姓名 张锴 职称 教授 所在
部门 生物化学与分子生物学系 研究
方向 蛋白质组学和肿瘤生物学
办公室 407 办公
电话 电子
邮箱 kzhang@tmu.edu.cn
教育背景
2000年9月至2003年6月 南开大学化学学院 博士
1997年9月至2000年6月 南开大学化学学院 硕士
1992年9月至1996年6月 南开大学化学学院 本科


工作经历
2014-今 天津医科大学 基础医学院生物化学与分子生物学系 教授
2012-2013 南开大学 药物化学生物学国家重点实验室 PI
2011-2011 美国芝加哥大学 访问****
2008-2013 南开大学 化学学院 副教授
2006-2008 美国德克萨斯大学西南医学中心 博士后
2004-2006 美国华盛顿州立大学 博士后
2003-2004 法国国家科研中心 CNRS-7575（巴黎）博士后

研究成果（本人具有代表性的论著、论文及主持的科研项目）
论著
及编
著 　

论文 　(1) Dong, H.Y., Zhai, G. J., Chen, C., Bai, X., Tian, S. S., Hu, D.Q., Fan, E. G., Zhang, K.* (2019) Protein lysine de-2-hydroxyisobutyrylation by CobB in prokaryote, Science Advances, 5: eaaw6703.
　(2) An, J.Y., Zhai, G.J.*, Guo, Z.C., Bai, X., Chen, P., Dong, H.Y., Tian, S.S., Ai, D., Zhang, Y.K., and Zhang, K.* (2019) Combinatorial Peptide Ligand Library-Based Photoaffinity Probe for the Identification of Phosphotyrosine-Binding Domain Proteins. Analytical Chemistry 91, 3221-3226.
　(3) Zhai, G., Dong, H., Guo, Z., Feng, W., Jin, J., Zhang, T., Chen, C., Chen, P., Tian, S., Bai, X., Shi, L., Fan, E., Zhang, Y., and Zhang, K.* (2018) An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes. Analytical Chemistry 90, 11385-11392
　(4) Bai, X., Bi, W. J., Dong, H. Y., Chen, P., Tian, S. S., Zhai, G. J., and Zhang, K.* (2018) An Integrated Approach Based on a DNA Self-Assembly Technique for Characterization of Crosstalk among Combinatorial Histone Modifications. Analytical Chemistry 90, 3692-3696
　(5) Dong, H. Y., Guo, Z. C., Feng, W., Zhang, T., Zhai, G. J., Palusiak, A., Rozalski, A., Tian, S. S., Bai, X., Shen, L. J., Chen, P., Wang, Q., Fan, E. G., Cheng, Z. Y., and Zhang, K.* (2018) Systematic Identification of Lysine 2-hydroxyisobutyrylated Proteins in Proteus mirabilis. Mol Cell Proteomics 17, 482-494
　(6) Shen, L., Chen, C., Guo, Z., Zhai, G., Dong, H., Li, M., Zhang, K.* (2018) Abnormal expression of proteins in human renal mesangial cells stimulated by high levels of uric acid. Int J Clin Exp Med 9, 9434-9440.
