai#, Ni He-min#, Liu Yun-hai, Zhao Yan-wei, Qu Yang-yan, Li Xiao-yan, Guo Yong, LH plays a role to enhance the nuclear and cytoplasm synchronization for in vitro maturation of ovine oocytes, Scientia Agricultura Sinica,2011, 10(1):136-141;(#:co-first author, *: correspondence author, indexed by SCIE)
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