一、个人基本信息
周恩民，男，现任西北农林科技大学****，2011年入选陕西省人才项目，农业部兽用药物与兽医生物技术陕西科学观测实验站主任。现担任陕西省动物疫情应急防治队专家组副组长、国务院学位委员会第七届学科评议组（兽医学）成员、第九届农业部科学技术委员会委员、教育部高等学校动物医学专业教学指导委员会副主任委员、第四届国家转基因生物安全委员会委员、农业部基因克隆与转基因技术执行专家组成员、农业部全国动物卫生风险评估专家委员会委员、中国免疫学会兽医免疫分会第五届委员会副主任委员、中国畜牧兽医学会家畜传染病学分会常务理事、中国畜牧兽医学会兽医公共卫生学分会常务理事。
二、教育及研究经历
1993年毕业于加拿大明尼多巴大学免疫学系，获博士学位，1985年至1988年美国德可萨思州圣安东尼奥西南生物医学研究所病毒免疫学系访问****。1993年至2010年曾任加拿大国家食品检验检疫总局国家外来病中心免疫诊断实验室研究员、高级研究员和主任、美国衣阿华州立大学兽医学院兽医诊断和动物生产医学系副教授兽医诊断实验室血清诊断实验室主任、山东省“泰山****海外特聘专家”等职。受聘英国利物浦大学感染生物学系荣誉访问教授，聘期自2019年6月19日至2022年5月31日。
三、研究方向及开设课程
研究方向1：猪繁殖与呼吸综合征。猪繁殖与呼吸综合征病毒生物学、病毒与宿主互作、免疫调控、诊断试剂疫苗及生物制剂研发。
研究方向2：戊型肝炎。禽和猪戊型肝炎病毒生物学、病毒与宿主互作、致病机制、病毒跨种系传播机制、诊断试剂疫苗及生物制剂研发。
开设课程：本科生《兽医免疫学》及全英文《兽医免疫学》；研究生《兽医免疫学研究进展》、《兽医学专题》。
四、代表性学术论著（近5年）
25. Shona Moore, Rebekah Penrice-Randal, Muhannad Alrumaili, Nadine Ranle, Stuart Armstrong, Catherine Hartley, Sam Haldenby, Xiaofeng Dong, Abdulrahman Alrezaihi, Mai Almsaud, Eleanor Bentley, Jordan Clark, Isabel Garcia-Dorival, Paul Gilmore, Ximeng Han, Benjamin Jones, Lisa Luu, Parul Sharma, Ghada Shawli, Yani Sun, Qin Zhao, Stewen Pullan, Daniel Carter, Kevin Bewley, Jake Dunning, En-min Zhou, Tom Solomon, Michael Beadsworth, James Cruise, Derrick Crook, David Matthews, Andrew Dadidson, Zana Mahmood, Waleed Aljabr, Julian Druce, Richard Vipond, Lisa Ng, Laurent Renia, Peter Openshaw, Kenneth Baillie, Miles Carroll, James Stewart, Alistair Darby, Malcolm Semple, Lance Turtle and Julian Hiscox. Ampliccon-based detection and sequencing of SARS-CoV-2 in nasopharyngeal swabs from patients with COVID-19 and identification of deletions in the viral genome that encode proteins involved in interferon antagonism. Viruses. 2020, 12:1164; doi:10.3390/v**.
24. Chunyan Wu, Bingjun Shi, Di Yang, Kun Zhang, Jie Li, Jie Wang, Hongliang Liu, Qin Zhao, En-Min Zhou*, and Yuhen Nan*.Porcine Reproductive and Respiratory Syndrome Virus promotes SLA-DR-mediated antigen presentation of non-structure proteins to evoke a non-neutralizing antibody response in vivo. J Virol. 2020, 94:21. e01423-20.
23. Yafei Li, Chunyan Wu, Junhui Li, Sun He, Wei Zhang, Jian Cao, Xiaomei Pan, Huifen Tang, En-Min Zhou*, Yuchen Nan*. Interferon inducing Porcine Reproductive and Respiratory Syndrome Virus vaccine candidate protected piglets from HP-PRRSV challenge and evoke a higher level of neutralizing antibodies response in vivo. Vaccines. 2020.
22. Yafeng Hao, Taogeng Du, Gaoju Pang, Jiahua Li, Huizhuo Pan, Yingying Zhang, Lizhen Wang, Jin Chang, En-min Zhou*, and Hanjie Wang*. Spatiotemporal regulation of ubiquitin-mediated protein degradation via upconversion optogenetic nanosystem. Nano Res. 2020, doi.org/10.1007/s12274-020-2998-z.
