Min, J
; Xu, LX; Fang, SY; Chen, WW; Hu, XK发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
2019-09
卷号103期号:18页码:7741-7750
关键词124-Benzenetriol pathway2-Chloro-4-nitrophenolCatabolic mechanismGram-negative bacteriumhnp clusterTwo-component monooxygenase
研究领域Biotechnology & Applied Microbiology
DOI10.1007/s00253-019-09994-7
产权排序[Min, Jun; Xu, Lingxue; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; [Min, Jun; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China; [Xu, Lingxue] Yantai Univ, Coll Life Sci, Yantai, Peoples R China
通讯作者Hu, Xiaoke(xkhu@yic.ac.cn)
作者部门海岸带生物学与生物资源保护实验室
英文摘要2-Chloro-4-nitrophenol (2C4NP) is the most common chlorinated nitrophenol pollutant, and its environmental fate is of great concern. Cupriavidus sp. CNP-8, a Gram-negative bacterium, has been reported to degrade 2C4NP via the 1,2,4-benzenetriol (BT) pathway, significantly different from the (chloro)hydroquinone pathways reported in all other Gram-negative 2C4NP-utilizers. Herein, the BT pathway of the catabolism of 2C4NP in this strain was characterized at the molecular, biochemical, and genetic levels. The hnp gene cluster was suspected to be involved in the catabolism of 2C4NP because the hnp genes are significantly upregulated in the 2C4NP-induced strain CNP-8 compared to the uninduced strain. HnpAB, a two-component FAD-dependent monooxygenase, catalyzes the conversion of 2C4NP to BT via chloro-1,4-benzoquinone, with a K-m of 2.7 +/- 1.1 mu M and a k(cat)/K-m of 0.17 +/- 0.03 mu M-1 min(-1). hnpA is necessary for strain CNP-8 to utilize 2C4NP in vivo. HnpC, a BT 1,2-dioxygenase, was proved to catalyze BT ring-cleavage with formation of maleylacetate by HPLC-MS analysis. Phylogenetic analysis indicated that HnpA likely has different evolutionary origin compared to other functionally identified 2C4NP monooxygenases. To our knowledge, this is the first report revealing the catabolic mechanism of 2C4NP via the BT pathway in a Gram-negative bacterium, increasing our knowledge of the catabolic diversity for microbial 2C4NP degradation at the molecular and biochemical level.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31600085]; State's Key Project of Research and Development Plan [2016YFC1402300]; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013]; Yantai Science and Technology Project [2017ZH092]; State Key Laboratory of Microbial Metabolism, Shanghai JiaoTong University [MMLKF17-04]
收录类别SCI
语种英语
关键词[WOS]GENE-CLUSTER; PSEUDOMONAS-STUTZERI; P-NITROPHENOL; MONOOXYGENASE CATALYZES; CATABOLISM PATHWAY; STRAIN; BIODEGRADATION; 4-NITROPHENOL; 2,4,6-TRICHLOROPHENOL; IDENTIFICATION
研究领域[WOS]Biotechnology & Applied Microbiology
WOS记录号WOS:000483662400030
引用统计被引频次：3[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24871
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China;
3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
推荐引用方式
GB/T 7714Min, J,Xu, LX,Fang, SY,et al. Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103(18):7741-7750.
APAMin, J,Xu, LX,Fang, SY,Chen, WW,&Hu, XK.(2019).Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103(18),7741-7750.
MLAMin, J,et al."Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.18(2019):7741-7750.
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