张文

　　工作部门:药学院化学生物学

　　性 别:男

　　技术职称:高校教授

　　最高学位:博士

　　民 族:汉族

　　籍 贯:黑龙江 大庆市

　　姓名: 张 文

　　性别: 男

　　职称/学位/职位: 教授/博士(工学)，博士生导师，‘十二五’浙江省高校“化学生物学”重点学科负责人

　　从事专业：化学生物学，生物有机化学，药物化学

　　Tel: (+86)-571-88871507；Fax:(+86)-571-88871507；13456941929；

　　E-mail: wzhang63@zjut.edu.cn

　　教育背景及工作经历：

　　1992年9月—1995年4月 大连理工大学 化工学院 应用化学专业 硕士研究生

　　研究方向: 有机合成及多相催化 导师：王大庆 教授，李同信 研究员

　　1998年9月—2001年11月 华东理工大学 化学与制药工程学院 应用化学专业 博士研究生

　　研究方向: 生物有机荧光染料与核酸化学 导师：钱旭红 教授

　　2001年12月—2003年11月 日本国 东北大学 生命科学研究科 分析生物有机化学研究室 日本文部省JSPS博士后研究员

　　研究领域: 生物有机化学　(生物有机荧光染料与核酸化学) 合作导师：大類 洋（Hiroshi Ohrui）教授

　　2003年12月—2006年3月 日本国 京都大学 理学研究科 化学系 化学生物研究室 日本文部省COE & JST博士后研究员

　　研究领域: 化学生物学 (寡肽聚酰胺与核酸化学) 合作导师：杉山 弘（Hiroshi Sugiyama）教授

　　2006年4月—2008年3月 日本国 Chemical Soft R & D Inc. 首席研究员

　　研究领域: 有机合成化学与生物有机化学(有机小分子与核酸化学)

　　2008年3月—至今 日本国 Chemical Soft R & D Inc. 客员首席研究员

　　2008年3月—至今 浙江工业大学 药学院 教授 研究领域: 化学生物学与分子药物设计（功能有机小分子及与核酸作用的化学与生物学研究）

　　Research Interest: Biology & Chemistry of Small Functional Molecules and Their Interaction with Nucleic Acids

　　(1) Interpretation of structural biology of interaction of small molecules with nucleic acids(Polyamide,Aminoglycoside,B-DNA,G-DNA,RNA)

　　(2) Gene regulation by small molecules and design of molecular targeted-drugs

　　(3) Design and synthesis of small bioactive molecules

　　(4) Study on DNA Nano Structure

　　Experience:

　　Mar.2008 to now

　　Professor, College of Pharmaceutical Sciences, Zhejiang University of Technology, China.

　　Apr.2006 to Feb.2008

　　Chief Executive Researcher, Mie R&D Center, Chemical Soft R&D Inc., Japan

　　Jan.2006 to Mar.2006

　　JST postdoctoral fellow, funded by Japan Science and Technology Agency.

　　Dec.2003 to Dec. 2005

　　COE postdoctoral fellow, funded by the Ministry of Education, Culture, Sports, Technology and Science of Japan.

　　Dec.2001 to Nov.2003

　　JSPS postdoctoral fellow, funded by Japan Society for the Promotion of Science, Japan.

　　Sept.1996 to Aug.1998

　　Associate professor of Chemistry, Department of Engineering, Daqing Broadcast & TV University, Daqing, China.

　　May 1995 to Aug.1996 & Sept.1990 to Aug.1992

　　Instructor, Department of Engineering, Daqing Broadcast & TV University, Daqing, China.

　　Aug.1987 to Aug.1990

　　Teaching assistant, Department of Engineering, Daqing Broadcast & TV University, Daqing, China.

　　Jul.1983 to Sept.1984

　　Chemistry teacher, Technology Education Division of Daqing Oilfield Administration Bureau, Daqing, Heilongjiang, China.

　　Education:

　　Sept. 1998 to Dec. 2001

　　Institute of Pesticides & Pharmaceuticals, East China University of Science and Technology, Shanghai, China.

