其他题名Isolation of Cellulase-producing Fungi and Screening of Highly Efficient Strains
邵帅; 王玲玲; 林剑; 徐世艾; 李岩
发表期刊河南农业科学
ISSN1004-3268
2020-02-27
卷号49期号:04页码:55-61
关键词纤维素酶真菌鉴定优化酶活性
产权排序(1) 烟台大学生命科学学院; (2) 中国科学院烟台海岸带研究所
作者部门海岸带生物学与生物资源保护实验室
英文摘要In order to isolate the highly efficient cellulose-degrading strain,a total of 24 fungi isolates were obtained from the decomposing fruit tree branches by using the dilution isolation method,with sodium carboxymethyl cellulose as the sole carbon substrate.Eight fungi strains were found to produce extracellular cellulase.Strains with larger hydrolytic circle/colony diameter in preliminary tests were selected to detect their celluase activities,and the results indicated strain 10 with highest activities.Its filter paper enzyme activity,endonuclease activity and exonuclease activity reached 13.26,36.67 and 12.06 IU respectively.Based on molecular and phylogenetic analyses,the strain was identified as Aspergillus terreus.Next the fermentation conditions through single factor optimizing were improved,and the optimal culture conditions for the three enzymes production were pH=7,the sodium carboxymethyl cellulose content=1.25%,and the sodium nitrate content=1.50%.After optimization,the filter paper enzyme activity,endonuclease activity and exonuclease activity reached 27.80,57.30,29.10 IU,which increased by 109.65%,56.26%,141.29% compared to pre-optimization,respectively.The highly efficient cellulase producing fungus strain 10 was provided for fruit branches fermentation and cellulase industrial development.
中文摘要为分离高效降解纤维素的菌株,以羧甲基纤维素钠为唯一底物,从腐熟的苹果树枝条中选择性分离到24株真菌,利用刚果红染色法初筛测定,发现有8株菌具有产胞外纤维素酶的能力。对初筛试验中透明圈/菌落直径较大的菌株进行纤维素酶活测定,最终获得1株高效产胞外纤维素酶的菌株10,其滤纸酶活性、内切酶活性、外切酶活性分别达到13.26、36.67、12.06 IU。基于分子生物学鉴定和系统发育分析,表明该菌株为土曲霉(Aspergillus terreus)。通过单因素优化发酵条件,得到该菌最适产酶培养条件为pH=7、羧甲基纤维素钠含量1.25%、硝酸钠含量1.50%,优化后其滤纸酶活性、内切酶活性、外切酶活性达到27.80、57.30、29.10 IU,分别比优化前提高了109.65%、56.26%、141.29%。菌株10能够高效产生胞外纤维素酶,为果树枝条的腐熟利用和纤维素酶的工业化开发提供了高效菌株。
文章类型期刊论文
资助机构国家自然科学基金项目(31600009); 中国科学院烟台海岸带研究所前沿部署项目(Y855021012); 吉林省与中科院科技合作高新技术产业化资金项目(2019SYHZ0036); 河北省重点研发计划项目(19273802D); 烟台市科技计划项目(2018ZHGY074)
收录类别其他
语种中文
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/25181
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.烟台大学生命科学学院;
2.中国科学院烟台海岸带研究所
推荐引用方式
GB/T 7714邵帅,王玲玲,林剑,等. 产纤维素酶真菌分离及高效菌株筛选[J]. 河南农业科学,2020,49(04):55-61.
APA邵帅,王玲玲,林剑,徐世艾,&李岩.(2020).产纤维素酶真菌分离及高效菌株筛选.河南农业科学,49(04),55-61.
MLA邵帅,et al."产纤维素酶真菌分离及高效菌株筛选".河南农业科学 49.04(2020):55-61.
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