　(7) Su, D. X., Ma, S., Shan, L., Wang, Y., Wang, Y. J., Cao, C., Liu, B. B., Yang, C., Wang, L. Y., Tian, S. S., Ding, X., Liu, X. H., Yu, N., Song, N., Liu, L., Yang, S. D., Zhang, Q., Yang, F. Q., Zhang, K., and Shi, L. (2018) Ubiquitin-specific protease 7 sustains DNA damage response and promotes cervical carcinogenesis. Journal of Clinical Investigation 128, 4280-4296
　(8) He, J. L., Bao, Q. K., Zhang, Y., Liu, M. M., Lv, H. Z., Liu, Y. J., Yao, L., Li, B. C., Zhang, C. H., He, S., Zhai, G. J., Zhu, Y., Liu, X., Zhang, K., Wang, X. J., Zou, M. H., Zhu, Y., and Ai, D. (2018) Yes-Associated Protein Promotes Angiogenesis via Signal Transducer and Activator of Transcription 3 in Endothelial Cells. Circulation Research 122, 591-605
　(9) Bi, C., Liang, Y., Shen, L., Tian, S., Zhang, K., Li, Y., He, X., Chen, L., and Zhang, Y. (2018) Maltose-Functionalized Hydrophilic Magnetic Nanoparticles with Polymer Brushes for Highly Selective Enrichment of N-Linked Glycopeptides. ACS Omega 3, 1572-1580
　(10) Yang, S. D., Liu, L., Cao, C., Song, N., Wang, Y. J., Ma, S., Zhang, Q., Yu, N., Ding, X., Yang, F. Q., Tian, S. S., Zhang, K., Sun, T., Yang, J., Yao, Z., Wu, S. Y., and Shi, L. (2018) USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. Nature Communications 9, 1285
　(11) Fu, X., Zhang, C. Y., Meng, H., Zhang, K., Shi, L., Cao, C., Wang, Y., Su, C., Xin, L. B., Ren, Y. Y., Zhang, W., Sun, X. M., Ge, L., Silvennoinen, O., Yao, Z., Yang, X., and Yang, J. (2018) Oncoprotein Tudor-SN is a key determinant providing survival advantage under DNA damaging stress. Cell Death and Differentiation 25, 1625-1637
　(12) Gao, D., Wang, L., Zhang, H., Yan, X., Yang, J., Zhou, R., Chang, X., Sun, Y., Tian, S., Yao, Z., Zhang, K., Liu, Z., and Ma, Z. (2018) Spleen tyrosine kinase SYK(L) interacts with YY1 and coordinately suppresses SNAI2 transcription in lung cancer cells. FEBS J, doi: 10.1111/febs.14665
　(13) Gao, J., Yang, Y., Qiu, R., Zhang, K., Teng, X., Liu, R., and Wang, Y. (2018) Proteomic analysis of the OGT interactome: novel links to epithelial-mesenchymal transition and metastasis of cervical cancer. Carcinogenesis 39, 1222-1234
　(14) Qiu, R. F., Shi, H., Wang, S., Leng, S., Liu, R. Q., Zheng, Y., Huang, W., Zeng, Y., Gao, J., Zhang, K., Hou, Y. Q., Feng, D. D., and Yang, Y. (2018) BRMS1 coordinates with LSD1 and suppresses breast cancer cell metastasis. Am J Cancer Res 8, 2030-2045
　(15) Tian, S. S., Zheng, S. Z., Han, Y. P., Guo, Z. C., Zhai, G. J., Bai, X., He, X., Fan, E. G., Zhang, Y. K., and Zhang, K.* (2017) Maleic Anhydride Labeling-Based Approach for Quantitative Proteomics and Successive Derivatization of Peptides. Analytical Chemistry 89, 8259-8265
　(16) Bi, W., Bai, X., Gao, F., Lu, C., Wang, Y., Zhai, G., Tian, S., Fan, E., Zhang, Y., and Zhang, K.* (2017) DNA-Templated Aptamer Probe for Identification of Target Proteins. Analytical Chemistry 89, 4071-4076
　(17) Lu, C. C., Tian, S. S., Zhai, G. J., Yuan, Z. F., Li, Y. J., He, X. W., Zhang, Y. K., and Zhang, K.* (2017) Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry. ACS Chemical Biology 12, 57-62