　　Doctoral program in Applied Chemistry with Prof. Dr. Xuhong QIAN with specialization in Synthetic & Biological Organic Chemistry of naphthalene-contained heterocyclic compounds and the thesis entitled: “The Photochemistry and Photobiochemstry of Naphthalene-contained Heterocyclic Derivatives--Recognition, Labeling, Cleavage and Resolution for Bioorganic Molecules”.

　　Sept. 1992 to Apr. 1995

　　School of Graduate, Dalian University of Technology, Dalian, China.

　　Master program in Applied Chemistry with Profs. Daqing WANG and Tongxin LI with specialization in Fine Organic Synthesis & Heterogeneous Catalysis and the thesis entitled: “Study on the Synthesis of N-Methyltetrafluorophthalimide as a Quinolone Antibacterial Intermediate”.

　　完成的重要项目及得到资助 (1996-现在):

　　1. L-(+)-Ergothioneine (ERT)的制备(2007.4-2007.11, CSE070319, Daicel Chemical Industries, Ltd. (合作项目), 200万日元, 第一完成人）。

　　2. BR-358的研发 (2007.4-2007.11, CSE070314,大日本住友制药有限公司(合作项目), 200万日元, 第一完成人）。

　　3. 由吡咯、咪唑组成的寡肽聚酰胺基因治疗药物的研发 (2006.6-2007.3, CSE060106，日本产、学、研科学技术振兴机构资助（京都大学合作)，700万日元, 第二完成人）。

　　4. 集成电路(Large Scale Integrated (LSI))用的化学材料单聚体ArF的研发(CSE060669, 2006.6-2007.3， 由北海道经济产业局资助, 6000万日元，第二完成人)。

　　5. 由吡咯和咪唑组成的聚酰胺特异性识别DNA序列的分子设计及生物学应用(B14-13218046，2003.12-2006.12, 日本文部科学省21世纪COE项目资助,800万日元; 2006.01-2006.03, 由日本科学技术振兴机构（Japan Science and Technology Agency,JST) 资助, 200万日元，第二完成人)。

　　6. 苯并[k,l]噻吨-3,4-二酰亚胺衍生物作为DNA探针的研究(No. P 01281，2001.11-2003.11, 日本文部科学省学术振兴会（Japan Society for the Promotion of Science, JSPS),1300万日元, 第一完成人）。

　　7. 2,3,4,5-四氟苯甲酸作为喹诺酮抗菌药中间体的合成(1996.3-1998.8，大庆石油管理局，27万，第一完成人)。

　　8. Molecular design, synthesis and application of novel fluorophores for DNA sequencing by hybridization in solution (2000.1-2001.12，由中国大庆市科委资助，6万元，第一完成人)。

　　9. 小分子化合物与G-DNA或B-DNA作用调控癌细胞中关键原癌基因c-myc的表达(上海市化学生物学（芳香杂环）重点实验室的开放基金, SKLCB-2008-04, 2009.01.01-2010.12.31，第一完成人)。

　　10.具有手性修饰的寡肽聚酰胺（polyamide）对DNA碱基的识别和PD-1基因的调控作用(教育部归国留学人员启动基金, 2009.03.01-2011.02.30，第一完成人)。

　　11.聚酰胺（polyamide）对B-DNA碱基识别的研究 (浙江工业大学高层次人才引进科研启动金，2008.03-2012，第一完成人)。

　　12.HIV感染的T细胞高表达PD-1基因的调控诱导免疫功能恢复的研究（卫生部科研基金，合作项目，WKJ2009-2-019，2009.07-2012.06，第二完成人）。

　　13.手性修饰的聚酰胺对介导肿瘤/癌的关键原癌基因c-Kit的调控（浙江省自然科学基金，合作项目，Y2090851，2010.01-2011.12，第二完成人）。

　　14.HIV、HBV、HCV高表达PD-1基因调控鼠模型的建立及应用研究（浙江省公益技术应用研究项目，合作项目，2011C33G1360040，2010.01-2012.12，第二完成人）。

　　目前正在进行的重要项目及得到资助 (2008-现在):

　　1. 手性非alpha-氨基酸衍生物及基因靶标调控物质聚酰胺的研发(浙江省科技厅钱江人才项目, 2008R10G2020015, 2009.01.01-2010.12.31)。

