Sun, Yanyu1,2,3,4; Chen, Weiwei1,2,3,4; Wang, Yibo1,2,3,4; Guo, Jie1
; Zhang, Haikun1,2,3; Hu, Xiaoke1,2,3发表期刊JOURNAL OF ENVIRONMENTAL SCIENCES
ISSN1001-0742
2021-02-01
卷号100页码:317-327
关键词Crude oil bioaugmentationBacterial community dynamic shiftEnhanced green fluorescent protein
DOI10.1016/j.jes.2020.07.025
通讯作者Hu, Xiaoke(xkhu@yic.ac.cn)
英文摘要The biodegradation was considered as the prime mechanism of crude oil degradation. To validate the efficacy and survival of the crude oil-degrading strain in a bioremediation process, the enhanced green fluorescent protein gene (egfp) was introduced into Acinetobacter sp. HC8-3S. In this study, an oil-contaminated sediment microcosm was conducted to investigate the temporal dynamics of the physicochemical characterization and microbial community in response to bacterium amendment. The introduced strains were able to survive, flourish and degrade crude oil quickly in the early stage of the bioremediation. However, the high abundance cannot be maintained due to the ammonium (NH4+-N) and phosphorus (PO43--P) contents decreased rapidly after 15 days of remediation. The sediment microbial community changed considerably and reached relatively stable after nutrient depletion. Therefore, the addition of crude oil and degrading cells did not show a long-time impact on the original microbial communities, and sufficient nitrogen and phosphorus nutrients ensures the survive and activity of degrader. Our studies expand the understanding of the crude oil degradative processes, which will help to develop more rational bioremediation strategies. (C) 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
资助机构International Cooperation, Chinese Academy of Sciences, Chinese-foreign Cooperation in key projects The detection of oil spill and its ecological impact study; State's Key Project of Research and Development Plan
收录类别SCI
语种英语
关键词[WOS]CONTAMINATED SOIL; DEGRADATION; COMMUNITY; STRAIN; BIOREMEDIATION; IDENTIFICATION; BIODEGRADATION; BIOSTIMULATION; BACTERIA; REFINERY
研究领域[WOS]Environmental Sciences & Ecology
WOS记录号WOS:000596939800032
引用统计被引频次：3[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27526
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室
通讯作者Hu, Xiaoke作者单位1.Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Sun, Yanyu,Chen, Weiwei,Wang, Yibo,et al. Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2021,100:317-327.
APASun, Yanyu,Chen, Weiwei,Wang, Yibo,Guo, Jie,Zhang, Haikun,&Hu, Xiaoke.(2021).Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process.JOURNAL OF ENVIRONMENTAL SCIENCES,100,317-327.
MLASun, Yanyu,et al."Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process".JOURNAL OF ENVIRONMENTAL SCIENCES 100(2021):317-327.
PDF全文下载地址:
点我下载PDF
