其他题名Soil microbial community structure along the ecological succession in Yellow River Delta
党消消1; 张蕾1; 王伟4; 王光美1
; 刘润进5; 解志红1发表期刊微生物学报
ISSN0001-6209
2020
卷号60.0期号:006页码:1272-1283
关键词宏基因组测序微生物多样性盐生植物生态演替黄河三角洲
英文摘要【目的】黄河三角洲区域具有重要的湿地生态系统。碱蓬、野大豆和芦苇是该地区典型的盐生植物。本研究针对碱蓬、野大豆和芦苇混生植物的根际土壤微生物群落组成和功能基因进行了分析比较。【方法】对碱蓬,野大豆-芦苇混生植物的根际微生物菌群和光滩土壤菌群进行了宏基因组测序,使用COG和KEGG数据库对微生物菌群的功能进行了注释和比较。【结果】本研究结果表明,变形菌门是3个取样点的主要菌门,其在碱蓬、野大豆-芦苇根际土壤中的相对含量比光滩土壤分别多28.8%和10.6%。此外,拟杆菌门、放线菌门和芽单胞菌门是3个取样点中的优势物种。中华根瘤菌属是野大豆-芦苇混生植物根际土壤的最主要的属。对功能基因进行分析表明,光滩土壤中的功能基因的数量多于碱蓬根际土壤和野大豆-芦苇混生植物根际土壤的功能基因数。在这3个位点中,氨基酸代谢、碳水化合物代谢和能量代谢,以及无机离子转运和代谢的基因最多。【结论】本研究为发掘有价值的微生物资源和海岸带盐碱土壤修复提供了一定的理论基础。
中文摘要ObjectiveThe Yellow River Delta is one of the most important estuary wetland ecosystems in the world.Suaeda glauca(SG),Glycine soja and Phragmites australis(GP)are three typical plants that play important roles in the ecosystem of this region.In this work,we compared the microbial community composition and functional genes of the rhizosphere soils of these typical halophytes with those of bulk soil of the barren wetland(BW)along the saline gradient in the Yellow River Delta.MethodsMetagenome sequencing was used to analyze the composition and abundance of the microbial communities.COG(Clusters of Orthologous Groups)and KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway analyses were used to determine the functional gene annotation of the predicted genes.ResultsThe results demonstrated that Proteobacteria was the most abundant phylum in all samples.The relative abundance of Proteobacteria in the rhizosphere soils of SG and GP was 28.8%and 10.6%greater than that of the BW,respectively.In addition,Bacteroidetes,Actinobacteria and Gemmatimonadetes were detected as abundant species in the three samples.Sinorhizobium was an abundant species in the GP sample and may be responsible for nitrogen fixation in the rhizosphere soils.Sequence annotation indicated that the number of the predicted functional genes of BW was higher than that of SG and GP.Among them,amino acid metabolism,carbohydrate and energy metabolism,and inorganic ion transport and metabolism genes were abundant in the three samples.ConclusionThis study demonstrated that different plants and soils have a coordinated effect on the soil microbial communities of the Yellow River Delta,and provided valuable insights on microbial resilience and their potential application on bioremediation of the coastal zone saline-alkali soils.
收录类别CSCD
语种中文
CSCD记录号CSCD:6729043
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/28153
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.中国科学院烟台海岸带研究所
2.中国科学院大学
3.中国科学院海洋大科学研究中心
4.烟台大学
5.青岛农业大学
推荐引用方式
GB/T 7714党消消,张蕾,王伟,等. 黄河三角洲原生演替中土壤微生物群落结构分析[J]. 微生物学报,2020,60.0(006):1272-1283.
APA党消消,张蕾,王伟,王光美,刘润进,&解志红.(2020).黄河三角洲原生演替中土壤微生物群落结构分析.微生物学报,60.0(006),1272-1283.
MLA党消消,et al."黄河三角洲原生演替中土壤微生物群落结构分析".微生物学报 60.0.006(2020):1272-1283.
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