Zhao, Y; Wang, Q
; Liu, H; Li, BJ; Zhang, HX发表期刊JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
2019-09
卷号37期号:5页码:1714-1725
关键词gut microbiotasea cucumberantibiotic16S rRNA geneillumina sequencing
研究领域Limnology; Oceanography
DOI10.1007/s00343-019-8308-5
产权排序[Zhao Ye; Li Bingjun] Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China; [Wang Qing; Liu Hui; Zhang Hongxia] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
通讯作者Zhao Ye(zyzy19872006@ytu.edu.cn); Liu Hui(huiliu@yic.ac.cn)
作者部门海岸带生物资源高效利用研究与发展中心
英文摘要Sea cucumber Apostichopus japonicus is an important marine economic species in Asian countries due to its profound nutritional and medicinal value. So far, with the rapid development of intensified artificial aquaculture of sea cucumbers, the use of antibiotics is still an inexpensive and dispensable way to treat pathogenic infections, especially during the nursery phase. However, there is little information on the effects of antibiotics on the intestinal microbiota of sea cucumber. Therefore an Illumina based sequencing method was used to examine the intestinal bacterial composition of juvenile A. japonicas following diets with three typical antibiotics (tetracycline, erythromycin, and norfloxacin) under 15, 30, and 45 d. The findings reveal that different antibiotics have distinct effects on the growth performance of juvenile sea cucumbers. However, the richness and diversity of microbiota were barely affected by antibiotics but the community composition alterations indicated that the three antibiotics exhibited their respective patterns of reshaping the intestinal bacteria of juvenile sea cucumbers. In common, the abundance of some sensitive genera with helpful functions, such as Thalassotalea, Shewanella, Sulfitobacter, and Halomonas decreased significantly with exposure to antibiotics and the abundance of multiple potential pathogenic- and suspected antibiotic-resistant microorganisms like Arcobacter, Leucothrix, and Clostridium_sensu_stricto_1 was found increased significantly in the antibiotic groups. These results suggest that low doses of antibiotics could affect the composition of the intestinal microbiota of sea cucumbers and might increase the risk of infection of the hosts. This study could help us to explore how antibacterial compounds modify the gut microbiota of sea cucumbers and provide theoretical guidance in hatchery management by scientific antibiotic use in sea cucumber mariculture.
文章类型Article
资助机构Shandong Provincial Natural Science FoundationNatural Science Foundation of Shandong Province [ZR2017BD026]; Yantai University Doctoral Start-up Foundation [HX15B14]; Demonstration Project on Innovative Development of Marine Economy Foundation [YHCX-SW-P-201701]
收录类别SCI
语种英语
关键词[WOS]INTESTINAL MICROBIOTA; BACTERIAL COMMUNITIES; DIVERSITY; DISEASE; HOST; RESISTANCE; SHRIMP; OXYTETRACYCLINE; MICROFLORA; PATHOGENS
研究领域[WOS]Limnology; Oceanography
WOS记录号WOS:000484533400023
引用统计被引频次：3[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24870
专题海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位1.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Zhao, Y,Wang, Q,Liu, H,et al. High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(5):1714-1725.
APAZhao, Y,Wang, Q,Liu, H,Li, BJ,&Zhang, HX.(2019).High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(5),1714-1725.
MLAZhao, Y,et al."High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.5(2019):1714-1725.
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