其他题名Iron and manganese oxides enhance electron output efficiency of Clostridium pasteurianum
刘进超1,2; 刘芳华1
; 张月超1,2; 孟德龙3; 王学明3; 肖雷雷1发表期刊微生物学通报
ISSN0253-2654
2020
卷号47期号:1页码:24-34
关键词巴斯德梭菌电子输出率电化学活性铁锰氧化物异化金属还原发酵
研究领域Microbiology
英文摘要[Background] Fermentative dissimilatory metal-reducing bacteria, which are capable of reducing metallic oxides, get energy from fermentation. Little is known about how metallic oxides affect electron output efficiency of fermentative dissimilatory metal-reducing microorganisms. [Objective] This study was conducted to explore the influence of iron and manganese oxides (Fe_2O_3/MNO_2) on electron output efficiency. [Methods] Different concentrations of Fe_2O_3/MNO_2 were added into fermented system containing glucose and inoculated 5% C. pasteurianum. Electrochemical activity of C. pasteurianum was detected. The concentrations of Fe(Ⅱ) and Mn(Ⅱ) were measured by ferrozine spectrophotometry and formaldoxime method. Fermentation substrate and metabolites of C. pasteurianum were detected by gas chromatography and high performance liquid chromatography. Lastly, we calculated the electron output efficiency. [Results] The current density peaked with the value of about 0.93 mA/m~2. The concentrations of Fe(Ⅱ) and Mn(Ⅱ) gradually accumulated. The consumption of glucose was increased by 9.4%/7.7%, Meanwhile, acetate production was increased by 37.5%/25.0%, and butyrate production was increased by 22.7%/6.8%. Additionally, hydrogen production was increased by 21.6%/9.8%, and the total electron output efficiency was increased by 24.27%/10.82%, respectively. The pH values between experimental group and control are no significant difference. [Conclusion] This study shows that iron and manganese oxides can improve the electron output efficiency of C. pasteurianum by increasing glucose consumption and buffering pH value. The results provide evidence for revealing the effects of multivalent metal oxides on the electron output of fermentative dissimilatory metal-reducing bacteria, and further expand our understanding of the interaction mechanism between multivalent metal oxides and fermentative dissimilatory metal-reducing bacteria.
中文摘要【背景】发酵型异化金属还原菌通过发酵获取能量,同时也具有一定的异化还原变价金属氧化物的能力,关于变价金属氧化物对发酵型异化金属还原菌电子输出率的影响还知之甚少。【目的】探究铁锰氧化物(Fe_2O_3/MNO_2)对发酵型异化金属还原菌Clostridium pasteurianum电子输出率的影响。【方法】将不同浓度Fe_2O_3/MNO_2添加到以葡萄糖为底物并接种5% C. pasteurianum的发酵体系中,利用电化学工作站检测C. pasteurianum电化学特性;以菲啰嗪(Ferrozine)显色法和甲醛肟法分别测定发酵体系中Fe(Ⅱ)、Mn(Ⅱ)含量;气相色谱、高效液相色谱检测发酵底物葡萄糖及代谢产物(乙酸、丁酸、CO_2和H2)随发酵时间的变化情况;最后计算发酵过程的电子输出率。【结果】研究表明,接种C. pasteurianum的微生物燃料电池可以检测到电流的产生,最大电流密度为0.93 mA/m~2;随着发酵时间的推移,反应体系中Fe(Ⅱ)和Mn(Ⅱ)的浓度逐渐增高;Fe_2O_3/MNO_2的添加使发酵体系中葡萄糖消耗量提高了9.4%/7.7%,同时,乙酸产量提高了37.5%/25.0%,丁酸产量提高了22.7%/6.8%,氢气产量提高了21.6%/9.8%,而总的电子输出率则提高了24.27%/10.82%;添加铁锰氧化物的实验组pH值与对照组相比无显著差异。【结论】铁锰氧化物的添加可以提高C. pasteurianum的电子输出率,其原因可能是增加了葡萄糖消耗和缓冲pH值。研究结果为揭示变价金属氧化物影响发酵型异化金属还原菌电子输出的规律提供了证据,并进一步拓展了对变价金属氧化物与发酵型异化金属还原菌之间相互作用机制的认识。
文章类型Article
资助机构国家自然科学基金; 中国科学院科学号高端用户项目; 山东省自然科学****基金; 泰山****青年专家计划
收录类别CSCD
语种中文
CSCD记录号CSCD:6673572
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30380
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室,山东烟台264003;
2.中国科学院大学,北京100049;
3.中国农业大学烟台研究院,山东烟台264670
推荐引用方式
GB/T 7714刘进超,刘芳华,张月超,等. 铁锰氧化物提高巴斯德梭菌电子输出率[J]. 微生物学通报,2020,47(1):24-34.
APA刘进超,刘芳华,张月超,孟德龙,王学明,&肖雷雷.(2020).铁锰氧化物提高巴斯德梭菌电子输出率.微生物学通报,47(1),24-34.
MLA刘进超,et al."铁锰氧化物提高巴斯德梭菌电子输出率".微生物学通报 47.1(2020):24-34.
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