　(18) He, J. Z., Wu, Z. Y., Wang, S. H., Ji, X., Yang, C. X., Xu, X. E., Liao, L. D., Wu, J. Y., Li, E. M., Zhang, K.*, and Xu, L. Y.* (2017) A decision tree based combination of ezrin-interacting proteins to estimate the prognostic risk of patients with esophageal squamous cell carcinoma. Hum Pathol 66, 115-125
　(19) Lin, L. H., Xu, Y. W., Huang, L. S., Hong, C. Q., Zhai, T. T., Liao, L. D., Lin, W. J., Xu, L. Y., Zhang, K., Li, E. M., and Peng, Y. H. (2017) Serum proteomic-based analysis identifying autoantibodies against PRDX2 and PRDX3 as potential diagnostic biomarkers in nasopharyngeal carcinoma. Clinical Proteomics 14, doi: 10.1186/s12014-017-9141-5
　(20) Mu, Y., Yan, X., Li, D., Zhao, D., Wang, L., Wang, X., Gao, D., Yang, J., Zhang, H., Li, Y., Sun, Y., Wei, Y., Zhang, Z., Chang, X., Yao, Z., Tian, S., Zhang, K., Terada, L. S., Ma, Z., and Liu, Z. (2017) NUPR1 maintains autolysosomal efflux by activating SNAP25 transcription in cancer cells. Autophagy, 1-17
　(21) Guo, Y. X., Zhang, Z. S., Wei, H. T., Wang, J. Y., Lv, J. Q., Zhang, K., Keller, E. T., Yao, Z., and Wang, Q. (2017) Cytotoxic necrotizing factor 1 promotes prostate cancer progression through activating the Cdc42-PAK1 axis. Journal of Pathology 243, 208-219
　(22) Bai, X., Lu, C. C., Jin, J., Tian, S. S., Guo, Z. C., Chen, P., Zhai, G. J., Zheng, S. Z., He, X. W., Fan, E. G., Zhang, Y. K., and Zhang, K.* (2016) Development of a DNA-Templated Peptide Probe for Photoaffinity Labeling and Enrichment of the Histone Modification Reader Proteins. Angewandte Chemie-International Edition 55, 7993-7997
　(23) Zheng, S. Z., Bai, X., Tian, S. S., Wang, G. J., Zhai, G. J., Guo, Z. C., Bi, W. J., Shen, L. J., and Zhang, K.* (2016) Identification of hydroxylation at aromatic amino acid residues in yeast kinase using mass spectrometry with affinity enrichment. Rapid Communications in Mass Spectrometry 30, 185-189
　(24) Zheng, S. Z., Sun, M., Zhang, K.*, Gu, J. J., Guo, Z. C., Tian, S. S., Zhai, G. J., He, X. W., Jin, Y.*, and Zhang, Y. K.* (2016) Profiling post-translational modifications of histones in neural differentiation of embryonic stem cells using liquid chromatography-mass spectrometry. J Chromatogr B 1017, 36-44
　(25) Duan, Y., Huo, D. W., Gao, J., Wu, H., Ye, Z., Liu, Z., Zhang, K., Shan, L., Zhou, X., Wang, Y., Su, D. X., Ding, X., Shi, L., Wang, Y., Shang, Y. F., and Xuan, C. H. (2016) Ubiquitin ligase RNF20/40 facilitates spindle assembly and promotes breast carcinogenesis through stabilizing motor protein Eg5. Nature Communications 7, 12648
　(26) Wang, Q., Ma, S., Song, N., Li, X., Liu, L., Yang, S. D., Ding, X., Shan, L., Zhou, X., Su, D. X., Wang, Y., Zhang, Q., Liu, X. H., Yu, N., Zhang, K., Shang, Y. F., Yao, Z., and Shi, L. (2016) Stabilization of histone demethylase PHF8 by USP7 promotes breast carcinogenesis. Journal of Clinical Investigation 126, 2205-2220
　(27) Yang, X., Dong, X. C., Zhang, K., Yang, F. F., and Guo, Z. C. (2016) A molecularly imprinted polymer as an antibody mimic with affinity for lysine acetylated peptides. J Mater Chem B 4, 920-928
　(28) Zheng, S. Z., Zhang, K.*, Tian, S. S., He, X. W., and Zhang, Y. K.* (2015) Identification of Two Novel Modifications at Tryptophan Residues. Journal of the American Society for Mass Spectrometry 26, 1787-1790
