冷冻电镜和结构生物学
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张兴：求是****，博士生导师
浙江大学冷冻电镜平台主任
浙江大学医学院生物物理学系副主任
浙江大学医学院附属邵逸夫医院教授
课题组主要利用冷冻电镜技术研究：
（1）线粒体离子通道的结构和功能关系；
（2）光反应中心超大复合体结构；
（3）病原微生物的核糖体结构与抗生素药物设计；
（4）dsRNA病毒的结构及复制的分子机理。
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应用单颗粒冷冻电镜技术研究病毒、膜蛋白等生物大分子复合体的结构和功能，为药物设计、改进提供结构信息和靶点。主要兴趣：（1）乙肝及丙肝病毒的结构功能;（2）病原微生物的核糖体结构与抗生素药物设计；（3）dsRNA病毒的结构及复制的分子机理;（4）线粒体钙离子通道的结构和作用机理关系。
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2018-06-20 16:46:03
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selfintroduction
A.学习进修经历2007.11-2010.9：美国，加州大学洛杉矶分校，加州纳米研究所，博士后(Hong Z. Zhou)
2005.6-2007.11：美国波士顿，布兰戴斯大学，休斯医学研究所(HHMI)，博士后(Nikolas Grigorieff)
1999.9-2005.6，美国印第安纳州，普度(Purdue)大学，结构生物学专业，博士（Timothy S. Baker）
1993.9-1996.6，中国北京，中科院北京电镜实验室，生物物理专业，硕士（郭可信，徐伟）
1989.9-1993.6，中国成都，四川大学，生物化学专业，本科
B.工作经历
2016.3-至今：中国，浙江大学医学院，冷冻电镜电镜中心主任，教授，博士生导师
2010.10-2016.2：美国，加州大学洛杉矶分校，加州纳米研究所电镜中心，技术主任(Technical Director)
1996.7-1999.7：中国北京，中科院北京电镜实验室，研究助理
C.已取得主要成果：
首次用单颗粒冷冻电镜技术重构出生物大分子复合体的原子结构，确定了单颗粒冷冻电镜作为第三种重构生物大分子原子结构的技术；应用冷冻电镜原位重构策略揭示了双链RNA病毒基因组片段的空间组织方式及其核酸多聚酶调控的分子机理；揭示双链RNA病毒入侵细胞过程中受体结合蛋白和穿膜蛋白协同完成穿膜过程的分子机理。共发表SCI论文22篇，其中10篇为第一作者。总引次数为1685，单篇最高引次数为324。发表的期刊包括Nature、Cell、Nature Structural &Molecular Biology等。其中有四篇第一作者文章被Faculty1000推荐。
D.荣誉
11\/2015 浙江省千人
7\/2014 Selected speaker in Seventh K.H. Kuo Summer School of Electron Microscopy and Crystallography
2\/2013 Invited speaker at the Clinical and Translational Science Lecture, UCLA, USA
8\/2012 Invited speaker in Microscopy and Microanalysis 2012, Phoenix, Arizona, USA
9\/2011 FineGoldaward for postdoctoral research, UCLA, USA
3\/2010 MIMG Departmental nomineefor the chancellor’s award for postdoctoral research, UCLA, USA
6\/2010 Invited speaker of Gordon Research Conference on 3DEM, Italy
8\/2010 Invited speaker of Third K.H. Kuo Summer School of Electron Microscopy and Crystallography, China
E.科研兴趣
应用单颗粒冷冻电镜技术研究病毒、膜蛋白等生物大分子复合体的结构和功能，为药物设计、改进提供结构信息和靶点。主要兴趣：（1）乙肝及丙肝病毒的结构功能;（2）病原微生物的核糖体结构与抗生素药物设计；（3）dsRNA病毒的结构及复制的分子机理;（4）线粒体钙离子通道的结构和作用机理关系。
F.发表文章
1.*Zhang,X., *Lai, M., Chang, W., Yu, I., Ding, K., Mrazek, J., Ng, H.L., Yang, O.O., Maslov, D.A., Zhou,Z.H. (2016). Structures and stabilization of kinetoplastid-specific split rRNAs revealed by comparing leishmanial and human ribosomes. Nature Communications7:13223.(*为共同第一作者)
2.Zhang, X.,Patel, A.; Celma, C.; Yu, X.; Roy, P.; Zhou, H. (2016).Atomic model of a nonenveloped virus reveals pH sensors for a coordinated process of cell entry. Nature Structural & molecular Biology23 (1), 74-80.
3.*Zhang, X., *Ke, D.; *Yu, X.; Chang, W.; Sun, J.; Zhou, H. (2015). In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus. Nature527 (7579), 531–534. (*为共同第一作者)
4.Zhang, X.; Jin, L.; Fang, Q.; Hui, W.; Zhou, Z.H. (2010). 3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry, Cell141(3):472-482. PMCID: PMC**.(featured on cover and PaperFlick video)
5.Zhang, X.Guo, H., Jin L., Czornyj E., Hodes, A., Hui, W.H., Nieh, A.W., Miller, J.F. and Zhou, Z.H. (2013) A new topology of the HK97-like fold revealed in Bordetellabacteriophage by cryoEM at 3.5Å resolution. eLife, 2:e01299.
6.Zhang, X.and Zhou, Z.H. (2011) Limiting factors in atomic resolution cryo electron microscopy – No simple tricks. J. Struct. Biol., 175, 253-263.
