柳志强

工作部门:生物与环境工程学院\生物工程学科
性别:男
技术职称:高校教授
最高学位:博士
民族:汉族
籍贯:吉林榆树市

柳志强，博士，教授，美国纽约大学博士后。2005年毕业于江南大学发酵专业，获博士学位。2013年入选浙江省第一批青年科学家培养计划；2010年入选浙江省151人才（第二层次）计划、并获浙江省留学人员科技活动择优项目资助；入选2010年度省高校优秀青年教师资助计划，同年入选法国“科研创新人才”工程；2009年入选浙江省钱江人才（D类）计划，2007年、2009年先后被评为浙江工业大学青年学术骨干和青年学术带头人。主要从事微生物、酶工程、基因工程、生物催化与手性技术及酶分子改造等的教学和科研工作。主持国家“863”目标导向型项目、国家“863”重大项目子课题、浙江省自然科学基金杰出青年项目、浙江省科技厅重大项目、浙江省自然科学基金项目、浙江省科技厅面上项目各1项；作为技术骨干参加国家“973”项目2项、国家“863”重大项目2项、国家自然科学基金项目1项、浙江省自然基金项目5项、浙江省科技厅项目4项、参与浙江省重大横向项目8项。在国内外学术期刊发表学术论文60多篇，其中SCI收录50多篇。参与申请发明专利30多项，授权20项。联系方式：办公室电话：88320614E-mail：microliu@gmail.com1、主持的科研、人才项目：[8]浙江省科技厅重大专项项目，冬虫夏草百令胶囊大规模生产中现代生物技术方法的开发与应用，2012.09-2015-09[7]国家863目标导向型项目，生物催化外消旋扁桃腈生产R-扁桃酸的产业化技术研究与开发，2009.1-2011.12[6]国家863重点项目子课题，手性酸生产相关生物催化剂的改造，2011.1-2015.12[5]浙江省自然科学基金基杰出青年项目，腈水解酶序列、结构与功能关系及催化机理研究，2011.01-2013.12[4]浙江省留学人员科技活动择优资助项目（优秀类），2010[3]浙江省人力资源和社会保障厅钱江人才项目（D类），2010[2]浙江省自然科学基金基项目，酶的定向改造及双酶耦联生物法生产D-氨基酸的应用基础研究，2007.01-2009.12[1]浙江省科技厅面上项目，生物催化与转化2-氰基吡嗪制备抗结核病药物吡嗪酰胺，2007.01-2008.122、发表的文章：2013[52]ZhiqiangLiu,PeterJamesBaker,FengCheng,YapingXue,YuguoZheng,andYinchuShen.ScreeningandImprovingtheRecombinantNitrilasesandApplicationinBiotransformationofIminodiacetonitriletoIminodiaceticAcid.PLoSOne.2013,8(6):e67197.[51]ImprovementofnitrilaseproductionfromanewlyisolatedAlcaligenesfaecalismutantforbiotransformationofiminodiacetonitriletoiminodiaceticacid.JournaloftheTaiwanInstituteofChemicalEngineers.2013,2,169-176.[50]LiqunJin,ZhiqiangLiu,JianmiaoXu,YuguoZheng.Efficientbiocatalytichydrolysisof2-chloronicotinamideforproductionof2-chloronicotinicacidbyrecombinantamidase.CatalysisCommunications.2013,38,6-9.[49]LiqunJin,ZhiqiangLiu,JianmiaoXu,YuguoZheng.Biosynthesisofnicotinicacidfrom3-cyanopyridinebyanewlyisolatedFusariumproliferatumZJB-09150.WorldJournalofMicrobiologyandBiotechnology.2013,29,431-440.[48]ZhongyuYou,ZhiQiangLiu,YuGuoZheng,YinChuShen.Characterizationandapplicationofanewlysynthesized2-deoxyribose-5-phosphatealdolase.JournalofIndustrialMicrobiologyandBiotechnology.2013,40,29-39.[47]ZhongyuYou,ZhiQiangLiu,YuGuoZheng,Propertiesandbiotechnologicalapplicationsofhalohydrindehalogenases:currentstateandfutureperspectives.AppliedMicrobiologyandBiotechnology.2013,97,9-21[46]YaPingXue,MingXu,HongShengChen,WeiWang,ZhiqiangLiu,YaJunWang,YuguoZheng.ANovelIntegratedBioprocessforEfficientProductionof(R)-(-)-MandelicAcidwithImmobilizedAlcaligenesfaecalisZJUTB10.OrganicProcessResearch&Development.2013,17,213-220.2012[45]ZhiQiangLiu,MingZhou,XinhongZhang,JianMiaoXu,YuGuoZheng.BiosynthesisofIminodiaceticAcidfromIminodiacetonitrilebyImmobilizedRecombinantEscherichiacoliHarboringNitrilase.JournalofMolecularMicrobiologyandBiotechnology.2012,22,35-47.