Miao, Qin1,2; Cui, Yulin1,2
; Zhang, Jingjing1,2; Mi, Yingqi1,2,3; Tan, Wenqiang1,2; Li, Qing1,2; Gu, Guodong4; Dong, Fang1,2; Guo, Zhanyong1,2,3发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
2020-12-01
卷号164页码:384-388
关键词Ratio coefficientDetermination of chitosan contentHydrolysis rateHPLCAverage deacetylation degree (DD)
DOI10.1016/j.ijbiomac.2020.07.013
通讯作者Guo, Zhanyong(zhanyongguo@hotmail.com)
英文摘要The determination of chitosan is a significant topic that has been growing in importance for a variety of fields. To quantify chitosan, various analytical methods can be used. When high performance liquid chromatographic (HPLC) is used for quantitative analysis of chitosan, the chitosan is mainly hydrolyzed into glucosamine salts, which are determined by HPLC. Average deacetylation degree (DD) has an effect on acid hydrolysis of chitosan. In this paper, chitosan with different average DDs was hydrolyzed to observe its effect on the determination of chitosan content, and that the study of combining acid hydrolysis rates of chitosan (alpha) with different average DDs and chitin (beta) standard to form ratio coefficients (gamma) to quantify chitosan was designed. Then, the table about. values within certain average DD ranges of chitosan was obtained. By using. and a, the actual content of chitosan could be obtained simply and accurately. (C) 2020 Elsevier B.V. All rights reserved.
资助机构National Key Research and Development Programof China; Key Research and Development Program of Shandong Province; Natural Science Foundation of Shandong Province of China; Youth Innovation Promotion Association CAS
收录类别SCI
语种英语
关键词[WOS]ACID-HYDROLYSIS; LIQUID-CHROMATOGRAPHY; DEACETYLATION; GLUCOSAMINE; CHITIN; ACETYLATION
研究领域[WOS]Biochemistry & Molecular Biology; Chemistry; Polymer Science
WOS记录号WOS:000588093700035
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27523
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者Guo, Zhanyong作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Alliance Pharma Inc, 17 Lee Blvd, Malvern, PA 19355 USA
推荐引用方式
GB/T 7714Miao, Qin,Cui, Yulin,Zhang, Jingjing,et al. Determination of chitosan content with ratio coefficient method and HPLC[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,164:384-388.
APAMiao, Qin.,Cui, Yulin.,Zhang, Jingjing.,Mi, Yingqi.,Tan, Wenqiang.,...&Guo, Zhanyong.(2020).Determination of chitosan content with ratio coefficient method and HPLC.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,164,384-388.
MLAMiao, Qin,et al."Determination of chitosan content with ratio coefficient method and HPLC".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 164(2020):384-388.
PDF全文下载地址:
点我下载PDF
