Li, Bowei1
; Qi, Ji1; Fu, Longwen1; Han, Jinglong2,3; Choo, Jaebum4; DeMello, Andrew J.5; Lin, Bingcheng6; Chen, Lingxin1,7发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
2020-10-01
卷号165页码:9
关键词Microfluidic paper-based devicemu PADOn site diagnosisCancer biomarker
DOI10.1016/j.bios.2020.112282
通讯作者Choo, Jaebum(jbchoo@cau.ac.kr); DeMello, Andrew J.(andrew.demello@chem.ethz.ch); Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要To date, 55% of inhabitants of the developing world still live in rural regions and they have a very urgent need to improve the level of disease diagnosis, especially in resource-limited settings. Herein, we present a microfluidic system that centrifuges whole blood and quantifies contained biomarkers in a rapid, feasible and integrated way. Such a device provides a blood-in/answer-out capability and is small enough to be carried by any individual in any environment. The successful integration a hand-powered centrifuge and immunoassay unit within a rotational paper-based device allows for diagnostic application by untrained users and in environments where access to electricity cannot be assumed. In addition, the low cost (similar to$ 0.5), light weight and small instrumental footprint make the device ideally suited for rapid on-site detection. To validate the applicability of the system in a clinical diagnostic testing, we successfully perform enzyme-linked immunosorbent assay (ELISA) analysis of carcinoembryonic antigen and alpha fetoprotein from human blood samples. We expect that this powerful platform technology will provide the opportunities for point-of-care diagnosis in resource-limited settings.
资助机构National Key Research and Development Program of China; National Natural Science Foundation of China; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences; government-wide R&D Fund for the Research of Infectious Diseases in Korea; National Research Foundation of Korea
收录类别SCI
语种英语
关键词[WOS]MICROFLUIDIC DEVICES; SEPARATION; IMMUNOSENSORS; IMMUNODEVICE; IMMUNOASSAY; PLASMA; ASSAYS; TIME
研究领域[WOS]Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics
WOS记录号WOS:000566444800001
引用统计被引频次：18[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/28375
专题中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者Choo, Jaebum; DeMello, Andrew J.; Chen, Lingxin作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
2.Yantai Univ, Sch Environm & Mat Engn, Yantai 264005, Peoples R China
3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China
4.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea
5.Swiss Fed Inst Technol, Dept Chem & Appl Biosci, Inst Chem & Bioengn, Vladimir Prelog Weg 1, CH-8093 Zurich, Switzerland
6.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
7.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Li, Bowei,Qi, Ji,Fu, Longwen,et al. Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities[J]. BIOSENSORS & BIOELECTRONICS,2020,165:9.
APALi, Bowei.,Qi, Ji.,Fu, Longwen.,Han, Jinglong.,Choo, Jaebum.,...&Chen, Lingxin.(2020).Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities.BIOSENSORS & BIOELECTRONICS,165,9.
MLALi, Bowei,et al."Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities".BIOSENSORS & BIOELECTRONICS 165(2020):9.
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