　　2. 重大传染性疾病分子靶向诊疗化合物手性聚酰胺的研发（浙江省“公共卫生监测与突发事件处置关键技术研究科技创新团队”项目，2011R50021，2012.06-2014.05）。

　　3. 结构新颖的手性环型聚酰胺的构建及其对PD-1基因启动子碱基序列的作用（浙江省自然科学基金，LY12B02019，2012.01-2013.12）。

　　4. 浙江省‘十二五’高校“化学生物学”重点学科建设项目（2011.01-2015.12）

　　主要研究方向:

　　1．分子与核酸作用结构生物学解析（Polyamide，Aminoglycoside etc. B-DNA,G-DNA,RNA）

　　2．分子基因调控与靶向药物设计

　　3. 生物活性分子设计及合成

　　4．DNA纳米结构的研究

　　主要教学工作：

　　1. 药物化学（本科）

　　2. 生物化学（本科）

　　3. 化学生物学（研究生）

　　4. 生物制药技术（研究生）

　　目前，“化学生物学与分子药物设计”研究室成员包括：研三（M3）4人，研二（M2）4人，研一（M1）2人，大四本科生（B4）4人。

　　欢迎具有化学和生物基础的本科生、硕士生加入我们的研究团队！研究室将为你提供良好、宽松的学习和研究环境，同时这里也是你迈向科学殿堂的阶梯。

　　Main Original Papers:

　　19.Su-Lin Zhang, Wen Zhang*, G-quadruplex structures and interaction diversity with ligands. Submitted,2013.

　　18. Yan-Ling Wu*, Yan-Ping Ding, Yoshimasa Tanaka and Wen Zhang*. Risk factors and primary prevention trials for type 1 diabetes.International Journal of Biological Sciences,2013，9(6): 666-678.(IF3.168).

　　17.Yan-Ling Wu*, Yan-Ping Ding, Yoshimasa Tanaka, Li-Wen Shen, Nagahiro Minato and Wen Zhang*.γδ T cells and their potential for immunotherapy.Submitted, 2013.

　　16. Yan-Ling Wu*, Ganesh N. Pandian, Yan-Ping Ding, Wen Zhang, Yoshimasa Tanaka and

　　Hiroshi Sugiyama*.Sources of iPS cells and ways to move them from the laboratory to the clinic: Speeding up the journey with small molecules.CHEM BIOL (IF 6.157)，accepted，2013.

　　15. Jing Liang, Yan-Ling Wu, Wen Zhang*, Yoshimasa Tanaka, and Hiroshi Sugiyama.The c-Kit receptor-mediated signal transduction and tumor-related diseases. International Journal of Biological Sciences,2013，9(5):435-443.(IF3.168)

　　14. Dongliang Xie,Junyao He*,Yan-Ling Wu*,Wen Zhang,Yoshimasa Tanaka.The establishment and application of three kinds of the SCID mouse-based improved animal models in the research of AIDS, chronic hepatitis B and C. Advanced Materials Research, 2013，749，433-438.

　　13. Yan-Ling Wu, Jing Liang, Wen Zhang*, Yoshimasa Tanaka, and Hiroshi Sugiyama. Immunotherapies: The Blockade of Inhibitory Signals. International Journal of Biological Sciences, 2012,8(10):1420-1430.(IF3.168,2012)

　　12. Hong-Fei Zhang, Yan-Ling Wu*, Shi-Kun Jiang, Pu Wang, Hiroshi Sugiyama, Xing-Lai Chen, Wen Zhang*, Yan-Juan Ji, and Chuan-Xin Guo.Recognition of the Nonaromatic and Stereochemical Subunit-containing Polyamides to the Four Watson-Crick Base Pairs in the DNA Minor Groove. CHEMBIOCHEM,2012,13,1366-1374.(IF3.74,2012)

　　11. Linan Liu, Yanling Wu*, Shikun Jiang, Tao Qiu, Hongfei Zhang, and Wen Zhang*. Targeting human telomeric G-quadruplex with perfluoroalkyl metal phthalocyanine derivatives. Advanced Materials Research, 2012, 365, 382-388.

