个人简介

任生物工程学会糖生物学工程分会副主任；中国生物工程学会青年委员会委员；中国细胞生物学青年委员会委员；生物物理学会糖生物学分会理事；美国糖生物学学会会员；生物学杂志编委，西北大学生命科学学院副院长
通信地址：西北大学生命科学学院，西安市太白北路229号，邮编：710069
教育经历
1997-2001 西北大学生命科学学院 生物化学专业 本科
2001-2007 中国农业大学微生物学专业 博士 导师：李季伦 中科院院士
2007-2011 美国华盛顿大学病理学系 博士后 导师：SI Hakomori 美国科学院院士
2011-2016 江南大学生物工程学院 教授，博士生导师
2017-至今 西北大学生命科学学院 教授，博士生导师
主要研究方向：肿瘤糖生物学
组成生命的基本物质包括：4种核苷酸、20种基本氨基酸、8种脂肪酸和32单糖。细胞中具有生物学功能的糖以糖链形式存在，糖链与蛋白质或脂类相结合形成糖缀合物，包括糖鞘脂、糖蛋白、GPI锚定蛋白和蛋白聚糖等。生物体内超过50%的蛋白质是被糖基化修饰的，糖链在生命活动中不可或缺，参与了包括蛋白质折叠、运输和清除、受体活化、信号转导、细胞内吞、细胞黏附和肿瘤迁移等生物学过程。因此，利用多组学技术（蛋白质组学、糖组学和基因组学等）寻找（肿瘤）生物学过程中的特异性糖链，系统地阐释糖链的生物学功能及其调控的分子机制；研究生物学过程中糖链、糖基转移酶和相关基因的分子靶标变化；以期为肿瘤特性的分子机制诠释和靶向治疗奠定基础。
承担的科研项目

1）国家自然科学基金面上项目，2020-2023，主持，在研
2）陕西省高校青年创新团队项目，2019-2021，主持，在研
3）科技部重点研发计划子课题，2018-2020，主持，在研
4）陕西省自然科学基金重点项目，2019-2021，主持，在研
5）国家自然科学基金面上项目，2017-2020，主持，在研
6）江苏省自然科学基金面上项目，2016-2019，主持，已结题
7）国家自然科学基金青年项目，2013-2015，主持，已结题
8）江苏省自然科学基金青年项目，2013-2016，主持，已结题
9）中央高校基本科研业务费专项重点项目，2014-2016，主持，已结题
10）江苏省“双创人才”项目，2014-2016，主持，已结题
11）江苏省双创团队项目，2013-2015，参与，已结题
主持其他企业横向科研项目5项
主要研究成果

代表性论文：(*通讯作者)

2020年
1. Zhou X , Zhang J , Song Z , Lu S , Yu Y , Tian J , Li X , Guan F* . ExoTracker: a low-pH-activatable fluorescent probe for labeling exosomes and monitoring endocytosis and trafficking. Chem Commun (Camb). 2020 Nov 26;56(94):14869-14872. doi: 10.1039/d0cc06208a.
2. Z Tan, L Cao, Y Wu, B Wang, Z Song, J Yang, L Cheng, X Yang, X Zhou, Z Dai*, X Li*, F Guan*. Bisecting GlcNAc modification diminishes the pro-metastatic functions of small extracellular vesicles from breast cancer cells。J Extracell Vesicles. 2020;10:56-72.
3. Li H, Wang Y, Pang X, Xie C, Deeg HJ, Wang H, Wan T, Wu J, Guan F*, Li X*.Elevated TWIST1 expression in myelodysplastic syndromes/ acute myeloid leukemia reduces efficacy of hypomethylating therapy with decitabine. Haematologica. Haematologica. 2020 Jan 30;105(10):235325. doi: 10.3324/haematol.2019.235325.
