Liu, Xiaolin1,2; Liu, Yanan1,2; Johnson, Kevin Scot3; Dong, Xiaoyan1,4; Xie, Zhihong1,4,5
发表期刊FRONTIERS IN MICROBIOLOGY
ISSN1664-302X
2020-12-07
卷号11页码:12
关键词chemotaxisCheZcellular localizationAzorhizobium caulinodansrhizobia
DOI10.3389/fmicb.2020.585140
通讯作者Xie, Zhihong(zhxie@yic.ac.cn)
英文摘要Chemotaxis is essential for the competitiveness of motile bacteria in complex and harsh environments. The localization of chemotactic proteins in the cell is critical for coordinating a maximal response to chemotactic signals. One chemotaxis protein with a well-defined subcellular localization is the phosphatase CheZ. CheZ localizes to cell poles by binding with CheA in Escherichia coli and other enteric bacteria, or binding with a poorly understood protein called ChePep in epsilon-Proteobacteria. In alpha-Proteobacteria, CheZ lacks CheA-binding sites, and its cellular localization remains unknown. We therefore determined the localization of CheZ in the alpha-Proteobacteria Azorhizobium caulinodans ORS571. A. caulinodans CheZ, also termed as CheZ(AC,) was found to be located to cell poles independently of CheA, and we suspect that either the N-terminal helix or the four-helix bundle of CheZ(AC) is sufficient to locate to cell poles. We also found a novel motif, AXXFQ, which is adjacent to the phosphatase active motif DXXXQ, which effects the monopolar localization of CheZ(AC). This novel motif consisting of AXXFQ is conserved in CheZ and widely distributed among Proteobacteria. Finally, we found that the substitution of phosphatase active site affects the polar localization of CheZ(AC). In total, this work characterized the localization pattern of CheZ containing a novel motif, and we mapped the regions of CheZ(AC) that are critical for its polar localization.
资助机构NSFC-Shandong Joint Fund Key Projects; National Natural Science Foundation of China
收录类别SCI
语种英语
关键词[WOS]CHEMOTAXIS PHOSPHATASE CHEZ; ESCHERICHIA-COLI; POLAR LOCALIZATION; PHOSPHORYLATED CHEY; SIGNALING PROTEINS; COMPLEX; CHEMORECEPTOR; BACTERIA; BINDING; DIVERSITY
研究领域[WOS]Microbiology
WOS记录号WOS:000600397400001
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27559
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Xie, Zhihong作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China
2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China
3.Univ Calif Santa Cruz, Dept Microbiol & Environm Toxicol, Santa Cruz, CA 95064 USA
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
5.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Tai An, Shandong, Peoples R China
推荐引用方式
GB/T 7714Liu, Xiaolin,Liu, Yanan,Johnson, Kevin Scot,et al. Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571[J]. FRONTIERS IN MICROBIOLOGY,2020,11:12.
APALiu, Xiaolin,Liu, Yanan,Johnson, Kevin Scot,Dong, Xiaoyan,&Xie, Zhihong.(2020).Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571.FRONTIERS IN MICROBIOLOGY,11,12.
MLALiu, Xiaolin,et al."Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571".FRONTIERS IN MICROBIOLOGY 11(2020):12.
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