Lv, Enguang1,2,5; Li, Yanhong1,2,5; Ding, Jiawang1,2,3,4
; Qin, Wei1,2,3,4发表期刊ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN1433-7851
2020-11-27
页码6
关键词biosensorsmagnetic fieldspeptidespotentiometry
DOI10.1002/anie.202011331
通讯作者Ding, Jiawang(jwding@yic.ac.cn); Qin, Wei(wqin@yic.ac.cn)
英文摘要We report here the concept of a magnetically controlled extraction of hydrophilic bioreceptors into polymeric membranes for bioassays. The potentiometric assay relies on the intrinsic charges of an antimicrobial peptide and its unique recognition abilities, which can eliminate the probe labeling and indicator addition. The target binding event could effectively prevent the extraction of the peptide into the polymeric membrane doped with an ion exchanger, thus resulting in a potential change. The potentiometric response properties of the peptide assembled on magnetic beads can be dynamically controlled and modulated by applying a magnetic field. Staphylococcus aureus, as a model of food-borne pathogens, was measured at levels down to 10 CFU mL(-1). Based on this sensing strategy, a potentiometric array was developed for the pattern recognition of bacteria. The proposed general platform can be used for potentiometric biosensing using other hydrophilic bioreceptors.
资助机构National Natural Science Foundation of China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao); Instrument Developing Project of the Chinese Academy of Sciences; National Key Research and Development Program of China; Taishan Scholar Program of Shandong Province; Key Research and Development Program of Yantai
收录类别SCI
语种英语
关键词[WOS]ION-SELECTIVE ELECTRODES; PROTAMINE; PROTEINS; DESIGN
研究领域[WOS]Chemistry
WOS记录号WOS:000592466700001
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/28312
专题中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
通讯作者Ding, Jiawang; Qin, Wei作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Lv, Enguang,Li, Yanhong,Ding, Jiawang,et al. Magnetic-Field-Driven Extraction of Bioreceptors into Polymeric Membranes for Label-Free Potentiometric Biosensing[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2020:6.
APALv, Enguang,Li, Yanhong,Ding, Jiawang,&Qin, Wei.(2020).Magnetic-Field-Driven Extraction of Bioreceptors into Polymeric Membranes for Label-Free Potentiometric Biosensing.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,6.
MLALv, Enguang,et al."Magnetic-Field-Driven Extraction of Bioreceptors into Polymeric Membranes for Label-Free Potentiometric Biosensing".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020):6.
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