　　10. Wen Zhang*, Shi-Kun Jiang, Yan-Ling Wu*, Chuan-Xin Guo, Hong-Fei Zhang, Hiroshi Sugiyama, and Xing-Lai Chen. Discrimination between T/A and A/T Base Pairs of Pyrrole-Imidazole Polyamides Substituted with Chiral β-Hydroxy-γ-Aminobutyric Acid/β-Alanine Pairs. CHEMBIOCHEM, 2012, 13,47-50.(IF3.74,2012)

　　9. Shi-Kun Jiang, Pu Wang, Yan-Ling Wu, and Wen Zhang*. The Role of Aliphatic Chains of Pyrrole-imidazole Polyamides and Their Conjugates Binding to DNA. PROGRESS IN CHEMISTRY, 2011,12(11), 2339-2352. (IF0.67, 2012)

　　8. Yanling Wu, Jing Liang, Lei Chen, Hua Gu, Wen Zhang*, Feng Ke, Yiyu Lu, Ni Wang, Xiaohong Pan. The expression and clinical significance of FOXP3 mRNA in peripheral blood monocytes from HIV/AIDS patients. In: 2010 International Conference on Cellular,Molecular Biology, Biophysics and Bioengineering (CMBB2010, Edited by Xueli Zhou), Qiqihar, China, Institute of Electrical and Electronics Engineers, Inc. (Hong Kong), 2010, 417-420.

　　7. Wen Zhang, Masafumi Minoshima and Hiroshi Sugiyama*. Base pair recognition of the steeochemically alpha-substituted gamma-turn of pyrrole/imidazole hairpin. JACS, 2006, 128, 14905-14912.(IF10.677,2012).

　　6. Wen Zhang, Toshikazu Bando and Hiroshi Sugiyama*. Discrimination of hairpin polyamides with an alpha-substituted-gamma-aminobutyric acid as a TG-reader in DNA minor groove. JACS, 2006, 128(27), 8766-8776.(IF10.677,2012).

　　5. Hiroyuki Matsuda, Noboru Fukuda*, Takahiro Ueno, Yoshiko Tahira, Hirohito Ayame, Wen Zhang, Toshikazu Bando, Hiroshi Sugiyama, Satoshi Saito, Koichi Matsumoto, Hideo Mugishima, and Kazuo Serie. Development of gene silencing pyrrole-imidazole polyamide targeting the TGF-beta 1 promoter for treatment of progressive renal diseases. J Am Soc Nephrol. 2006, 17, 422-432.(IF8.987,2012).

　　4. Wen Zhang, Xuhong Qian*, Yanling Wu and Hiroshi Ohrui. A new class of DNA intercalator and photocleaver: bis-naphthalmides with bromo and nitro substituents. Heterocyclic Comm. 2003, 9(3), 229-234.(IF0.522,2012).

　　3. Wen Zhang, Yanli Wang, Yufang Xu and Xuhong Qian*. New fluorescent conjugates of uridine nucleoside and substituted 1,8-naphthalimide: synthesis, weak interactions and solvent effects on spectra. Monatshefte für Chemie, 2003, 134, 393-402.(IF1.629,2012).

　　2. Ivo Grabchev*, Xuhong Qian, Vladimir Bojinov, Yi Xiao, and Wen Zhang. Synthesis and photophysical properties of 1,8-naphthalimide-labelled PAMAM as PET sensor of protons and of transition metalions. Polymer, 2002, 43 (21), 5731-5736.(IF3.379,2012).

　　1. Qian Cheng*, Wen Zhang, Yoshimichi Tagami, Takayuki Oritani. Microwave-induced 1,3-dipolar intramolecular cycloadditions of N-substituted oximes, nitrones, and azomethine ylides for the chiral synthesis of functionalized nitrogen heterocycles. J. Chem. Soc. Perkin Trans. I,2001,4,452-456.

　　Other publications：

　　1. Wen Zhang, Yanling Wu. The preparative method of tetrachlorophthalic anhydride. 1999, China Patent, CN 1222519A.

　　2. Wen Zhang and Hiroshi Sugiyama. Design of pyrrole-imidazole polyamides discriminating T/A. In: The 4th National Conference on Chemical Biology and the International Symposium on the Interdisciplinary Science of Chemistry with Biology and Medicine, Wuhan, China, 2005, p91.

　　3. Wen Zhang. In: the Annual Reasearch Report of the 21th Century COE Program of Kyoto University Alliance for Chemistry in 2004, p62-63.

