Wang, Y
; Zhang, LW; Zhang, SZ; Liu, Z; Chen, LX发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2019-06-18
卷号91期号:12页码:7812-7818
关键词MITOCHONDRIAL AUTOPHAGYOXIDATIVE STRESSHYDROGEN-SULFIDESYNTHASE CBSMERCURYPERSULFIDESCYSTEINESULFURDISULFIDEAPOPTOSIS
研究领域Chemistry, Analytical
DOI10.1021/acs.analchem.9b01511
产权排序[Wang, Yue; Zhang, Liangwei; Zhang, Songzi; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Re, Yantai 264003, Peoples R China; [Chen, Lingxin] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; [Liu, Zhe; Chen, Lingxin] Qufu Normal Univ, Dept Chem & Chem Engn, Qufu 273165, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China; [Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Glutathione hydropersulfides (GSSH) are alluded to play crucial roles in signal transduction, redox homeostasis, and metabolic regulation. However, the detailed biological functions of GSSH in these aspects are extremely ambiguous. The key barrier to understand the role of GSSH in biological systems is a lack of detection tools with high spatiotemporal resolution. To address the issues, we are seeking novel chemical tools for GSSH detection. We herein develop the first two-photon ratiometric fluorescent probe (TP-Dise) for GSSH detection with high spatial and temporal resolution in living cells and tissue. On the basis of our probe TP-Dise, we investigate the biosynthesis of GSSH, and the results indicate that GSSH is mainly from two sulfurtransferases, CBS and CSE. Furthermore, we explore the biological function of GSSH in protecting cells from mercury ion-induced cell damage for the first time. The experimental results indicate that mercury ions may induce cell death by causing mitochondrial autophagy. GSSH acts both as antagonist and as antioxidant and can effectively alleviate the damage caused by mercury stress.
文章类型Article
资助机构National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 21778026]; program of Youth Innovation Promotion Association, CAS [2019217]
收录类别SCI
语种英语
关键词[WOS]MITOCHONDRIAL AUTOPHAGY; OXIDATIVE STRESS; HYDROGEN-SULFIDE; SYNTHASE CBS; MERCURY; PERSULFIDES; CYSTEINE; SULFUR; DISULFIDE; APOPTOSIS
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000472682000042
引用统计被引频次：21[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24919
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Re, Yantai 264003, Peoples R China;
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China;
3.Qufu Normal Univ, Dept Chem & Chem Engn, Qufu 273165, Peoples R China;
4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China;
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Wang, Y,Zhang, LW,Zhang, SZ,et al. High Spatiotemporal Resolution Observation of Glutathione Hydropersulfides in Living Cells and Tissue via a Two-Photon Ratiometric Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2019,91(12):7812-7818.
APAWang, Y,Zhang, LW,Zhang, SZ,Liu, Z,&Chen, LX.(2019).High Spatiotemporal Resolution Observation of Glutathione Hydropersulfides in Living Cells and Tissue via a Two-Photon Ratiometric Fluorescent Probe.ANALYTICAL CHEMISTRY,91(12),7812-7818.
MLAWang, Y,et al."High Spatiotemporal Resolution Observation of Glutathione Hydropersulfides in Living Cells and Tissue via a Two-Photon Ratiometric Fluorescent Probe".ANALYTICAL CHEMISTRY 91.12(2019):7812-7818.
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