Yang, DL
; Han, YJ; Chen, LZ; Liu, YL; Cao, RW; Wang, Q; Zhao, JM发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2019-05
卷号88页码:328-334
关键词Ruditapes philippinarumScavenger receptorPattern recognition receptorImmune recognition
研究领域Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
DOI10.1016/j.fsi.2019.03.009
产权排序[Yang, Dinglong; Han, Yijing; Liu, Yongliang; Cao, Ruiwen; Wang, Qing; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Wang, Qing; Zhao, Jianmin] Chinese Acad Sci, Res & Dev Ctr Efficient Utilknt Coastal Bioresour, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; [Han, Yijing; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
通讯作者Zhao, Jianmin(jmzhao@yic.ac.cn)
作者部门海岸带生物资源高效利用研究与发展中心
英文摘要In the present study, a scavenger receptor class B type I (designed as RpSR-BI) was cloned and characterized from manila clam Ruditapes philippinarum. The full-length cDNA of RpSR-BI was of 2000 bp, containing an open reading frame (ORF) of 1515 bp. Multiple alignments and phylogenetic analysis strongly suggested that RpSR-BI was a member of the scavenger receptors family. The mRNA transcript of RpSR-BI was constitutively expressed in all tested tissues, and mainly expressed in hepatopancreas and hemocytes. Generally, Vihrio anguillanon or Micrococcus luteus challenge induced the expression of RpSR-BI transcripts in hemocytes of manila clams. Recombinant protein of RpSR-BI (rRpSR-BI) could bind lipopolysaccharides, peptidoglycan and glucan, but not chitin in vitro. Coinciding with the PAMPs binding assay, a broad agglutination spectrum was displayed by rRpSR-BI including Gram-positive bacteria and Gram-negative bacteria. Moreover, rRpSR-BI could enhance the phagocytosis and chemotaxis of hemocytes. These results showed that RpSR-BI functioned as a pattern recognition receptor (PRR) with distinct recognition spectrum, and also as an opsonin involved in the innate immune response of R. philippinarum.
文章类型Article
资助机构Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196]; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023]; Two-Hundred Talents Plan of Yantai [Y839081021]; Youth Innovation Promotion Association, CAS [2016196]
收录类别SCI
语种英语
关键词[WOS]C-TYPE LECTIN; PROTEIN; RECOGNITION; PEPTIDOGLYCAN; EXPRESSION; ADHESION; CLONING; ACTS
研究领域[WOS]Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS记录号WOS:000465060500039
引用统计被引频次：5[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24947
专题海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
支撑部门_中国科学院牟平海岸带环境综合试验站
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China;
2.Chinese Acad Sci, Res & Dev Ctr Efficient Utilknt Coastal Bioresour, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
推荐引用方式
GB/T 7714Yang, DL,Han, YJ,Chen, LZ,et al. Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:328-334.
APAYang, DL.,Han, YJ.,Chen, LZ.,Liu, YL.,Cao, RW.,...&Zhao, JM.(2019).Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions.FISH & SHELLFISH IMMUNOLOGY,88,328-334.
MLAYang, DL,et al."Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions".FISH & SHELLFISH IMMUNOLOGY 88(2019):328-334.
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