[44]ZhongCeHu,ZhiQiangLiu,JianMiaoXu,YuGuoZheng,YinChuShen.Improvementof1,3-dihydroxyacetoneproductionfromGluconobacteroxydansbyionbeamimplantation.PreparativeBiochemistryandBiotechnology.2012,42,15-28.[43]ZhiQiangLiu,XiaoBoZheng,SuPingZhang,YuGuoZheng.Cloning,expressionandcharacterizationofalipasegenefromtheCandidaantarcticaZJB09193anditsapplicationinbiosynthesisofvitaminAesters.MicrobiologicalResearch.2012,167,452-460.[42]JinFengZhang,ZhiQiangLiu,YuGuoZheng,YinChuShen.Screeningandcharacterizationofmicroorganismscapableofconvertingiminodiacetonitriletoiminodiaceticacid.EngineeringinLifeSciences.2012,12,69-78.[41]YaJunWang,ZhiQiangLiu,RenChaoZheng,YaPingXue,YuGuoZheng.Screening,cultivation,andbiocatalyticperformanceofRhodococcusboritoleransFW815withstrong2,2-dimethylcyclopropanecarbonitrilehydrataseactivity.JournalofIndustrialMicrobiologyandBiotechnology.2012,39,409-417.[40]HuoXiJin,ZhongCeHu,ZhiQiangLiu,YuGuoZheng.Nitrite-mediatedsynthesisofchiralepichlorohydrinusinghalohydrindehalogenasefromAgrobacteriumradiobacterAD1.BiotechnologyandAppliedBiochemistry.2012,59,170-177.[39]ZhiQiangLiu,LingZhang,LiHuiSun,XiaoJunLi,NanWeiWan,YuGuoZheng.Enzymaticproductionof5‘-inosinicacidbyanewlysynthesisedacidphosphatase/phosphotransferase.FoodChemistry.2012,134,948-956.[38]YaPingXue,WeiWang,YaJunWang,ZhiqiangLiu,YuguoZheng,YinchuShen.Isolationofenantioselectivealpha-hydroxyaciddehydrogenasesbasedonahigh-throughputscreeningmethod.BioprocessandBiosystemsEngineering.2012,35,1515-1522.[37]PeterJamesBaker,ChristopherPoultney,ZhiqiangLiu,RichardGross,JinKimMontclare.Identificationandcomparisonofcutinasesforsyntheticpolyesterdegradation.AppliedMicrobiologyandBiotechnology,2012,93,229-240.2011[36]ZhiqiangLiu,LizhuDong,FengCheng,YapingXue,YuguoZheng,YinchuShen.Genecloning,over-expressionandcharacterizationofanitrilasefromAlcaligenesfaecailsZJUTB10.JournalofAgricultureandFoodChemistry.2011,59,11560-11570.[35]ZhiqiangLiu,LipingZhang,ChengFeng,LitaoRuan,ZhongceHu,YuguoZheng,YinchuShen.Characterizationofanewlysynthesizedepoxidehydrolaseanditsapplicationinracemicresolutionof(R,S)-epichlorohydrin.CatalysisCommunications.2011,16,133-139.[34]ZhiqiangLiu,FeifeiLi,FengCheng,TaoZhang,ZhongyuYou,JianmiaoXu,YapingXue,YuguoZheng,YinchuShen.Anovelsynthesisofiminodiaceticacid:BiocatalysisbywholeAlcaligenesfaecalisZJB-09133cellsfromiminodiacetonitrile.BiotechnologyProgress.2011,27,698-705.[33]YajunWang,LilingLiu,ZhihuaFeng,ZhiqiangLiu,YuguoZheng.OptimizationofMediaCompositionandCultureConditionsforAcarboseProductionbyActinoplanesutahensisZJB-08196.WorldJournalofMicrobiologyandBiotechnology.2011,27,2759-2766.