Zhang, HX; Wang, Q
; Liu, SL; Huo, D; Zhao, JM; Zhang, LB; Zhao, Y; Sun, LN; Yang, HS发表期刊FRONTIERS IN MICROBIOLOGY
ISSN1664-302X
2019-06-04
卷号10页码:1165
关键词Apostichopus japonicusintestine regenerationintestinal microbiotagenomicmetagenomic
研究领域Microbiology
DOI10.3389/fmicb.2019.01165
产权排序[Zhang, Hongxia; Liu, Shilin; Huo, Da; Zhang, Libin; Sun, Lina; Yang, Hongsheng] Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China; [Zhang, Hongxia; Liu, Shilin; Huo, Da; Zhang, Libin; Sun, Lina; Yang, Hongsheng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China; [Zhang, Hongxia; Wang, Qing; Liu, Shilin; Huo, Da; Zhao, Jianmin; Zhang, Libin; Sun, Lina; Yang, Hongsheng] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China; [Wang, Qing; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China; [Huo, Da; Zhang, Libin; Sun, Lina; Yang, Hongsheng] Univ Chinese Acad Sci, Beijing, Peoples R China; [Zhao, Ye] Yantai Univ, Ocean Sch, Yantai, Peoples R China
通讯作者Sun, Lina(sunlina@qdio.ac.cn); Yang, Hongsheng(hshyang@qdio.ac.cn)
作者部门海岸带生物资源高效利用研究与发展中心
英文摘要Host-intestine microbiota interactions have been widely studied in aquatic animals, but these interactions in the intestine regeneration process of the sea cucumber Apostichopus japonicus have been rarely investigated. To understand how intestine regeneration impacts the developing intestinal microbiome composition and function, we performed a case study to characterize the intestinal microbial composition and functional genes of A. japonicus during intestine regeneration stages. High-throughput 16S rRNA gene sequencing revealed significantly different intestine microbiota compositions in different regeneration stages. The phylogenetic diversity and composition of the intestinal microbiota changed significantly in the early regeneration stage and tended to recover in the end stage. During the regeneration process, the abundance of Bacteroidetes and Rhodobacterales increased significantly. A network analysis revealed that Rhodobacteraceae and Flavobacteriaceae may function as keystone taxa in the intestinal microbial community of A. japonicus during intestine regeneration. Metagenomic analyses of representative samples revealed that the microbiomes of regenerating intestines were enriched in genes facilitating cell proliferation, digestion and immunity. The increased abundance of Bacteroidetes elevated the enrichment of genes associated with carbohydrate utilization. Some functional features in the subsystem category changed in a pattern that was consistent with the changing pattern of microbiota composition during intestine regeneration. Our results revealed that seemingly regular alterations in the intestinal microbiome composition and function are associated with intestine regeneration stages. Intestinal microbiota can increase the abundance of beneficial bacterial members and upregulate related functional genes to adapt to intestine regeneration and reconstruct a stable community structure. This study provides a new insight into the mechanism of the host-microbiota interaction response to organ regeneration.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41776162]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2018M642712]; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science; Pilot National Laboratory for Marine Science and Technology (Qingdao) [LMEES-YTSP-2018-01-09]; Agricultural Seed Project of Shandong Province [2016LZGC032]; Taishan Scholars Program; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023]; Two-Hundred Talents Plan of Yantai [Y839081021]; Youth Innovation Promotion Association CAS [2016196, 2019209]
收录类别SCI
语种英语
关键词[WOS]BACTERIAL DIVERSITY; GUT; GROWTH; EXPRESSION; VIBRIO; GENES; FLAVOBACTERIACEAE; IDENTIFICATION; HOLOTHUROIDEA; ALIGNMENT
研究领域[WOS]Microbiology
WOS记录号WOS:000470133300001
引用统计被引频次：5[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24922
专题海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China;
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China;
5.Univ Chinese Acad Sci, Beijing, Peoples R China;
6.Yantai Univ, Ocean Sch, Yantai, Peoples R China
推荐引用方式
GB/T 7714Zhang, HX,Wang, Q,Liu, SL,et al. Genomic and Metagenomic Insights Into the Microbial Community in the Regenerating Intestine of the Sea Cucumber Apostichopus japonicus[J]. FRONTIERS IN MICROBIOLOGY,2019,10:1165.
APAZhang, HX.,Wang, Q.,Liu, SL.,Huo, D.,Zhao, JM.,...&Yang, HS.(2019).Genomic and Metagenomic Insights Into the Microbial Community in the Regenerating Intestine of the Sea Cucumber Apostichopus japonicus.FRONTIERS IN MICROBIOLOGY,10,1165.
MLAZhang, HX,et al."Genomic and Metagenomic Insights Into the Microbial Community in the Regenerating Intestine of the Sea Cucumber Apostichopus japonicus".FRONTIERS IN MICROBIOLOGY 10(2019):1165.
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