凌 剑（博士，教授，硕士生导师）
专业：分析化学
主讲课程：分析化学、仪器分析、分析化学实验、仪器分析实验等
团队：生命分析化学团队
电话：
电子信箱：lingjian@ynu.edu.cn
受教育经历及工作经历：
2009年8月至今，云南大学化学科学与工程学院 讲师（2009）、副教授（2013）、教授（2019）
2016年8月至2017年8月，华盛顿大学（University of Washington）化学系 访问****
2009年7月，获西南大学 分析化学 博士学位
2004年7月，获西南师范大学 化学 学士学位
教学工作：
主讲《分析化学》、《仪器分析》、《分析化学实验》、《仪器分析实验》等课程
科研领域和兴趣：
研究领域：光学传感新原理、新方法
主要兴趣方向：（1）金属纳米粒子及其组装体的局域表面等离子体共振光谱研究；（2）基于金属纳米簇的荧光、吸收、散射光谱的生物传感研究；（3）钙钛矿发光材料在环境分析中的应用研究；（4）荧光共价/金属有机框架材料的合成及应用研究。
论文及专著：
35. Wang, J.; Hu, Y.-L.; Zhao, R.-X.; Wen, Q.-L.; Wang, J.; Yang, N.; Ling, J.*; Huang, C. Z.; Cao, Q.* Liquid-liquid extraction and visual detection of Hg²⁺ in aqueous solution by luminescent CsPbBr₃ perovskite nanocrystals. Microchemical Journal 2021, 170, 106769.
34. Pu, Z.-F.; Wu, B.-C.; Tan, Y.-H.; Wen, Q.-L.; Ling, J.*; Cao, Q.-E., Selective Aggregation of Silver Nanoprisms Induced by Monohydrogen Phosphate and its Application for Colorimetric Detection of Chromium (III) Ions. Journal of Analysis and Testing 2021, 5 (3), 225-234.
33. Pu, Z.-F.; Peng, J.; Wen, Q.-L.; Li, Y.; Ling, J.*.; Liu, P.; Cao, Q.-E*, Photocatalytic synthesis of BSA-Au nanoclusters with tunable fluorescence for highly selective detection of silver ion. Dyes and Pigments 2021, 193, 109533.
32. Wang, J.; Liu, A.-Y.; Wu, B.-C.; Wen, Q. L.; Pu, Z.-F.; Zhao, R.-X.; Ling, J.*; Cao, Q.*, Highly selective and rapid detection of silver ion by “turn on” a non-fluorescent cysteine stabilized gold nanoclusters probe. Analytical Methods 2021, 13, 2099.
31. Hu, Y.-L.; Liu, A.-Y.; Wu, B.-C.; Zhao, R.-X.; Pu, Z.-F.; Ling, J.*; Cao, Q.* Modulating fluorescence emission of L-methionine-stabilized Au nanoclusters from green to red and its application for visual detection of silver ion. Microchemical Journal. 2021, 166, 106198.
30. Zhao, R.-X.; Liu, A.-Y.; Wen, Q.-L.; Wu, B.-C.; Wang, J.; Hu, Y.-L.; Pu, Z.-F.; Ling, J.*; Cao, Q. Glutathione stabilized green-emission gold nanoclusters for selective detection of cobalt ion. Spectrochimica Acta Part A 2021, 254, 119628
29. Hu, Y.-L.; Wen, Q.-L.; Pu, Z.-F.; Liu, A.-Y.; Wang, J.; Ling, J.*; Xie, X.-G.; Cao, Q.-E.* Rapid synthesis of cesium lead halide perovskite nanocrystals by l-lysine assisted solid-phase reaction at room temperature. RSC Advances 2020, 10 (56), 34215-34224.
28. Wen, Q.-L.; Pu, Z.-F.; Yang, Y.-J.; Wang, J.; Wu, B.-C.; Hu, Y.-L.; Liu, P.; Ling, J.*; Cao, Q*. Hyaluronic acid as a material for the synthesis of fluorescent carbon dots and its application for selective detection of Fe³⁺ ion and folic acid. Microchemical Journal. 2020, 159, 105364.
27. Wen, Q.-L.; Peng, J.; Liu, A.-Y.; Hu, Y.-L.; Wang, J.; Ling, J.*; Cao, Q.-E.*, Fluorescent silver nanoclusters stabilized in guanine-enhanced DNA hybridization for recognizing different small biological molecules. Journal of Luminescence 2020, 221, 117038.
26. Li, Y.; Wen, Q.-L.; Liu, A.-Y.; Long, Y.; Liu, P.; Ling, J.*; Ding, Z.-T.; Cao, Q.-E.*, One-pot synthesis of green-emitting gold nanoclusters as a fluorescent probe for determination of 4-nitrophenol. Microchimica Acta 2020, 187 (2), 106.