4. Zhou X, Yang G*, Guan F*. Biological Functions and Analytical Strategies of Sialic Acids in Tumor.Cells. 2020 Jan 22;9(2). pii: E273. doi: 10.3390/cells**.
5. Xiaoman Zhou; Yanhong Zhai; Changmei Liu; Ganglong Yang; Jia Guo; Chengwen Sun; Guang Li; Xiaowei Qi; Xiang Li; Feng Guan*. Sialidase NEU1 suppresses progression of human bladder cancer cells by inhibiting fibronectin-integrin α5β1 interaction and Akt signaling pathway. Cell Communication and Signaling. 2020 Mar 12;18(1):44. 视频链接：https://www.researchsquare.com/article/rs-25849/v1
6. Qiushi Chen, Zengqi Tan, Feng Guan*, Yan Ren*.The Essence of Functions and Detection of Bisecting GlcNAc in Cell Biology. Front. Chem. - Chemical Biology, 2020 May, DOI: 10.3389/fchem.2020.00511
7. Cheng L, Cao L, Wu Y, Xie W, Li J, Guan F, Tan Z. Bisecting N-Acetylglucosamine on EGFR Inhibits Malignant Phenotype of Breast Cancer via Down-Regulation of EGFR/Erk Signaling. Front Oncol. 2020 Jun 16;10:929. doi: 10.3389/fonc.2020.00929. eCollection 2020.
2019年
1. Xiaoman Zhou, Shang Yang, Ganglong Yang, Zengqi Tan, Feng Guan*. Two-step derivatization and mass spectral distinction of α2, 3 and α2, 6 sialic acid linkages on N-glycans by MALDI-TOF. Chinese Chemical Letter. 2019,30(3): 676-680
2. Dinghao Zhuo, Feng Guan*. Ganglioside GM1 promotes contact inhibition of growth by regulating the localization of epidermal growth factor receptor from glycosphingolipids-enriched microdomain to caveolae. Cell proliferation. 2019 Jul;52(4):e12639. doi: 10.1111/cpr.12639.
3. Bowen Wang, Zengqi Tan and Feng Guan*.Tumor-Derived Exosomes Mediate the Instability of Cadherins and Promote Tumor Progression. Int. J. Mol. Sci. 2019, 20, 3652; doi:10.3390/ijms**
2018年
1. Zengqi Tan, Chenxing Wang, Xiang Li and Feng Guan*. Bisecting N-acetylglucosamine structures inhibit hypoxia-induced epithelial-mesenchymal transition in breast cancer cells. Front. Physiol. 2018. doi: 10.3389/fphys.2018.00210
2. Jin ZC, Kitajima T, Dong W, Huang YF, Ren WW, Guan F, Chiba Y, Gao XD, Fujita M.Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans. J Biol Chem. 2018 Feb 23. pii: jbc.M117.813030. doi: 10.1074/jbc.M117.813030.
3. Pang X, Li H, Guan F, Li X. Multiple Roles of Glycans in Hematological Malignancies. Front Oncol. 2018 Sep 6;8:364. doi: 10.3389/fonc.2018.00364. eCollection 2018.
4. Zhuo D, Li X, Guan F. Biological Roles of Aberrantly Expressed Glycosphingolipids and Related Enzymes in Human Cancer Development and Progression. Front Physiol. 2018 May 3;9:466. doi: 10.3389/fphys.2018.00466. eCollection 2018.
5. Guo D, Guo J, Li X, Guan F.Enhanced motility and proliferation by miR-10b/FUT8/p-AKT axis in breast cancer cells. Oncol Lett. 2018 Aug;16(2):2097-2104. doi: 10.3892/ol.2018.8891.