　　4. Wen Zhang. In: the Annual Reasearch Report of the 21th Century COE Program of Kyoto University Alliance for Chemistry in 2005, p69-70.

　　5. Wen Zhang and Hiroshi Sugiyama. Synthesis of hairpin polyamides and their biological applications. 2006, Japan patent 10611342.

　　6. Wen Zhang*. A method of preparation of amino and hydroxyl co-protective chiral non-alpha amino acid derivatives. 2006, CN100509766C.

　　7. Shikun Jiang, Yanling Wu, and Wen Zhang*. Recognition of Py-Im Polyamides with Chiral Recognition Unit to Methylated Cytosine. In: The 6th National Conference on Chemical Biology and the International Symposium on the Interdisciplinary Science of Chemistry with Biology and Medicine, Xiamen, China, 2009, p269.

　　8. Yan-Ling Wu*, Yan-Ping Ding, Jian Gao, Lu Fang, Feng Ke, and Wen Zhang*. Suppression of overexpression of c-kit gene by chirally-modified polyamides mediating apoptosis of two types of cancer cells.In: The 7th National Conference on Chemical Biology, Nanjing, China, 2011, p412.

　　9. Wen Zhang*, Yan-Ling Wu*, Shi-Kun Jiang, Chuan-Xin Guo, and Hong-Fei Zhang. Design of the nonaromatic and stereochemical subunit-containing polyamides, and their recognition to PD-1 promoter sequence.In: The 7th National Conference on Chemical Biology, Nanjing, China, 2011, p389.

　　10. Wen Zhang*,Yan-Ling Wu,and Shi-Kun Jiang.一种含或不含β-羟基-γ-氨基酸修饰的发夹聚酰胺、制备方法及应用. 2011, CN 201110454689.1.

　　11. Hong-Fei Zhang, Yi-Qian Jiang, Jia-Hua Yu, Yan-Juan Ji, Yan-Ling Wu, Wen Zhang*. Design, Synthesis of Novel oxo-heterocylic naphtalimides and Their Interaction with guanine-rich telomeric DNA Sequence.In: The 28th National Conference on Chemistry, Chengdu, China, 2012.

　　12. Yan-Ling Wu*, Dong-Long Xie, Yan-Ping Ding, Jian Gao, Wen Zhang*. Design, Synthesis of Chiral Subunit-containing Polyamides and Their Regulation to Overexpressional PD-1 on HIV-, HBV- and HCV-infected Cells.In: The 28th National Conference on Chemistry, Chengdu, China, 2012.

　　13. Wen Zhang*,Yan-Ling Wu,Jing Liang and Bing-Jia Chen.手性（S）-β-羟基-γ-氨基酸修饰的发夹聚酰胺及其应用. 2013, CN 201310118833.3.

　　14.Yan-Juan Ji,Yan-Ling Wu, and Wen Zhang*. Design, Synthesis of Benzothioxanthene Derivatives and Their Mediating Apoptosis of PD-1 Over-expressed Tumor Cells.The 9th AFMC International Medicinal Chemistry Symposium (AIMECS13),Taipei,2013.

　　15.Zhang Su-Lin, Ji Yan-Juan, Wu Yan-Ling*, Zhang Wen*.Suppression of PD-1 gene overexpression by thio-heterocyclic naphthalimides mediating apoptosis of cancer cells.The 8th National Conference on Chemical Biology of China,Shanghai,2013, p 384.

　　16.Chen Bing-Jia, Chen Xing-Lai, Wu Yan-Ling, Zhang Wen*.Downregulation of c-myc gene overexpression by chirally-modified polyamides mediating apoptosis of cancer cells.The 8th National Conference on Chemical Biology of China,Shanghai,2013,p385.

　　Awards & Fellowships:

　　1. 1998年大庆科学技术进步一等奖

　　2. JSPS postdoctoral fellow，Japan, Nov. 2001 to Nov. 2003

　　3. COE postdoctoral fellow, Japan, Dec. 2003 to Dec 2005

　　4. JST postdoctoral fellow, Japan, Jan. 2006 to Mar. 2006

　　5. 2013年湖州市“南太湖精英计划”领军人才