25. Wen, Q.-L.; Peng, J.; Liu, A.-Y.; Wang, J.; Hu, Y.-L.; Ling, J.*; Cao, Q.-E., DNA bioassays based on the fluorescence ‘turn off’ of silver nanocluster beacon. Luminescence 2020, 35, 702-708.
24. Pu, Z.-F.; Wen, Q.-L.; Yang, Y.-J.; Cui, X.-M.; Ling, J.*; Liu, P.; Cao, Q.-E., Fluorescent carbon quantum dots synthesized using phenylalanine and citric acid for selective detection of Fe³⁺ ions. Spectrochimica Acta Part A 2020, 229, 117944.
23. Li, X.-J.; Li, Y.; Liu, A.-Y.; Tan, Y.-H.; Ling, J.*; Ding, Z.-T.; Cao, Q.-E.*, Highly selective visual sensing of copper based on fluorescence enhanced glutathione-Au nanoclusters. Spectrochimica Acta Part A 2020, 224, 117472.
22. Peng, J.; Ling, J.*; Wen, Q.-L.; Li, Y.; Cao, Q.-E.*; Huang, Z.-J.; Ding, Z.-T., The presence of a single-nucleotide mismatch in linker increases the fluorescence of guanine-enhanced DNA-templated Ag nanoclusters and their application for highly sensitive detection of cyanide. RSC Advances 2018, 8 (72), 41464-41471.
21. Zhang, X.-Q.; Ling, J.*; Liu, C.-J.; Tan, Y.-H.; Chen, L.-Q.; Cao, Q.-E.*, An irreversible temperature indicator fabricated by citrate induced face-to-face assembly of silver triangular nanoplates. Materials Science and Engineering: C 2018, 92, 657-662.
20. Huang, C. Z.; Ling, J.; Wang, J., Elastic Light Scattering Spectrometry. De Gruyter: Berlin, 2018.
19. Peng, J.; Han, C.-L.; Ling, J.*; Liu, C.-J.; Ding, Z.-T.; Cao, Q.-E.*, Selective fluorescence quenching of papain–Au nanoclusters by self-polymerization of dopamine. Luminescence 2018, 33 (1), 168-173.
18. Peng, J.; Ling, J.*; Tan, Y.-Y.; Jing, C.-J.; Li, X.-J.; Chen, L. Q.; Cao, Q.-E.*, Poly (Thymine)-Templated Copper Nanoparticles as a Fluorescence Probe for Highly Selective and Rapid Detection of Cysteine. Spectroscopy Letters 2017, 50 (3), 137-142.
17. 黄承志，凌剑，王建，弹性光散射光谱分析，科学出版社，北京，2017.
16. Li, X.-J.; Ling, J.*; Han, C.-L.; Chen, L.-Q.; Cao, Q.-E.*; Ding, Z.-T., Chicken Egg White-stabilized Au Nanoclusters for Selective and Sensitive Detection of Hg (II). Analytical Sciences. 2017, 33 (6), 671-675.
15. Chen, B. B.; Liu, H.; Huang, C. Z.; Ling, J.*; Wang, J.*, Rapid and convenient synthesis of stable silver nanoparticles with kiwi juice and its novel application for detecting protease K. New Journal of Chemistry 2015, 39, 1295-1300.
14. Peng, J.; Ling, J.*; Zhang, X.-Q.; Zhang, L.-Y.; Cao, Q.-E.*; Ding, Z.-T., A rapid, sensitive and selective colorimetric method for detection of ascorbic acid. Sensors and Actuators B-Chemical 2015, 221, 708-716.
13. Peng, J.; Ling, J.*; Zhang, X.-Q.; Bai, H.-P.; Zheng, L.; Cao, Q.-E.*; Ding, Z.-T., Sensitive detection of mercury and copper ions by fluorescent DNA/Ag nanoclusters in guanine-rich DNA hybridization. Spectrochim. Acta A 2015, 137, 1250-1257.
12. Zhang, X.-Q.; Peng, J.; Ling, J.*; Liu, C.-J.; Cao, Q.-E.; Ding, Z.-T., Fluorescence resonance energy transfer detection of cobalt ions by silver triangular nanoplates and Rhodamine 6G. Spectroscopy and Spectral Analysis 2015, 35 (4), 951-955.
11. Peng, J.; Yang, X.-H.; Ling, J.*; Liu, C.-J.; Zhang, X.-Q.; Cao, Q.-E.*; Ding, Z.-T., Selective Detection of Mercury (II) by Etching the Corners of Silver Triangular Nanoplates. Spectroscopy Letters 2014, 47 (7), 549-553.
