Xu, RF; Wang, Y
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发表期刊ANALYST
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ISSN0003-2654
2019-04-21
卷号144期号:8页码:2556-2564
关键词IMAGING IN-VITROSUPEROXIDE ANIONXANTHINE-OXIDASERATIONAL DESIGNLIVER ISCHEMIAFREE-RADICALSMITOCHONDRIAOXYGENELECTROCATALYSISPEROXYNITRITE
研究领域Chemistry, Analytical
DOI10.1039/c9an00243j
产权排序[Xu, Runfeng; You, Huiyan] Dalian Univ, Sch Environm & Chem Engn, Dalian 116622, Peoples R China; [Xu, Runfeng; Wang, Yue; Zhang, Liangwei; Wang, Yunqing; Chen, Lingxin] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wang, Yue] Univ Chinese Acad Sci, Beijing 10004, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
通讯作者You, Huiyan(dlyhy@dicp.ac.cn); Zhang, Liangwei(liangweizhang@yic.ac.cn); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Hydrogen peroxide (H2O2), as a major component of reactive oxygen species (ROS), plays an important role in normal physiological processes. A H2O2 burst also occurs in the ischemia/reperfusion (I/R) process and causes a series of physiological and pathological injuries. Therefore, it is important to determine concentration fluctuations of H2O2. Here we develop a ratiometric fluorescent probe, Cy-ArB, which shows high selectivity and sensitivity toward H2O2. The fluorescence response of the probe is triggered by the reaction of borate esters with H2O2, and this process releases a near-infrared heptamethine cyanine fluorophore which has the ability of mitochondrial tracing. Hence, the probe can be used for real-time monitoring of H2O2 fluctuations in the mitochondrial respiration chain. Finally, we explore the fluctuations of H2O2 in cells and in vivo during the I/R process using the probe Cy-ArB. The results of our experiments prove that our probe is a potential candidate for clinical surgery pre-evaluation.
文章类型Article
资助机构National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 21778026, 81573393]; Program of the Youth Innovation Promotion Association, CAS [2017256, 2019217]; National Key Scientific Instrument and Equipment Development Projects [2013YQ17052506]; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662]
收录类别SCI
语种英语
关键词[WOS]IMAGING IN-VITRO; SUPEROXIDE ANION; XANTHINE-OXIDASE; RATIONAL DESIGN; LIVER ISCHEMIA; FREE-RADICALS; MITOCHONDRIA; OXYGEN; ELECTROCATALYSIS; PEROXYNITRITE
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000467383200008
引用统计被引频次:9[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24954
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Dalian Univ, Sch Environm & Chem Engn, Dalian 116622, Peoples R China;
2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
3.Univ Chinese Acad Sci, Beijing 10004, Peoples R China;
4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
推荐引用方式
GB/T 7714Xu, RF,Wang, Y,You, HY,et al. A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury[J]. ANALYST,2019,144(8):2556-2564.
APAXu, RF,Wang, Y,You, HY,Zhang, LW,Wang, YQ,&Chen, LX.(2019).A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury.ANALYST,144(8),2556-2564.
MLAXu, RF,et al."A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury".ANALYST 144.8(2019):2556-2564.
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