Xin, Zifan1; Yu, Daode6; Yang, Bowen1; Chen, Lizhu4; Hayouka, Zvi5; Chen, Xingfu1; Gong, Yitong1; Dai, Haiyu1; Wang, Lei1
; Zhao, Yancui1; Liu, Xiaoli1; Yang, Dinglong2,3发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2020-12-01
卷号107页码:260-268
关键词Venerupis philippinarumC-type lectinMolecular characterizationImmune recognition
DOI10.1016/j.fsi.2020.10.006
通讯作者Liu, Xiaoli(lxlshz2006@163.com); Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要In the present study, two C-type lectins (designated as VpClec-3 and VpClec-4) were identified and characterized from the manila clam Venerupis philippinarum. Multiple alignment and phylogenetic relationship analysis strongly suggested that VpClec-3 and VpClec-4 belong to the C-type lectin family. In nonstimulated clams, the VpClec-3 transcript was dominantly expressed in the hepatopancreas, while the VpClec-4 transcript was mainly expressed in gill tissues. Both VpClec-3 and VpClec-4 mRNA expression was significantly upregulated following Vibrio anguillarum challenge. Recombinant VpClec-4 (rVpClec-4) was shown to bind lipopolysaccharide (LPS) and glucan in vitro, whereas recombinant VpClec-3 (rVpClec-3) only bound to glucan. In addition, rVpClec-3 and rVpClec-4 displayed broad agglutination activities towards Vibrio harveyi, Vibrio splendidus and V. anguillarum, while no agglutination activities towards Enterobacter cloacae or Aeromonas hydrophila were observed in rVpClec-3. Moreover, hemocyte phagocytosis was significantly enhanced by rVpClec-3 and rVpClec-4. All the results showed that VpClecs function as pattern recognition receptors (PRRs) with distinct recognition spectra and are potentially involved in the innate immune responses of V. philippinarum.
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences; National Natural Science Foundation of China; Natural Science Foundation of Shandong Province; Yantai Science and Technology Development Project; Marine and Fisheries Science and Technology Innovation Program of Shandong
收录类别SCI
语种英语
关键词[WOS]RECOGNITION; CLONING; GENE; HEMOCYTES; IDENTIFICATION; DISEASE; CLAM
研究领域[WOS]Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS记录号WOS:000599882200029
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27553
专题支撑部门_中国科学院牟平海岸带环境综合试验站
中国科学院烟台海岸带研究所
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者Liu, Xiaoli; Yang, Dinglong作者单位1.Ludong Univ, Key Lab Marine Biotechnol Univ Shandong, Sch Life Sci, Yantai 264025, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
5.Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Inst Biochem Food Sci & Nutr, IL-76100 Rehovot, Israel
6.Marine Biol Inst Shandong Prov, Qingdao 266002, Shandong, Peoples R China
推荐引用方式
GB/T 7714Xin, Zifan,Yu, Daode,Yang, Bowen,et al. Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2020,107:260-268.
APAXin, Zifan.,Yu, Daode.,Yang, Bowen.,Chen, Lizhu.,Hayouka, Zvi.,...&Yang, Dinglong.(2020).Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,107,260-268.
MLAXin, Zifan,et al."Molecular characterization, expression and immune functions of two C-type lectin from Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 107(2020):260-268.
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