10.Yang, X.-H.; Ling, J.*; Peng, J.; Cao, Q.-E.*; Wang, L.; Ding, Z.-T.; Xiong, J., Catalytic formation of silver nanoparticles by bovine serum albumin protected-silver nanoclusters and its application for colorimetric detection of ascorbic acid. Spectrochimica Acta Part A 2013, 106, 224-230.
9. Yang, X.-H.; Ling, J.*; Peng, J.; Cao, Q.-E.*; Ding, Z.-T.; Bian, L.-C., A colorimetric method for highly sensitive and accurate detection of iodide by finding the critical color in a color change process using silver triangular nanoplates. Analytica Chimica Acta 2013, 798, 74-81.
8. Liu, C.-J.; Ling, J*.; Zhang, X.-Q.; Peng, J.; Cao, Q.-E.*; Ding, Z.-T., Synergistic aggregating of Au(I)-glutathione complex for fluorescence "Turn-on" detection of Pb (II). Analytical Methods 2013, 5, 5584-5588.
7. Ling, J.; Huang, C. Z.* Energy transfer with gold nanoparticles for analytical applications in the fields of biochemical and pharmaceutical sciences. Analytical Methods 2010, 2 (10), 1439-1447.
6. Ling, J.; Li, Y. F.; Huang, C. Z.*, Visual Sandwich Immunoassay System on the Basis of Plasmon Resonance Scattering Signals of Silver Nanoparticles. Analytical Chemistry 2009, 81 (4), 1707-1714.
5. Ling, J.; Huang, C. Z.*; Li, Y. F.; Zhang, L.; Chen, L. Q.; Zhen, S. J., Light-scattering signals from nanoparticles in biochemical assay, pharmaceutical analysis and biological imaging. Trac-Trends in Analytical Chemistry 2009, 28 (4), 447-453.
4. Ling, J.; Sang, Y.; Huang, C. Z.*, Visual colorimetric detection of berberine hydrochloride with silver nanoparticles. Journal of Pharmaceutical and Biomedical Analysis 2008, 47 (4-5), 860-864.
3. Ling, J.; Li, Y. F.; Huang, C. Z.*, A label-free visual immunoassay on solid-support with silver nanoparticles as plasmon resonance scattering indicator. Analytical Biochemistry 2008, 383 (2), 168-173.
2. Ling, J.; Huang, C. Z.*; Li, Y. F.; Long, Y. F.; Liao, Q. G., Recent Developments of the Resonance Light Scattering Technique: Technical Evolution, New Probes and Applications. Applied Spectroscopy Reviews 2007, 42 (2), 177- 201.
1. Ling, J.; Huang, C. Z.*; Li, Y. F., Directly light scattering imaging of the aggregations of biopolymer bound chromium (III) hydrolytic oligomers in aqueous phase and liquid/liquid interface. Analytica Chimica Acta 2006, 567, 143-151.
授权专利：
凌剑、李钰、文秋林. 一种合成巯基b环糊精稳定的绿色荧光金纳米簇的方法及其应用, ZL6.2, 2021.08.13
凌剑、王俊、文秋林、刘安勇、胡怡琳、吴必超. 一种基于荧光钙钛矿液液萃取检测汞离子的方法, ZL3.5, 2021.06.11
凌剑、王俊、胡怡琳、刘安勇、文秋林、吴必超. 一种基于荧光钙钛矿纳米晶体检测重金属离子的方法, ZL2.9, 2020.12.22
凌剑，张秀清，谭远航. 一种快速、简单分离金纳米十面体和金纳米三角片的方法, ZL8.1, 2019.12.17.
主持教学及科研项目：
1. 生命化学大类培养模式下的化学基础实验课程改，2019.01-2020.12，云南省教改项目
2. 光诱导合成荧光可调控的贵金属纳米簇及其分析应用研究，2016.01-2019.12，国家自然科学基金地区项目
3. 银纳米三棱粒子各项异性组装体的等离子体共振耦合光谱研究，2012.01-2014.12，国家自然科学基金青年项目
4. 金属荧光纳米簇在生化分析中的应用研究2011.10-2014.10，云南省应用基础研究项目
5. 基于金纳米粒子的荧光共振能量转移在药物分析中的应用研究，2010.09-2012.12，云南省教育厅科学基金项目
奖励、荣誉和学术兼职：
2020年，Journal of Analysis and Testing 青年编委
2019年，云南省自然科学二等奖（排名第二）
2019年，云南省“****青年拔尖人才计划”
2019年，云南大学“青年英才培育计划”
2017年，云南大学“红云园丁奖”优秀教师奖
2014年，云南大学“中青年骨干教师培育计划”