21. Teng Zhang, Yunchao Liu, Yumei Chen, Aiping Wang, Hua Feng, Qiang Wei, Enmin Zhou, Gaiping Zhang*. A single dose glycoprotein D-based subunit vaccine against pseudorabies virus infection. Vaccine. 2020, 38:61853-6161.
20. Qizhong Lu, Xiaoxuan Li, Jiakai Zhao, Jiahong Zhu, yuhang Luo, Hong Duan, Pinpin Ji, Kun Wang, Baoyuan Liu, Xueting Wang, Wenqi Fan, Yani Sun, En-Min Zhou* and Qin Zhao*. Nonobody-horseradish peroxidase and –EGFP fusions as reagents to detect porcine parvovirus in the immunoassyas. J Nanobiotechnol. 2020, 18:7. Doi.org/10.1186/s12951-019-05698-x.
19. Yiyang Chen, Tianxiang Chen, Yuhang Luo, Jie Fan, Meimei Zhang, Qin Zhao, Yuchen Nan, Baoyuan Liu*, En-Min Zhou*.Synthetic peptides containing three neutralizing epitopes of genotype 4 swine hepatitis E virus (HEV) ORF2 induced protection against swine HEV infection in rabbit. Vaccines. 2020, 8:178; doi:10.3390/vaccines**..
18. Biyun Xue, Gaopeng Hou, Guixi Zhang, Jingjing Huang, Liangliang Li, Yuchen Nan, Yang Mu, Lizhen Wang, Lu Zhang, Ximeng Han, Xiaolei Ren, Qin Zhao, Chunyan Wu*, Jingfei Wang* and En-Min Zhou*. MYH9 Aggregation Induced by Direct Interaction With PRRSV GP5 Ectodomain Facilitates Viral Internalization by Permissive Cells. Frontiers Microbiol. 2019, 10:2313. doi: 10.3389/fmicb.2019.02313.
17. Liangliang Li, Lu Zhang, Qifan Hu, Liang Zhao, Yuchen Nan, Gaopeng Hou, Yiyang Chen, Ximeng Han, Xiaolei Ren, Qin Zhao, Hu Tao, Zhenzhao Sun, Gaiping Zhang, Chunyan Wu^*, Jingfei Wang^*, and En-Min Zhou^*. MYH9 Key Amino Acid Residues Identified by the Anti-Idiotypic Antibody to Porcine Reproductive and Respiratory Syndrome Virus Glycoprotein 5 Involve in the Virus Internalization by Porcine Alveolar Macrophages. Virueses. 2019 Dec 29;12(1). pii: E40. doi: 10.3390/v**.
16. Taofeng Du, Guang Zhu, Xiaoping Wu, Junyang Fang, En-Min Zhou. Biotinylated nanobody-based blocking ELISA for detection of antibodies against swine influenza virus. Int J Nanomedicine. 2019:14, 9337-9349..
15. Gaopeng Hou, Biyun Xue, Liangliang Li, Yuchen Nan, Lu Zhang, Kuokuo Li, Qin Zhao, Julian Hiscox, James Stewart, Chunyan Wu*, Jingfei Wang*, En-Min Zhou*. Direct interaction between CD163 N-terminal domain and MYH9 C-terminal domain contributes to porcine reproductive and respiratory syndrome virus internalization by permissive cells. Frontiers in Microbiol. 2019, 10: 1851. Doi: 10.3389/fmicb.2019.01851.
14. Huixia Li, Mengnan Fan, Baoyuan Liu, Pinpin Ji, Yiyang Chen, Beibei Zhang, Yani Sun, Baicheng Huang, Yuchen Nan, Zhenzhao Sun, James P. Stewart, Julian A. Hiscox, Qin Zhao*, En-Min Zhou*. Chicken organic anion transporting polypeptide 1A2, a novel avian hepatitis E virus (HEV) ORF2-interacting protein, is involved in avian HEV infection. J Virol. 2019, 93:11. DOI. 10.1128/JVI.02205-18.
13. Lizhen Wang^§, Lu Zhang^§, Baichen Huang^§, Kuokuo Li, Gaopeng Hou, Qin Zhao, Chunyan Wu, Yuchen Nan, Taofeng Du, Yang Mu, Jixun Lan, Hongying Chen^#, En-Min Zhou^#. A Nanobody Targeting Viral Non-Structural Protein 9 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication. J Virol. 2019 Feb 5;93(4). pii: e01888-18. doi: 10.1128/JVI.01888-18.
12. Huixia Li, Junyuan Wu, Yamin Sheng, Qizhong Lu, Baoyuan Liu, Yiyang Chen, Yani Sun, En-Min Zhou*, Qin Zhao*. Prevalence of hepatitis E virus (HEV) infection in various pig farms from Shaanxi Province, China: First detection of HEV RNA in pig semen. Transbound Emerg Dis. 2018 Jan;66(1):72-82.. DOI: 10.1111/tbed.12966.