　(29) Zhang, K.*, Li, L. Y., Zhu, M. X., Wang, G. J., Xie, J. J., Zhao, Y. L., Fan, E. G., Xu, L. Y.*, and Li, E. M.* (2015) Comparative analysis of histone H3 and H4 post-translational modifications of esophageal squamous cell carcinoma with different invasive capabilities. Journal of Proteomics 112, 180-189
　(30) Wang, Y., Han, Y. P., Fan, E. G.*, and Zhang, K.* (2015) Analytical strategies used to identify the readers of histone modifications: A review. Analytica Chimica Acta 891, 32-42
　(31) Han, Y. P., Lu, C. C., Zhang, K.*, Tian, S. S., Fan, E. G., Chen, L. X., He, X. W., and Zhang, Y. K. (2015) Quantitative characterization of histone post-translational modifications using a stable isotope dimethyl-labeling strategy. Analytical Methods 7, 3779-3785
　(32) Li, L. Y., Zhang, K., Jiang, H., Xie, Y. M., Liao, L. D., Chen, B., Du, Z. P., Zhang, P. X., Chen, H., Huang, W., Jia, W., Cao, H. H., Zheng, W., Li, E. M., and Xu, L. Y. (2015) Quantitative proteomics reveals the downregulation of GRB2 as a prominent node of F806-targeted cell proliferation network. Journal of Proteomics 117, 145-155
　(33) Bi, C. F., Zhao, Y. R., Shen, L. J., Zhang, K., He, X. W., Chen, L. X., and Zhang, Y. K. (2015) Click Synthesis of Hydrophilic Maltose-Functionalized Iron Oxide Magnetic Nanoparticles Based on Dopamine Anchors for Highly Selective Enrichment of Glycopeptides. ACS Applied Materials & Interfaces 7, 24670-24678
　(34) Zhao, Y. L., Fang, X. L., Wang, Y., Zhang, J. M., Jiang, S., Liu, Z., Ma, Z. Y., Xu, L. Y., Li, E. M., and Zhang, K.* (2014) Comprehensive Analysis for Histone Acetylation of Human Colon Cancer Cells Treated with a novel HDAC Inhibitor. Curr Pharm Design 20, 1866-1873
　(35) Lu, C. C., Zhang, K.*, Zhang, Y., Tan, M. J., Li, Y. J., He, X. W., and Zhang, Y. K. (2014) Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins. Journal of Chromatography A 1372, 34-41
　(36) Zhang, H. F., Zhang, K., Liao, L. D., Li, L. Y., Du, Z. P., Wu, B. L., Wu, J. Y., Xu, X. E., Zeng, F. M., Chen, B., Cao, H. H., Zhu, M. X., Dai, L. H., Long, L., Wu, Z. Y., Lai, R., Xu, L. Y., and Li, E. M. (2014) miR-200b suppresses invasiveness and modulates the cytoskeletal and adhesive machinery in esophageal squamous cell carcinoma cells via targeting Kindlin-2. Carcinogenesis 35, 292-301
　(37) Zhang, K.*, Tian, S. S., and Fan, E. G.* (2013) Protein lysine acetylation analysis: current MS-based proteomic technologies. Analyst 138, 1628-1636
　(38) Zhang, K.*, Zheng, S. Z., Yang, J. S., Chen, Y., and Cheng, Z. Y. (2013) Comprehensive Profiling of Protein Lysine Acetylation in Escherichia coli. Journal of proteome research 12, 844-851
（#:第一作者；*:通讯作者）

科研
项目 　国家自然科学基金面上项目，组蛋白翻译后修饰识别蛋白系统鉴定新方法再研究，项目编号：**，2019-2022，主持。
　天津市自然科学基金重点项目，食管癌组蛋白修饰特异性识别蛋白分子网络研究，2019-2022，主持。
　国家自然基金面上项目，组蛋白翻译后修饰及其结合蛋白系统鉴定新方法的研究，项目编号：**，2013-2016，主持。
　国家自然基金面上项目，蛋白激酶中非磷酸化修饰形式鉴定的系统研究，项目编号：**，2010-2012，主持。
　国家自然基金重大研究计划培育项目，系统分析诱导性多能干细胞组蛋白翻译后修饰及与体细胞和胚胎干细胞的比较，项目编号：**，2010-2012，主持。
　天津市自然科学基金，食管癌侵袭相关表观遗传标志物的鉴定和应用，项目编号：14JCYBJC24000，2014-2017，主持。
　国家重点基础研究发展计划（973），赖氨酸翻译后修饰及对蛋白质功能的调控作用，项目编号：2013CB910903，2013-2017，参与。

荣誉奖励


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