其他题名Screening of plant growth promoting and salt tolerant rhizobacteria in Sesbania cannabina
王艳霞1,2,3; 解志红1,3
; 张蕾1,3; 常大勇4发表期刊微生物学报
ISSN0001-6209
2020
卷号60期号:5页码:1023-1035
关键词植物根际促生菌田菁耐盐
研究领域Agriculture
英文摘要[Objective] To explore the effects of halophyte Sesbania cannabina and its rhizosphere functional microorganisms on ameliorating saline alkali soil, we isolated and identified the plant growth promoting rhizobacteria (PGPR) of S. cannabina grew in saline-alkali soil of Yellow River Delta, and further studied the growth-promoting and salt-tolerant characteristics of PGPRs. [Methods] Nitrogen-fixing, phosphorus-dissolving and potassium-dissolving bacteria were isolated from rhizosphere soil of S. cannabina by selective culture and identified by 16S rRNA molecular biology. Traits of plant growth-promoting and salt tolerance of the strains were determined and analyzed, and strains with excellent traits were selected to further study their growth promotion effect on corn. [Results] We totally obtained 105 rhizosphere isolates, of which N102 had growth-promoting and salt tolerance activities. As shown by seed germination test, N102 could significantly increase the germination rate (47%, P<0.05), bud length (48.5%, P<0.05), root length (60%, P<0.05) of S. cannabina. The pot experiment also shows that N102 could significantly improve plant height, root length, shoot dry weight, root dry weight and chlorophyll content of corn under salt stress. According to phylogenetic analysis, the similarity between N102 and Enterobacter soli ATCC BAA-2102 (NR117547) sequence was 99.30%, this it was identified as Enterobacter genus. [Conclusion] Strain N102 has high salt-tolerant and plant growth-promoting characteristics, promising to develop as microbial inoculants or microbial fertilizers that can effectively promote the growth of crops in saline-alkali soils.
中文摘要【目的】为探究盐生植物田菁及其根际功能微生物改良盐碱地的效果,本研究从黄河三角洲盐碱区田菁根际土壤中分离促生菌,并明确其耐盐促生效果。【方法】采用选择培养方法从田菁根际土壤中分离固氮菌、解磷菌以及解钾菌,并进行16S rRNA分子生物学鉴定。之后对菌株的耐盐及促生特性进行测定,筛选性状优良菌株进行玉米促生作用研究。【结果】共分离得到105株根际促生菌,其中N102兼具多种促生特性且耐盐性达15%。田菁种子发芽试验表明,N102可显著提高田菁发芽率(47%, P<0.05)、芽长(48.5%,P<0.05)和根长(60%,P<0.05);玉米盆栽试验结果表明,N102对盐胁迫下玉米的株高、根长、叶绿素含量、地上部干重以及根干重具有显著的促进作用。经系统发育分析,N102与Enterobacter soli ATCC BAA-2102 (NR117547)序列相似度为99.30%,鉴定属于Enterobacter属。【结论】菌株N102具有多种植物促生耐盐特性,具有开发成有效促进盐碱地作物生长的微生物肥的良好前景。
文章类型Article
资助机构NSFC-山东省联合基金重点项目; 山东省重点研发计划; 边际土地产能效益扩增机理与藏粮于地技术模式
收录类别CSCD
语种中文
CSCD记录号CSCD:6699421
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30365
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.中国科学院烟台海岸带研究所,山东烟台264003;
2.中国科学院大学,北京100049;
3.中国科学院海洋大科学研究中心,山东青岛266071;
4.烟台固特丽生物科技股份有限公司,山东烟台264003
推荐引用方式
GB/T 7714王艳霞,解志红,张蕾,等. 田菁根际促生菌的筛选及其促生耐盐效果[J]. 微生物学报,2020,60(5):1023-1035.
APA王艳霞,解志红,张蕾,&常大勇.(2020).田菁根际促生菌的筛选及其促生耐盐效果.微生物学报,60(5),1023-1035.
MLA王艳霞,et al."田菁根际促生菌的筛选及其促生耐盐效果".微生物学报 60.5(2020):1023-1035.
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