11. Liangliang Li, Biyun Xue, Weiyao Sun, Guoqian Gu, Gaopeng Hou, Lu Zhang, Chunyan Wu, Qin Zhao,Yanjin Zhang, Gaiping Zhang, Julian A. Hiscox, Yuchen Nan* and En-Min Zhou*. Recombinant MYH9 Protein C-Terminal Domain Blocks Porcine Reproductive and Respiratory Syndrome Virus Internalization by Direct Interaction with Viral Glycoprotein 5. Antiviral Res. 2018 Aug;156:10-20. doi: 10.1016/j.antiviral.2018.06.001.
10. Yiyang Chen, Baoyuan Liu, Yani Sun, Huixia Li, Taofeng Du, Yuchen Nan, Julian A. Hiscox, En-Min Zhou*, Qin Zhao*. Characterization of Three Novel Linear Neutralizing B-cell Epitopes in the Capsid Protein of Swine Hepatitis E Virus. J Virol., 2018, 92(13): e00251-18. doi: 10.1128/JVI.00251-18.
9. Baoyuan Liu, Mengnan Fan, Beibei Zhang, Yiyang Chen, Yani Sun, Taofeng Du, Yuchen Nan, En-Min Zhou* and Qin Zhao*. Avian hepatitis E virus infection of duck, goose, and rabbit in northwest of China. Emerging Microbes & Infections. 2018, 7(1):76. doi: 10.1038/s41426-018-0075-4.
8. Yuchen Nan, Chunyan Wu, Qin Zhao, En-Min Zhou*. Zoonotic hepatitis E virus: an ignored risk for public health. Frontiers in Microbiol. 2017, 8:2396. doi:
7 Yuchen Nan, Chunyan Wu, Guoqian Gu, Weiyao Sun, Yan-Jin Zhang^* and En-Min Zhou^*. Improved Vaccine against PRRSV: Current progress and future perspective. Frontiers in Microbiol. 2017, 8:1635. doi: 10.3389/fmicb.2017.01635. eCollection 2017.
6. Taofeng Du, Yuchen Nan, Shuqi Xiao, Qin Zhao, and En-Min Zhou^*. Antiviral Strategies against PRRSV Infection. Trends in Microbiol. 2017: 25(12), 968-979. doi:10.1016/j.tim.2017.06.001.
5. Shahid Faraz Syed, Yani Sun, Taofeng Du, Yiyang Chen, Baoyuan Liu, Xinjie Wang, Huixia Li, Yuchen Nan, En-Min Zhou^*, Qin Zhao^*. Evaluation of recombinant Chinese avian hepatitis E virus (CaHEV) ORF2 and ORF3 proteins for protection of chickens against CaHEV infection. Vaccine. 2017, 35(27):3482-3489. doi: 10.1016/j.vaccine.2017.05.030.
4. Angke Zhang, Lijuan Zhao, Na Li, Hong Duan, Hongliang Liu, Fengxing Pu, Gaiping Zhang, En-Min Zhou^*, Shuqi Xiao^*. Carbon Monoxide Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by the cGMP/PKG and NF-κB Signaling Pathway. J Virol. 2016 Dec 16;91(1). pii: e01866-16.
3. Na Li, Taofeng Du, Yunhuan Yan, Angke Zhang, Jiming Gao, Gaopeng Hou, Shuqi Xiao*, En-Min Zhou*. MicroRNA let-7f-5p Inhibits Porcine Reproductive and Respiratory Syndrome Virus by Targeting MYH9. Sci Reports. 2016 Sep 30;6:34332. doi: 10.1038/srep34332.
2. Yongkun Du, Taofeng Du, Yunpeng Shi, Angke Zhang, Chong Zhang, Yuwen Diao, Guangyi Jin*, En-Min Zhou*. Synthetic Toll-like receptor 7 ligand inhibits porcine reproductive and respiratory syndrome virus infection in primary porcine alveolar macrophages. Antiviral Res. 2016, 131:9-18 doi:10.1016/j.antiviral.2016.04.005.
1. Jiming Gao, Shuqi Xiao, Yihong Xiao, Xiangpeng Wang, Chong Zhang, Qin Zhao, Baichen Huang, Hongliang Liu, Chengbao Wang, Ningning Liu, Junhua Lv, Taofeng Du, Yani Sun, Yang Mu, Gang Wang, Shahid Faraz Syed, Gaiping Zhang, Julian A. Hiscox, Ian Goodfellow and En-Min Zhou*. MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection. Sci Reports, 2016 Apr 26; 6:25120. doi: 10.1038/srep25120.