6. Pang X, Wang Y, Zhang S, Tan Z, Guo J, Guan F, Li X. Altered susceptibility to apoptosis and N glycan profiles of hematopoietic KG1a cells following co culture with bone marrow derived stromal cells under hypoxic conditions. Oncol Rep. 2018 Sep; 40(3):1477-1486. doi: 10.3892/or.2018.6548
2017年
1. Guo J, Liu C, Zhou X, Xu X, Deng L, Li X, Guan F*. Conditioned Medium from Malignant Breast Cancer Cells Induces an EMT-Like Phenotype and an Altered N-Glycan Profile in Normal Epithelial MCF10A Cells. Int J Mol Sci. 2017 Aug 1;18(8). pii: E1528.
2. Guo Dong, Jia Guo, Xiang Li, and Feng Guan*. Differential effects of Pax3 on expression of polysialyltransferases STX and PST in TGF-β-treated normal murine mammary gland cells. Experimental Biology and Medicine. 2017, 242(2):177-183.
3. Yanli Wu, Ganglong Yang, Mingyong Miao, Feng Guan*. Enrichment and Characterization of Total O-linked Glycans from Glycoproteins by Ultrafiltration Units and Mass Spectrometry, Progress in Biochemistry and Biophysics, 2017; 44 (1)
4. Tian Xiang, Ganglong Yang, Xiaoyu Liu, Yidan zhou, Zhongxiao Fu, Fangfang Lu, Jianguo Gu, Naoyuki Taniguchi, Zengqi Tan, Xi Chen, Yan Xie, Feng Guan*, Xiao-Lian Zhang*. Alteration of N-glycan expression profile and glycan pattern of glycoproteins in human hepatoma cells after HCV infection. BBA-General Subjects, 2017, 1861:1036-1045.
2016年
1. Changmei Liu, Xiang Li and Feng Guan*. Progress of Neu1 and Related Signaling Pathway. Progress in Biochemistry and Biophysics, 2016, 10: 962-969.
2. Ganglong Yang, Luyu Huang, Jiaxu Zhang, Hanjie Yu, Zheng Li, Feng Guan*. Global Identification and Differential Distribution Analysis of Glycans in Subcellular Fractions of Bladder Cells. International Journal of Biological Sciences. 2016. 12(7):799-811.
3. Xiang Li, Xin Wang, Zengqi Tan, Si Chen, Feng Guan*. Role of Glycans in Cancer Cells Undergoing Epithelial–Mesenchymal Transition. Frontiers in Oncology. 2016; 6:33.
4. Ganglong Yang, Wei Lu, Di Yu, Chengwen Sun, Jia Guo, Zheng Li, Feng Guan*. Quantitative analysis of differential proteome expression in epithelial-to-mesenchymal transition of bladder epithelial cells using SILAC method. Molecules 2016, 21(1), 84
5. Yan-Hong Zhai, Chang-Mei Liu, Hui Guo, Gang-Long Yang, Feng Guan*. Effect of The Abnormal Expression of Sialidase Neu1 in Bladder Cancer on Toll Like Receptors. Progress in Biochemistry and Biophysics. 2016, 43(1): 55~62.
6. Shiwei Li, Changmei Liu, Jia Guo, Marcondes, A. M., Deeg, J., Xiang Li, Feng Guan*.. Iron overload induced by ferric ammonium citrate triggers reactive oxygen species-mediated apoptosis via both extrinsic and intrinsic pathways in human hepatic cells. Human & experimental toxicology, 2016: 35(6):598-607
7. Xin Wang, Xiang Li, Ying-Nan Zeng, Fa He, Xiao-Min Yang, Feng Guan*. Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples. International Journal of Molecular Medicine, 2016, 37(1): 197-206.
2015年
1. Feng Guan, Xiang Li, Jia Guo, Ganglong Yang, Xiang Li*. Ganglioside-magnetosome complex formation enhances uptake of gangliosides by cells. International Journal of Nanomedicine 2015:10: 6919-30.
2. Feng Guan*, Zengqi Tan, Xiang Li, Xingchen Pang, Yunlin Zhu, Dongliang Li, Ganglong Yang. A lectin-based isolation/ enrichment strategy for improved coverage of N-glycan analysis. Carbohydrate Research, 2015.416: 7-13.
