gywang@tju.edu.cn
未公开
教授
深海真核微生物新资源的获取与应用潜力评估

---

破囊壶菌DHA产业化中试技术研究

---

我国近海典型水域破囊壶菌的多样性及其在碳、氮生物地球化学循环中的作用

---

深圳邻近海域细菌污染调查及评价

---

深海微生物基因组测序分析

---

陆源病原微生物入海传播扩散机制及其风险评估预测

---

秦皇岛入海排污口及河口生态环境调查

---

破囊壶菌二十二碳六烯酸（DHA）生物合成路径及代谢网络的研究

---

国家海洋公益项目“北戴河邻近海域典型生态灾害与污染监控关键技术集成应用”合同项目

---

近海生态系统结构与碳循环功能

---

入海排污流量监控系统设计研发项目

---

多参数浮标式海洋环境实时监测系统

---

入海排污流量监控系统设计研发项目（补充协议）

---

破囊壶菌活性产物关键工程化技术研发与示范应用

---

基于间断化学分析技术的全自动海水营养盐在线分析仪

---

秦皇岛海域病原菌现状和污染物调查

---

北戴河及相邻地区近岸海域综合整治-2012年海洋环境保障工程

---

近海水域破囊壶菌多样性及其生物医学生物技术应用价值研究

---

中国近海海洋环境对气候变化的影响过程与机制研究
一种破囊壶菌的分离培养基及分离纯化方法

---

基于流式细胞检测技术的破囊壶菌快速测定方法

---

一种近岸海水中副溶血性弧菌的检测方法

---

一种近岸海水中大肠杆菌的检测方法

---

一种高产胞外多糖的海洋破囊壶菌菌株分离筛选方法

---

一种隐球酵母的分离纯化培养及鉴定方法

---

一种高产角鲨烯的破囊壶菌发酵培养基及其培养方法

---

一种高产DHA的破囊壶菌发酵培养基及其培养方法

---

一种破囊壶菌高产脂肪酸的发酵培养基及其应用

---

一种耐盐弗氏柠檬酸杆菌的筛选培养基及培养方法
本发明公开了一种破囊壶菌的分离培养基及分离纯化方法，其分离培养基是用下述方法制成：称取葡糖糖、酵母提取物、蛋白胨、玉米浆、谷氨酸钠、琼脂、人工海盐，加入超纯水并搅拌均匀，灭菌，无菌条件下，将温度降至50+5℃，加入现配制的经0.22um滤膜过滤的抗生素混合液，摇匀，倒平板，即得破囊壶菌的分离培养基；所述抗生素混合液是用下述方法制成：称取氨苄西林0.5-1g，链霉素0.5-1.5g，制酶菌素5-20mg，加超纯水至5ml，溶解并摇匀至溶液澄清，得抗生素混合液。

---

本发明公开了一种基于流式细胞检测技术的破囊壶菌快速测定方法，包括如下步骤：（1）取样和样品前处理：将样品用除菌液稀释或固定，待测样品用吖啶黄溶液避光染色，同时，配制荧光微球悬液作为内参；（2）流式细胞检测：使用配备488nm激光器的流式细胞仪测试样品；（3）内参荧光微球计数；用纯水稀释荧光微球悬液，涡旋振荡混匀后黑色聚碳酸脂膜上，制作玻片，用荧光显微镜对内参荧光微球进行绝对计数；（4）破囊壶菌丰度计数：使用Flowjo或cellQuest软件分析流式数据，主要包括破囊壶菌的圈门和计数。

---

本发明公开了一种近岸海水中副溶血性弧菌的检测方法，包括环境样品DNA提取、扩增目的片段、构建标准质粒模板，建立Q-PCR定量标准曲线、环境样品副溶血性弧菌数测定五个步骤。与当下基于选择性培养法检测相比，该方法有以下优点：首先，缩短了检测时间，由原方法的10-12天，缩短为1天，在完成标准曲线的情况下仅需3-4个小时。其次，克服了原方法无法测定不可培养副溶血性壶菌的问题，提高了鉴别的准确度。

---

本发明公开了一种近岸海水中大肠杆菌的检测方法，包括大肠杆菌DNA提取、扩增目的片段、构建标准质粒模板、建立Q-PCR定量标准曲线、环境样品大肠杆菌数测定五个步骤，与当下基于选择性培养法检测相比，本发明方法的10-12天，缩短为1天，在完成标准曲线的情况下仅需3-4个小时。其次，克服了原方法无法测定不可培养大肠杆菌的问题，提高了鉴别的准确度。

---

本发明公开一种高产胞外多糖（EPS）海洋破囊壶菌菌株分离筛选方法，主要步骤包括制备分离培养基，分离纯化，制备液体培养基，摇瓶发酵培养初筛菌液，制备初筛胞外液，培养复筛菌液，制备二次筛选的胞外液，以及微量苯酚硫酸法测定EPS含量。本发明为分离红树林潮间带来源破囊壶菌菌株及筛选高产胞外多糖的破囊壶菌菌株提供了技术支持，其次，本发明采用初筛、纯化和复筛结合的方法高效快速的分离高产EPS的菌株，且确定了所筛选菌株EPS产量最高的发酵培养时间。

---

本发明公开一种隐球酵母的分离纯化培养及鉴定方法，主要包括制备分离培养基及液体培养基，分离纯化，DNA提取，扩增目的片段及测序以及对PCR产物进行琼脂糖凝胶电泳检测，并切胶回收目的片段测序结果与GeneBank中隐球酵母Cryptococcus albidus 达到99%相似性。本发明能方便地从潮间带红树林腐败落叶样品中分离出隐球酵母，培养基成本低，分离操作简单。对于目前隐球酵母的分离来源多为陆地土壤、陆地植物及陆生植物果实，鲜件海洋底泥。

