Li, Wenjun1,2
; Ma, Chengbo1,3; Ge, Baosheng4; Zang, Fan1,3; Qin, Song1,2; Lin, Jian3发表期刊JOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
2021-04-29
页码11
关键词Combinational biosynthesisPhycoerythrobilinResponse surface methodologyExtraction
DOI10.1007/s10811-021-02408-0
通讯作者Qin, Song(sqin@yic.ac.cn); Lin, Jian(linjian3384@163.com)
英文摘要Phycoerythrobilin (PEB) is a special pigment in red algae and cyanobacteria that plays an important role in capturing light energy. As a natural non-toxic pigment with antioxidant properties, PEB is widely used in food and cosmetics industries. At present, the presence of other pigments in red algae and cyanobacteria, such as chlorophyll and phycocyanobilin, makes the preparation of PEB complicated and costly. In this study, the PEB synthesizing enzyme genes of hox1 from Synechocystis sp. PCC6803 and pebS from Nostoc sp. PCC 7120 were co-expressed in Escherichia coli to produce recombinant PEB. In addition, single-factor and response surface methods were used to enhance the yield of recombinant PEB. Under the conditions of lactose concentration at 4 mmol L-1, induction temperature at 27 degrees C, OD600 nm of 0.9, and induction time at 14 h, the concentration of PEB reached 20.37 mg L-1. In addition, a high-purity (purity > 70%) PEB was obtained through chloroform extraction, and the purified product was verified in UV spectrometry, high-performance liquid chromatography, and mass spectrometry.
资助机构National Natural Science Foundation of China; Key Research and Development Program of Yantai City, Shandong, China
收录类别SCI
语种英语
研究领域[WOS]Biotechnology & Applied Microbiology; Marine & Freshwater Biology
WOS记录号WOS:000645498300002
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27224
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Qin, Song; Lin, Jian作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China
推荐引用方式
GB/T 7714Li, Wenjun,Ma, Chengbo,Ge, Baosheng,et al. Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli[J]. JOURNAL OF APPLIED PHYCOLOGY,2021:11.
APALi, Wenjun,Ma, Chengbo,Ge, Baosheng,Zang, Fan,Qin, Song,&Lin, Jian.(2021).Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli.JOURNAL OF APPLIED PHYCOLOGY,11.
MLALi, Wenjun,et al."Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli".JOURNAL OF APPLIED PHYCOLOGY (2021):11.
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