[32]YapingXue,XuSaizhen,ZhiqiangLiu,YuguoZheng,YinchuShen.Enantioselectivebiocatalytichydrolysisof(R,S)-mandelonitrileforproductionof(R)-(-)-mandelicacidbyanewlyisolatedAlcaligenesfaecalismutant.JournalofIndustrialMicrobiologyandBiotechnology.2011,38,337–345.[31]YapingXue,ZhiqiangLiu,YuguoZheng,YinchuShen.EfficientSeparationof(R)-(-)-mandelicacidbiosynthesizedfrom(R,S)-mandelonitrilewithnitrilasebyion-exchangeprocess.JournalofChemicalTechnologyandBiotechnology.2011,86,391–397.[30]HuapingDong,ZhiqiangLiu,YuguoZheng,YinchuShen.MediumoptimizationfornitrilaseproductionbyanewlyisolatedRhodococcuserythropolisZJB-0910usingstatisticaldesigns.ChemicalandBiochemicalEngineeringQuarterly.2011,5,351–358.[29]LiqunJin,YafeiLi,ZhiqiangLiu,YuguoZheng,YinchuShen.CharacterizationofanewlyisolatedstrainRhodococcuserythropolisZJB-09149transforming2-chloro-3-cyanopyridineto2-chloronicotinicacid.NewBiotechnology.2011,28,610-615.2010[28]ZhiqiangLiu,LizhuangJia,YuguoZheng.IsolationandcharacterizationofSerratiamarcescensZJB-07166,capableofconvertingDL-lactatetopyruvate.ProcessBiochemistry.2010,45,1632-1637.[27]YapingXue,ZhiqiangLiu,YuguoZheng,YinchuShen.Enhancedbiotransformationof(R,S)-mandelonitrileto(R)-(-)-mandelicacidwithinsituproductionremovalbyadditionofresin.BiochemicalEngineeringJournal.2010,53,143-149.[26]ZhongceHu,ZhiqiangLiuandYuguoZheng.Productionof1,3-dihydroxyacetonefromglycerolbyGluconobacteroxydansZJB09112.JournalofMicrobiologyandBiotechnology.2010,20,340-345.[25]LiqunJin,ZhiqiangLiu,YuguoZheng,YinchuShen.IdentificationandcharacterizationofSerratiamarcescensZJB-09104,anitrile-convertingbacterium.WorldJournalofMicrobiologyandBiotechnology.2010,26,817-823.[24]FengjieCui,ZhiqiangLiu,YinLi,DamingHuang.ApplicationofDoehlertdesignforoptimizingtheproductionofmycelialbiomassandexo-polymerbyHericiumerinaceusCZ-2.BiotechnologyandBioprocessEngineering.2010,15,299-307.[23]HuapingDong,ZhiqiangLiu,YuguoZheng,YinchuShen.(R)-enantioselectivehydrolysisofracemicethyl4-cyano-3-hydroxybutyatebywholecellsofanewlyisolatedstrainRhodococcuserythropolisZJB-0910.AppliedMicrobiologyandBiotechnology.2010,87,1335-1345.[22]YuanshanWang,RenchaoZheng,JianmiaoXu,ZhiqiangLiu,FengCheng,ZhihuaFeng,LilingLiu,YuguoZheng?,YinchuShen.Enantioselectivehydrolysisof(R)-2,2-dimethylcyclopropanecarboxamidebyimmobilizedcellsofanR-amidaseproducingbacteriumDelftiatsuruhatensisCCTCCM205114onalginatecapsulescarrier.JournalofIndustrialMicrobiologyandBiotechnology.2010,37,503-510.2009[21]ZhiqiangLiu,YuyingGosser,PeterJamesBaker,YanivRavee,ZiyingLu,GirumAlemu,HuiguangLi,GlennL.Butterfoss,Xiang-PengKong,RichardGross,andJinKimMontclare.StructuralandFunctionalStudiesofAspergillusoryzaeCutinase:EnhancedThermostabilityandHydrolyticActivityofSyntheticEsterandPolyesterDegradation.JournalofAmericanChemistrySociety.2009,131,15711-15716.