Li WJ(李文军)1
; Pu, Y3; Tang, ZH4; Zhao, FL2; Xie, MY2,5; Qin, S1发表期刊Chinese Journal of Physics
ISSN0577-9073
2020
卷号66页码:24-35
关键词B-phycoerythrinEnergy transferCircular dichroism spectraUltrafast spectroscopy
研究领域Physics
DOI10.1016/j.cjph.2020.03.025
通讯作者Xie, Mingyuan(xiemy5@mail.sysu.edu.cn); Qin, Song(sqin@yic.ac.cn)
英文摘要The B-phycoerythrin hexamer (αβ)6γ of Porphyridium purpureum was isolated and purified. The absorption, circular dichroism, fluorescence and ultrafast time-resolved spectra were obtained. The results showed a double absorption peak at 545 nm and 565 nm and a shoulder peak at 498 nm, and fluorescence emission maxima at 580 nm and 620 nm were observed. The circular dichroism spectra in the near-ultraviolet region were obtained and resolved for the first time, which showed that the two peaks at 260 nm and 305 nm were considered to be correlated to phenylalanine (Phe) and tryptophan (Trp) in a conservative hydrophobic microenvironment, respectively. The circular dichroism spectra in the visible region showed that PEB139α/PEB158β and PEB82α/PEB82β existed as two exciton-coupled bilin pairs. Energy transfer within the exciton-coupled pairs was by exciton splitting, while between the exciton-coupled pairs was by F?rster resonance. From the studies of the energy transfer dynamics by ultrafast time-resolved fluorescence spectroscopy, it was confirmed that the energy transfer of the B-PE hexamer had three time components of 8 ps, 60 ps, and 1200 ps. In addition, the internal energy transfer pathways of B-phycoerythrin hexamer were identified by deconvoluting the fluorescence decay curve at different detection wavelengths.
文章类型Article
资助机构National Natural Science Foundation of China; Government of Guangdong Province for NSF; Key Project of Coal-based Science and Technology in Shanxi Province; Scientific Research Foundation of Ludong University
收录类别SCI
语种英语
关键词[WOS]CRYSTAL-STRUCTURE; C-PHYCOCYANIN; PURIFICATION; CRUENTUM; PHYCOBILISOMES; PHYCOBILIPROTEINS; RESOLUTION; MODEL
研究领域[WOS]Physics, Multidisciplinary
WOS记录号WOS:000558937700004
引用统计被引频次：1[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/25266
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Xie, MY; Qin, S作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Laishan Dist 264003, Yantai, Peoples R China
2.Sun Yat Sen Univ, State Key Lab Optoelect Mat & Technol, Sch Phys, Guangzhou 510275, Guangdong, Peoples R China
3.? Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
4.Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China
5.Beijing Inst Technol, Sch Informat Technol, Zhuhai 519088, Zhuhai, Peoples R China
推荐引用方式
GB/T 7714Li WJ,Pu, Y,Tang, ZH,et al. Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum[J]. Chinese Journal of Physics,2020,66:24-35.
APALi WJ,Pu, Y,Tang, ZH,Zhao, FL,Xie, MY,&Qin, S.(2020).Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum.Chinese Journal of Physics,66,24-35.
MLALi WJ,et al."Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum".Chinese Journal of Physics 66(2020):24-35.
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