7.Zhang, X.; Boyce, M.; Bhattacharya, B.; Zhang, X.; Schein, S.; Roy, P.; Zhou, Z.H. (2010). Bluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins, PNAS107(14):6292-6297.
8.Zhang, X., E. Settembre, C. Xu, P. R. Dormitzer, R. Bellamy, S. C. Harrison and N. Grigorieff (2008). "Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction." PNAS105(6): 1867-1872.
9.Zhang, X.; Ji, Y.; Zhang, L.; Harrison, S. C.; Marinescu, D. C.; Nibert, M. L.; Baker, T. S. (2005). Features of Reovirus Outer Capsid Protein m1 Revealed by Electron Cryomicroscopy and Image Reconstruction of the virion at 7.0-Å Resolution, Structure13(10): 1545-1557.
10.Zhang, X.;Tang, J.; Walker, S. B.;O'Hara, D.; Nibert, M. L.; Duncan, R. and Baker, T. S. (2005). Structure of Avian Orthoreovirus Virion by Electron Cryomicroscopy and Image Reconstruction, Virology343:25-35.
11.Zhang, X.; Walker, S. B.; Chipman, P. R., Nibert, M. L., Baker, T. S. (2003). Reovirus polymerase lambda 3 localized by cryo-electron microscopy of virions at a resolution of 7.6 Å, Nature Structural Biology10(12): 1011-8.
12.Zhang, XK., Zhang, X., Zhou, Z.H. (2010) Low cost, high Performance hybrid GPU-CPU solution for atomic resolution CryoEM single-particle reconstruction. J.Struct. Biol., 172, 400-406.
13.Kim, J.; Zhang, X.; Centonze, V. E., Bowman, V. D., Noble, S., Baker, T. S., Nibert, M. L. (2002). The hydrophilic amino-terminal arm of reovirus core shell protein lambda1 is dispensable for particle assembly, J. Virol.76(23): 12211-22.
14.Luongo, C. L., Zhang, X., Walker, S. B., Chen, Y., Broering, T. J., Farsetta, D. L., Bowman, V. D., Baker, T. S., Nibert, M. L. (2002). Loss of activities for mRNA synthesis accompanies loss of lambda2 spikes from reovirus cores: an effect of lambda2 on lambda1 shell structure, Virology296(1): 24-38.
15.Kong, Y., Zhang, X., Baker, T.S. & Ma, J. A Structural-informatics Approach for Tracing β-Sheets: Building Pseudo-Cα Traces for β-Strands in Intermediate-resolution Density Maps. Journal of molecular biology339, 117-130 (2004).
16.Chandran, K., Zhang X., N. H. Olson, S. B. Walker, J. D. Chappell, T. S. Dermody, T. S. Baker and M. L. Nibert (2001). "Complete in vitro assembly of the reovirus outer capsid produces highly infectious particles suitable for genetic studies of the receptor-binding protein." Journal of virology75(11): 5335-5342.
17.Chen, J; Settembre, E; Aoki, S; Zhang, X; Bellamy, A; Dormitzer; P; Harrison, S; Grigorieff, N. (2009).Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM, PNAS106(26): 10644-10648.
18.Odegard A.; Chandran K.; Zhang X.; Parker J. S.; Baker T. S.; Nibert M. L. (2004). Putative autocleavage of outer capsid protein m1, allowing release of myristoylated peptide m1N during particle uncoating, is critical for cell entry by reovirus, J. Virology.78(16):8732-45. PMCID: PMC479062
19.Yan, X., G. Cardone, Zhang X., Z. H. Zhou and T. S. Baker (2014). "Single particle analysis integrated with microscopy: A high-throughput approach for reconstructing icosahedral particles." Journal of structural biology186(1): 8-18.
20.Mrazek, J., D. Toso, S. Ryazantsev, Zhang, X., Z. H. Zhou, B. C. Fernandez, V. A. Kickhoefer and L. H. Rome (2014). "Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles." ACS nano8(11):11552-9
21.Ji, Y., D. C. Marinescu, W. Zhang, Zhang X., X. Yan and T. S. Baker (2006). "A model-based parallel origin and orientation refinement algorithm for cryoTEM and its application to the study of virus structures." Journal of structural biology154(1): 1-19.
22.Estrozi LF, Settembre EC, Goret G, McClain B, Zhang X, Chen JZ, Grigorieff N, Harrison SC. (2013) Location of the dsRNA-dependent polymerase, VP1, in rotavirus particles. J Mol Biol.425:124-132.
23.Bowman, V. D., Chase, E. S., Franz, A. W., Chipman, P. R., Zhang, X.,Perry, K. L., Baker, T. S., Smith, T. J. (2002). An antibody to the putative aphid recognition site on cucumber mosaic virus recognizes pentons but not hexons, J. Virol.76(23): 12250-8.
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xzhang1999(at)zju.edu.cn
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医学院科研楼A111
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张兴：求是****，博士生导师
浙江大学冷冻电镜平台主任
浙江大学医学院生物物理学系副主任
浙江大学医学院附属邵逸夫医院教授
课题组主要利用冷冻电镜技术研究：
（1）线粒体离子通道的结构和功能关系；
（2）光反应中心超大复合体结构；
（3）病原微生物的核糖体结构与抗生素药物设计；
（4）dsRNA病毒的结构及复制的分子机理。
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