[20]YinLi,ZhiqiangLiu,FengjieCui,ZhishengLiu.IsolationandidentificationofanewlyisolatedAlternariasp.ND-16andcharacterizationofxylanase.AppliedBiochemistryandBiotechnology.2009,57,36-49.[19]YinLi,FengjieCui,ZhiqiangLiu,ZhishengLiu,HuiZhao.OptimizationoffermentationconditionsforproductionofnovelxylanasebyPenicilliumthiersiiZH-19.WorldJournalofMicrobiologyandBiotechnology.2009,25,721–725.[18]ShenMei,YuguoZheng,ZhiqiangLiuandYinchuShen.ProductionofacrylicacidfromacrylonitrilebyimmobilizationofArthrobacternitroguajacolicusZJUTB06-99.JournalofMicrobiologyandBiotechnology.2009,19,582-587.[17]ShenMei,ZhiqiangLiu,YuguoZhengandYinchuShen.Enhancingendo-nitrilaseproductionbyanewlyisolatedArthrobacternitroguajacolicusZJUTB06-99throughoptimizationofculturemedium.BiotechnologyandBioprocessEngineering.2009,14,795-802.2008[16]ZhiqiangLiu,ZhongceHu,YuguoZhengandYinchuShen.Optimizationofcultivationconditionforproducingthe1,3-dihydroxyacetonefromnewlyisolatedPichiamembranifaciensusingresponsesurfacemethodology.BiochemicalEngineeringJournal.2008,38,285-291.[15]ZhiqiangLiu,JianfenZhang,YuguoZhengandYinchuShen.Productionofastaxanthinfromanewlyisolatedphaffiarhodozymamutantbylow-energyionsbeamimplantation.JournalofAppliedMicrobiology.2008,108,861-872.[14]ZhiqiangLiu,YinLi,LifengPing,FengjieCui,YuguoZheng.Optimizationofproductionandcharacterizationofenzyme-treatedoctenylsuccinicanhydridemodifiedwaxycornstarch.JournalofAgriculturalandFoodChemistry.2008,56,11499–11506.[13]YuguoZheng,JingChen,ZhiqiangLiu,MinghuoWu,LiangyingXingandYinchuShen.Isolation,identificationandcharacterizationofBacillussubtilisZJB-063,aversatilenitrile-convertingbacterium.AppliedMicrobiologyandBiotechnology.2008,77,985-993.2007[12]ZhiqiangLiu,YinLi,YingyingXu,LifengPingandYuguoZheng.Cloning,sequencing,andexpressionofanovelepoxidehydrolasegenefromRhodococcusopacusinEscherichiacoliandcharacterizationofenzyme.AppliedMicrobiologyandBiotechnology.2007,74,99-106.[11]ZhiqiangLiu,YinLi,LifengPing,YingyingXu,FengjieCuiandYuguoZheng.IsolationandidentificationofanovelRhodococcussp.ML-0004producingepoxidehydrolaseandoptimizationofenzymeproduction.ProcessBiochemistry.2007,42,889–894.[10]YinLi,ZhiqiangLiu,HuiZhao,YingyingXuandFengjieCui.StatisticaloptimizationofxylanaseproductionfromnewisolatedPenicilliumoxalicumZH-30insubmergedfermentation.BiochemicalEngineeringJournal.2007,34,82-86.