Bi, Liyan1; Wang, Xiao1; Cao, Xiaowei2; Liu, Luying1; Bai, Congcong1; Zheng, Qingyin1; Choo, Jaebum4; Chen, Lingxin1,3,5
发表期刊TALANTA
ISSN0039-9140
2020-12-01
卷号220页码:8
关键词Au@AgNRSERS nanotagAntibiotic susceptibility testingMinimum inhibitory concentrationPrincipal component analysis
DOI10.1016/j.talanta.2020.121397
通讯作者Bi, Liyan(liyan_bi@163.com); Choo, Jaebum(jbchoo@cau.ac.kr); Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要There is a challenge to obtain an ultrasensitive and rapid approach to detect bacteria and identify resistance. As a powerful bioanalytical tool, surface-enhanced Raman scattering (SERS) in bacterial detection have attracted increasing attentions. Herein, we developed a SERS-active Au@Ag core-shell nanorod (Au@AgNR) tag platform for ultrasensitive bacteria detection and antibiotic-susceptibility testing (AST). The platform established that surface enhanced Raman scattered Rhodamine 6G (R6G) absorption at 1517 cm(-1) had a good linearity (RI = 3865 + 193logC; R-2 = 0.97) with logarithm of E. coli concentration over a range of 10(7)-10(2) CFU (colony forming unit)/mL with limit of detection as low 10(2) CFU/mL. When E. coli was exposed to ampicillin at minimum inhibitory concentration (MIC, 4 mu g/mL), Raman spectroscopy showed the obvious variation between ampicillin-susceptible E. coli (Amp(-)-E. coli) and the ampicillin-resistant E. coli (Amp(+)-E. coli). Combined with principal component analysis (PCA) statistical analysis, the Raman intensity variation mentioned above allows to obtain rapid antibiotic resistance testing (<3.5 h). In addition, E.coli spiked into blood from C57BL/6 mice can be identified clearly, indicating the potential for point-of-care diagnostics.
资助机构National Natural Science Foundation of China; National Research Foundation of Korea; Research Initiation Fund of Binzhou Medical University; Taishan Scholar Project Special Funding
收录类别SCI
语种英语
关键词[WOS]ENHANCED RAMAN-SCATTERING; SENSITIVE DETECTION; GOLD; IDENTIFICATION; SPECTROSCOPY; DESIGN; PROBE; ARRAY
研究领域[WOS]Chemistry
WOS记录号WOS:000571477400006
引用统计被引频次：20[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/28397
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
通讯作者Bi, Liyan; Choo, Jaebum; Chen, Lingxin作者单位1.Binzhou Med Univ, Transformat Otol & Neurosci Ctr, Yantai 264003, Peoples R China
2.Yangzhou Univ, Med Coll, Inst Translat Med, Yangzhou 225001, Jiangsu, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
4.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea
5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Bi, Liyan,Wang, Xiao,Cao, Xiaowei,et al. SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing[J]. TALANTA,2020,220:8.
APABi, Liyan.,Wang, Xiao.,Cao, Xiaowei.,Liu, Luying.,Bai, Congcong.,...&Chen, Lingxin.(2020).SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing.TALANTA,220,8.
MLABi, Liyan,et al."SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing".TALANTA 220(2020):8.
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