Hepatocellular carcinoma (HCC) is highly heterogeneous in nature and has been one of the most common cancer types worldwide. To ensure repeatability of identified gene expression patterns and comprehensively annotate the transcriptomes of HCC, we carefully curated 15 public HCC expression datasets that cover around 4000 clinical samples and developed the database HCCDB to serve as a one-stop online resource for exploring HCC gene expression with user-friendly interfaces. The global differential gene expression landscape of HCC was established by analyzing the consistently differentially expressed genes across multiple datasets. Moreover, a 4D metric was proposed to fully characterize the expression pattern of each gene by integrating data from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx). To facilitate a comprehensive understanding of gene expression patterns in HCC, HCCDB also provides links to third-party databases on drug, proteomics, and literatures, and graphically displays the results from computational analyses, including differential expression analysis, tissue-specific and tumor-specific expression analysis, survival analysis, and co-expression analysis. HCCDB is freely accessible at http://lifeome.net/database/hccdb.
肝细胞癌是一种常见且死亡率很高的癌症。高通量生物技术的发展使得人们可以从不同的分子水平对HCC进行描述。目前，表达谱数据已经大量积累并广泛应用于分子分型和肿瘤标志物的研究中。然而，对于HCC这样一种高度异质的疾病，只从一个样本量有限的表达谱数据集中做出的推断，很容易出现可重复性差和假阳性高的问题。为了找到可重复性强的异常表达模式、全面注释HCC中的基因表达情况，我们收集了15个公共的表达谱数据集，总共近4,000个临床样本，并开发了数据库HCCDB，提供用户友好的一站式在线检索服务。我们通过分析多个数据集得到一致差异表达基因图谱，再结合TCGA和GTEx的数据定义了一个4D指标，用于全面描述每个基因的表达模式。此外，HCCDB还提供药物、蛋白、文献挖掘等第三方链接，并通过图表直观地展示差异分析、组织特异性、生存期分析以及共表达等计算结果。HCCDB数据库网址：http://lifeome.net/database/hccdb.





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