zww@tju.edu.cn
北洋****-青年骨干教师
副教授
枯草芽孢杆菌合成核黄素的基因组工程研究

---

秸秆水解液生物发酵法制备丁二酸项目

---

用于功能性饲料生产的酿酒酵母菌株的开发

---

大肠杆菌基因组尺度调控因子工程与适应性研究

---

谷氨酸棒杆菌高效共利用葡萄糖和木糖的研究

---

基于高产核黄素枯草芽孢杆菌全基因组突变分析的逆向代谢工程研究

---

生化网络重构与维生素生产新菌种

---

新一代代谢网络模型构建与产品合成最优途径设计

---

高产核黄素枯草芽孢杆菌的逆向代谢工程研究

---

基于RNA-Seq的核黄素高产菌株突变分析与调控机理研究
枯草芽胞杆菌基因组无痕修饰方法

---

枯草芽孢杆菌腺苷琥珀酸合成酶突变基因purA的序列及应用

---

枯草芽孢杆菌双组分调控系统突变基因yvrH及应用

---

枯草芽孢杆菌编码PRPP转酰胺酶突变基因purF及应用
本发明公开了枯草芽孢杆菌基因组无痕修饰方法。本发明的方法利用upp正负筛选系统，对枯草芽孢杆菌基因组进行无痕修饰。本发明的方法可以迭代修饰枯草芽孢杆菌基因组多个目的核酸序列，包括引入基因突变至基因组，从基因组中敲除目的基因序列以及大片段基因组序列删除等。

---

本发明包括枯草芽孢杆菌腺苷琥珀酸合成酶突变基因purA（C725T）编码序列及应用。腺苷琥珀酸合成酶突变基因purA（C725T）编码碱基序列如SEQ ID No.1所示。本发明所构建包含有腺苷琥珀酸合成酶突变基因purA（C725T）的枯草芽孢杆菌工程菌生物安全，遗传背景清晰、包含的腺苷琥珀酸合成酶突变基因purA（C725T）可以大幅提高枯草芽孢杆菌合成核黄素的能力。在摇瓶发酵条件下，可提高核黄素积累水平40%以上。

---

本发明公开了一种枯草芽孢杆菌双组分调控系统突变基因yvrH（G665A）及应用，枯草芽孢杆菌双组分调控系统突变基因yvrH（G665A），是SEQ ID No.1所示。本发明所构建的包含有枯草芽孢杆菌双组分调控系统突变基因yvrH（G665A）的工程菌生物安全，遗传背景清晰、包含的突变基因yvrH（G665A）可以大幅提高枯草芽孢杆菌合成核黄素的能力，可在摇瓶发酵条件下，提高核黄素积累水平15%以上。

---

本发明公开了枯草芽孢杆菌编码PRPP转酰胺酶突变基因purF及应用，枯草芽孢杆菌编码PRPP转酰胺酶突变基因purF序列用SEQ ID No.1所示。本发明所构建的包含有枯草芽孢杆菌编码PRPP转酰胺酶突变基因purF的工程菌生物安全，遗传背景清晰，可以大幅提高枯草芽孢杆菌合成核黄素的能力，提高核黄素积累水平20%以上。
Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene f

---

Construction, model-based analysis, and characterization of a promoter library for fine-tuned gene expression in Bacillus sub

---

Enhancement of 5-aminolevulinic acid production by metabolic engineering of the glycine biosynthesis pathway in Corynebacteri

---

Pathway-consensus approach to metabolic network reconstruction for Pseudomonas putida KT2440 by systematic comparison of publ

---

Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis.

---

Integrated whole-genome and transcriptome sequence analysis reveals the genetic characteristics of a riboflavin-overproducing

---

Concomitant cell-free biosynthesis of optically pure D-(-)-acetoin and xylitol via a novel NAD+ regeneration in two-enzyme ca

---

Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

---

Model-based reconstruction of synthetic promoter library in Corynebacterium glutamicum

---

Systematic metabolic engineering of Corynebacterium glutamicum for industrial-level production of optically pure D-(-)-Acetoi

---

Genome-scale metabolic model analysis indicates low energy production efficiency in marine ammonia-oxidizing archaea

---

Deregulation of purine pathway in Bacillus subtilis and its use in riboflavin biosynthesis

---

Metabolic engineering of Escherichia coli for the production of riboflavin

---

Establishment of a markerless mutation delivery system in Bacillus subtilis stimulated by a double-strand break in the chromo

---

NADH plays the vital role for chiral pure D-(-)-2,3-Butanediol production in Bacillus subtilis under limited oxygen condition

---

Engineering microorganisms based on molecular evolutionary analysis: a succinate production case study

---

Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis,

---

Development of a markerless gene replacement system in Corynebacterium glutamicum using upp as a counter-selection marker

---

Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate productivity u

---

大肠杆菌氧化还原辅因子代谢工程

---

微生物代谢组学的研究方法与进展

---

Production of Acetoin through Simultaneous Utilization of Glucose, Xylose, and Arabinose by Engineered Bacillus subtilis

---

High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric max

---

Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production

---

Directed evolution of adenylosuccinate synthetase from Bacillus subtilis and its application in metabolic engineering

---

Metabolic engineering of Corynebacterium glutamicum for efficient production of 5-aminolevulinic acid

---

Selective pressure on synonymous codon usage in mammalian protein-encoding genes

---

The research progress of protein directed evolution

---

多元模块工程在代谢工程中的应用与研究进展

---

人工合成启动子文库研究进展

---

嘌呤核苷及其衍生物的代谢工程

---

逆向代谢工程的最新研究进展