[9]YinLi,ZhiqiangLiu,FengjieCui,YingyingXuandHuiZhao.ApplicationofstatisticalexperimentaldesigntooptimizeculturerequirementsofanovelAspergillussp.ZH-26producingendoxylanasefordegradationofarabinoxylansinmashing.JournalofFoodScience.2007,72,E320-E329.[8]YinLi,ZhiqiangLiu,FengjieCui,YingyingXuandHuiZhao.ProductionofxylanasefromanewlyisolatedPenicilliumsp.ZH-30.WorldJournalofMicrobiologyandBiotechnology.2007,23,837-843.[7]YinLi,ZhiqiangLiu,FengjieCuiandZhishengLiuandHuiZhao.ApplicationofPlackett-BurmanexperimentaldesignandDoehlertdesigntoevaluatenutritionalrequirementsforxylanaseproductionbyAlternariamaliND-16.AppliedMicrobiologyandBiotechnology.2007,77,285-291.[6]JianfenZhang,YuguoZheng,ZhiqiangLiuandYinchuShen.Preparationof3-ketovalidoxylamineAC-Nlyasesubstrate:N-p-nitrophenyl-3-ketovalidaminebyStenotrophomonasmaltrophiliaCCTCCM204024.AppliedMicrobiologyandBiotechnology.2007,73,1275-1281.[5]YinLi,FengjieCui,ZhiqiangLiu,YingyingXuandHuiZhao.ImprovementofxylanaseproductionbyPenicilliumoxalicumZH-30usingresponsesurfacemethodology.EnzymeandMicrobialTechnology.2007,40,1381-1388.[4]RenchaoZheng,YuanshanWang,ZhiqiangLiu,LiangyingXing,YuguoZhengandYinchuShen.IsolationandcharacterizationofDelftiatsuruhatensisZJB-05174,capableofR-enantioselectivedegradationof2,2-dimethylcyclopropanecarboxamide.ResearchinMicrobiology.2007,158,258-264.[3]Fengjiecui,YinLi,YingyingXu,ZhiqiangLiu,DamingHuang,ZhicaiZhangandWenyiTao.InductionofapoptosisinSGC-7901cellsbyPolysaccharide-peptideGFPS1bfromtheculturedmyceliaofGrifolafrondosaGF9801.ToxicologyinVitro.2007,21,417-427.2006[2]ZhiqiangLiu,ZhihaoSunandYongLeng.DirectedEvolutionofD-pantonohydrolasefromFusariummoniliforme.JournalofAgriculturalandFoodChemistry.2006,54,5823-5830.2004[1]ZhiqiangLiuandZhihaoSun.CloningandexpressionofD-lactonohydrolasecDNAfromFusariummoniliformeinSaccharomycescerevisiae.BiotechnologyLetters.2004,26,1861-1865.3、授权专利：[17]冬虫夏草中国被毛孢合成代谢甘露醇相关酶、基因及应用ZL201110267161.3[16]藤黄微球菌及其催化亚氨基二乙腈制备亚氨基二乙酸ZL201110223794.4[15]节杆菌及其催化亚氨基二乙腈制备亚氨基二乙酸ZL201110224295.7[14]一种环氧化物水解酶基因、载体、工程菌及其应用ZL201010226216.1[13]生物催化与分离耦合法生产R-扁桃酸ZL200910155228.7[12]生物催化法生产R-扁桃酸及其衍生物的方法ZL200910154484.4[11]一种甘油脱水酶基因.载体.工程菌及其应用ZL200910153309.3[10]一种水解酶基因、载体、工程菌及其应用ZL200910152434.2[9]微生物催化亚氨基二乙腈制备亚氨基二乙酸的方法ZL200910100875.8[8]亚氨基二乙酸的液相分析方法ZL200810122190.9[7]微生物催化法生产亚氨基二乙酸及其菌株ZL200810122191.3[6]生物催化2-氰基吡嗪酰胺的方法及其菌株ZL200810163750.5[5]地衣芽孢杆菌B-05571及其在制备1，3-二羟基丙酮中的应ZL200610053548.8[4]枯草芽孢杆菌ZJB-063及其应用ZL200610050594.2[3]赤红球菌ZJB-064及其应用ZL200610050593.8[2]微生物法生产二羟基丙酮ZL200510061969.0[1]D-泛解酸内酯水解酶cDNA的克隆、表达及其应用ZL200410041614.0