---

本发明属于培养基技术领域，尤其涉及一种高产角鲨烯的破囊壶菌发酵培养基及其培养方法，方法步骤包括：发酵培养基制备，种子液制备，摇瓶发酵培养发酵液，测定干重和角鲨烯含量。此培养基与原始培养基相比，培养基成分有所减少，且NaCl浓度减少了76%，进行大规模发酵培养时，可降低对发酵设备的腐蚀，节约成本；破囊壶菌生产角鲨烯产量低限制了其工业化生产，优化其发酵生产培养基是提高产量的重要手段，本发明是提出一种利用破囊壶菌高产角鲨烯的培养基成分及配比，提高角鲨烯的发酵产量，为大规模发酵生产提供依据。

---

本发明属于培养基技术领域，尤其涉及一种高产DHA的破囊壶菌发酵培养基及其培养方法，培养方法步骤主要包括破囊壶菌发酵培养基M4tju制备和通用培养基M4制备，菌种活化，种子液制备，摇瓶发酵培养发酵液，测量生物量干重以及DHA产量。本发明提供发酵培养基及培养条件简单，操作方便，生产成本低，DHA产量高，易于工业化生产，节能环保，经济和社会效益巨大。

---

无

---

无
Mining terpenoid production and biosynthetic pathway in thraustochytrids

---

Flow cytometry for rapid enumeration of thraustochytrid protists in coastal seawaters

---

Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northe

---

Seasonal influence of scallop culture on nutrient flux, bacterial pathogens and bacterioplankton diversity across estuaries o

---

Bio-based squalene production by Aurantiochytrium sp. through optimization of culture conditions, and elucidation of the puta

---

Toward a better guard of coastal water safety—Microbial distribution in coastal water and their facile detection

---

Abundance and Molecular diversity of thraustochytrids in coastal waters of Southern China

---

Distinct seasonal patterns of bacterioplankton abundance and dominance of phyla α-Proteobacteria and Cyanobacteria in Qinhuan

---

Spatiotemporal variations and factors affecting molecular diversity and abundance of planktonic fungi in the coastal waters o

---

Comparative analysis reveals unexpected genome features of newly isolated thraustochytrids strains: on ecological function an

---

Enhanced saturated fatty acids accumulation in cultures of newly-isolated strains of Schizochytrium sp. and Thraustochytriida

---

High phylogenetic diversity and abundance pattern of Labyrinthulomycete protists in the coastal waters of the Bohai Sea

---

Molecular detection and spatiotemporal characterization of Labyrinthulomycete protist diversity in the coastal waters along t

---

A High-Resolution Time Series Reveals Distinct Seasonal Patterns of Planktonic Fungi at a Temperate Coastal Ocean Site (Beauf

---

Gradients of three coastal environments off the South China Sea and their impacts on the dynamics of heterotrophic microbial

---

Storm runoff differentially influences the nutrient concentrations and microbial contamination at two distinct beaches in nor

---

Impact of environmental gradients on the abundance and diversity of planktonic fungi across coastal habitats of contrasting t

---

Generating power from cellulose in an alkaline fuel cell enhanced by methyl viologen as an electron-transfer catalyst

---

Culturable diversity and biochemical features of thraustochytrids from coastal waters of Southern China

---

Ecological dynamics and biotechnological implications of thraustochytrids from marine habitats

---

Spatial diversity of bacterioplankton communities in surface water of Northern South China Sea

---

Distribution and Diversity of Planktonic Fungi in the West Pacific Warm Pool

---

Diversity and biochemical features of culturable fungi from the coastal waters of Southern China

---

Mechanisms of fatty acid synthesis in marine fungus-like protists

---

The POM-DOM piezophilic microorganism continuum (PDPMC)—The role of piezophilic microorganisms in the global ocean carbon cyc

---

Diversity of parasitic fungi associated with phytoplankton in Hawaiian waters

---

Mining terpenoids production and biosynthetic pathway in thraustochytrids

---

Distinct seasonal patterns of bacterioplankton abundance and dominance of phyla α-proteobacteria and cyanobacteria in Qinhuan

---

Abundance and molecular diversity of thraustochytrids in coastal waters of southern China

---

Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp.form coastal water of Norther

---

Toward a better guard of coastal water safety-microbial distribution in coastal water and their facile detection.

---

Seasonal influence of Scallop culture on nutrients flux, bacterial pathogens and plankton diversity across estuaries off Boha

---

Oceaniglobus indicus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from surface seawater

---

Improvement of 1,3-propanediol oxidoreductase (DhaT) stability against 3-hydroxypropionaldehyde by substitution of cysteine r

---

Enhanced Saturated Fatty Acids Accumulation in Cultures of Newly-isolated Strains of Schizochytrium sp. and Thraustochytriida

---

Spatiotemporal variations and factors affecting molecular diversity and abundance of planktonic fungi in the coastal waters

---

Flow cytometry for rapid enumeration of thraustochytrid protists in coastal seawaters

---

秦皇岛新开河河口邻近海域细菌多样性及其影响因子

---

降雨对秦皇岛西浴场细菌总数和可培养菌群组成的影响

---

深圳近海表层浮游细菌分布特征及其环境影响因素

---

单细胞原生生物在海洋碳汇研究中的重要性和展望