Wang, Kang2; Gao, Zhengquan2; Wang, Yinchu1,3
; Meng, Chunxiao2; Li, Jialin1,3; Qin, Song1,3; Cui, Yulin1,3发表期刊ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
2021-04-01
卷号54页码:6
关键词Chlorella vulgarisChloroplastGenetic engineeringRecombinant proteinMicroparticle bombardment
DOI10.1016/j.algal.2021.102214
通讯作者Cui, Yulin(yulincui@yic.ac.cn)
英文摘要The purpose of this study was to establish a chloroplast transformation system in Chlorella vulgaris for expressing recombinant proteins. The 16S-trnI/trnA-23S in the inverted repeat region of C. vulgaris chloroplast genome was used as the flanking fragments in the homologous recombination vector. Meanwhile, the selectable marker aadA gene was promoted by the endogenous 16S promoter, and two codon-optimized antimicrobial peptide NZ2114 and Piscidin-4 genes linked by endogenous ribosome binding site (RBS) in a polycistron were regulated by the rbcL promoter of C. vulgaris. The plasmid was transformed into the chloroplast of C. vulgaris via microparticle bombardment and the transformants were screened using spectinomycin. After multiple rounds' selection, the homoplastic transformants were identified using polymerase chain reaction (PCR), Southern blotting, and Western blotting. This research provided an efficient chloroplast genetic system for C. vulgaris, and will be benefit for the algal further research and application.
资助机构National Key Research and Development Program of China; National Natural Science Foundation of China; Natural Science Foundation of Shandong Province, China; Project of Innovation & Development of Marine Economy; Major Basic Research Program of Shandong Province Natural Science Foundation; Key Research and Development Program of Shandong Province
收录类别SCI
语种英语
研究领域[WOS]Biotechnology & Applied Microbiology
WOS记录号WOS:000632293900009
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27327
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Cui, Yulin作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China
2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714Wang, Kang,Gao, Zhengquan,Wang, Yinchu,et al. The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,54:6.
APAWang, Kang.,Gao, Zhengquan.,Wang, Yinchu.,Meng, Chunxiao.,Li, Jialin.,...&Cui, Yulin.(2021).The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,54,6.
MLAWang, Kang,et al."The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 54(2021):6.
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