1993-1997: Focusing on the nuclear transfer of bovine embryos, and production of                    transgenic rabbits and goats. Five transgenic rabbits (Human Insulin like Growth Factor-1, hIGF-1) and two transgenic goats (Bovine β-casein) were identified transgenic positively by Southern Blot Hybridization. Microinjection of foreign genes (such as bovine chamosin and hIGF-1) into bovine IVF (in vitro fertilization) embryos was also conducted. Construction of recombinant plasmids for gene transfer and positive transgenic animals were detected by PCR and Southern Blot Hybridization.
 
1998-2002:     Focusing on the mechanism of murine oocyte maturation by researching on the effects of recombinant human follicle stimulating hormone, recombinant human luteinizing hormone and recombinant human chorionic gonadotropin on the development of murine pre-implantation embryos.
 
2003.1-2005.5: As a postdoctoral research fellow in the Division of Reproductive and Developmental Biology in the Department of Pediatrics of Vanderbilt University Medical Center, Nashville, TN (mentor: S.K. Dey). Focusing on the special field of preimplantation and implantation biology as following:
1). Roles of lipid mediators in ovulation, fertilization and implantation using mutant mice.
2). Ligand-receptor signaling with endocannabinoids in early embryo development and implantation
 
HONOURS
 
1997            “Jingjiu - He Houhua Teacher Award ” from Beijing Agricultural College
2006            As a fellow of the Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality( PHB or IHLB )in 2006( from 2006 to 2008 ).
 
Grant Support
National Fund for Natural Sciences of China ( 31272526, PI )           2013/01/01 – 2016/12/31
The goal of this project is to explore the mechanism of the regulation of IFNτ,ISG15 and Wnt7a on different pre-implantation bovine embryos. [ $13,500,0]
 
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant ( 2013ZX08008-005-03, PI )      2013/01/01—2013/12/31
The goal of this project is to breed a new transgenic anti-arthritis encephalitis ovine breed. [ $8,340,0]
 
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant ( 2011ZX08008-005-03, PI )    2011/08/01—2012/12/31
The goal of this project is to breed a new transgenic anti-arthritis encephalitis ovine breed. [ $8,340,0]
 
National Fund for Natural Sciences of China ( 30871836, PI )           2009/01/01 – 2011/12/31
The goal of this project is to explore the mechanism of the regulation of both IFNτand Galectin15 on early implantation of ovine IVF embryos.[ $4,500,0]
 
Municipal Key Fund for Natural Sciences of Beijing Municipal Government
The goal of this project is to explore the functional analysis of both mouse and bovine trophoderm stem cells. [ $2,990,0]
 
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant ( 2008ZX08008-005-02, Co-investigator, PI: Liu Yun-hai )
The goal of this project is to breed a new transgenic anti-arthritis encephalitis ovine breed. [ $19,400,0]
2008/08/01 – 2010/12/31
National Eleventh “5-year-plan” “863 High Tech Major Plan” of China---Sub-grant  ( 2008AA1003，Co-investigator, PI: Ni Hemin )
The goal of this project is to explore the mechanism of in vitro maturation for ovine oocytes . [ $4,500,0]
2009/01/01 – 2011/12/31
 
 
PUBLICATIONS
 
1. Zhang Shaoyu, Liu Yunhai, Ni Hemin, Wang Hao, Gu Meichao, Yan Peng, Yang Fei, Guo Yong*, Gene expression profile analysis of dormant mouse embryos preserved by controlled slow freezing, Acta Laboratorium Animals Scientia Sinica ,2012, 20(5): 15-19, (*: correspondence author)
2. Chen Li-ping, Piao Xue-jiao, Luo Yong, Ni He-min, Liu Yun-hai, Guo Yong*, Study on the in-vitro cultured methods for the uterine endometrical cells of dairy cattles and sheep, Chinese Agricultural Science Bulletin, 2012, 28(14): 99-104,(*: correspondence author)
3. Li Kai#, Ni He-min#, Liu Yun-hai, Zhao Yan-wei, Qu Yang-yan, Li Xiao-yan, Guo Yong*, LH plays a role to enhance the nuclear and cytoplasm synchronization for in vitro maturation of ovine oocytes, Scientia Agricultura Sinica,2011, 10（1）：136-141；(#：co-first author, *: correspondence author, indexed by SCIE)