3. Wei Lu, Gang-Long Yang, Peng Xue, Chang-Mei Liu, Feng Guan*. Quantitative Analysis of Integrin α6 in Bladder Cancer Cell Lines. Progress in Biochemistry and Biophysics, 2015, 42(5): 468~475
4. Feng Guan*, Xin Wang, Fa He. Promotion of Cell Migration by Neural Cell Adhesion Molecule (NCAM) Is Enhanced by PSA in a Polysialyltransferase-Specific Manner. PLOS ONE, 2015.
5. Ganglong Yang, Zhipeng Xu, Wei Lu, Xiang Li, Chengwen Sun, Jia Guo, Peng Xue , Feng Guan*. Quantitative Analysis of Differential Proteome Expression in Bladder Cancer vs. Normal Bladder Cells Using SILAC Method. PLOS ONE, 2015.
6. Xiang Li, Dongliang Li, Xingchen Pang, Ganglong Yang, H. Joachim Deeg, Feng Guan*. Quantitative Analysis of Glycans, Related Genes, and Proteins in Two Human Bone Marrow Stromal Cell Lines using an Integrated Strategy. Experimental Hematology, 2015.
7. Jia Guo, Yicheng Wang, Bo Song, Xin Wang, Ganglong Yang, Feng Guan*. Identification and functional characterization of intracellular sialidase NeuA3 from Streptomyces avermitilis. Process Biochemistry, 2015.2015, 50(5): 752-758.
8. Jia Guo, Bo Song, Xiang Li, Chenhui He, Ganglong Yang, Feng Guan*. Down-regulation of gangliotetraosylceramide (Gg4) and β3GalT4 gene expression by Smads during TGF-β-induced epithelial-mesenchymal transition. Molecular Medicine Reports. 2015, 11(3): 2241-2247.
2014年以前
1. Ganglong Yang, Zengqi Tan, Wei Lu, Jia Guo, Hanjie Yu, Jingmin Yu, Chengwen Sun, Xiaowei Qi, Zheng Li*, Feng Guan*. Quantitative glycome analysis of N-glycan patterns in bladder cancer vs. normal bladder cells using an integrated strategy. Journal of proteome research. 2015,14, 639-653.
2. Jia Guo, Xiang Li, Zengqi Tan, Wei Lu, Ganglong Yang, Feng Guan*. Alteration of N-glycans and Expression of Their Related Glycogenes in the Epithelial-Mesenchymal Transition of HCV29 Bladder Epithelial Cells. Molecules. 2014, 19(12), 20073-20090.
3. Zengqi Tan, Wei Lu, Xiang Li, Ganglong Yang, Jia Guo, Hanjie Yu, Zheng Li, Feng Guan*. Altered N-glycan expression profile in epithelial-to-mesenchymal transition of NMuMG cells revealed by an integrated strategy using mass spectrometry and glycogene and lectin microarray analysis. J Proteome Res. 2014, 13 (6), 2783–2795
4. Feng Guan*, Kazuko Handa, Sen-itiroh Hakomori. Regulation of epidermal growth factor receptor through interaction of ganglioside GM3 with GlcNAc of N-linked glycan of the receptor: Demonstration in ldlD cells. Neurochemical Research. 2011, 36(9) 1645-1653.
5. Feng Guan, Lana Schaffer, Kazuko Handa, Sen-itiroh Hakomori. Functional Role of Gangliotetraosylceramide in Epithelial-to-Mesenchymal Transition Process Induced by Hypoxia and by TGFβ. The FASEB Journal 2010, 24 (12) 4889-4903.
6. Feng Guan, Kazuko Handa and Sen-itiroh Hakomori. Specific glycosphingolipids mediate epithelial-to-mesenchymal transition of human and mouse epithelial cell lines. Proceedings of the National Academy of Sciences. 2009, 106 (18) 7461-7466.