Lv, JS; Zhao, JM
; Yang, DL; Wu, HF; Cong, M发表期刊JOURNAL OF INVERTEBRATE PATHOLOGY
ISSN0022-2011
2019-09
卷号166页码:107215
关键词Mytilus galloprovincialisMytimacin-4Tissue distributionRecombinant proteinsAntimicrobial activity
研究领域Zoology
DOI10.1016/j.jip.2019.107215
产权排序[Lv, Jiasen] Yantai Univ, Biol Sch, Yantai 264005, Peoples R China; [Zhao, Jianmin; Yang, Dinglong; Wu, Huifeng; Cong, Ming] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China
通讯作者Cong, Ming(mcong@yic.ac.cn)
作者部门海岸带环境过程实验室
英文摘要Antimicrobial peptides (AMPs) play fundamental roles in the innate immunity of invertebrates. Mytimacin-4 is a kind of AMP gene previously sequenced from Mytilus galloprovincialis based on an identified EST sequence in our lab. In the present study, the tissue distribution and antimicrobial activities of mytimacin-4 were further investigated. A qRT-PCR analysis revealed that mytimacin-4 transcripts were constitutively expressed in all of the tested tissues of M. galloprovincialis, with the highest expression level in the posterior adductor muscle. After challenge by Vibrio anguillarum, the expression level of mytimacin-4 gene was significantly increased at 24 h (P < 0.05) in the mantle and increased at 48 h (P < 0.05) in the posterior adductor muscle. This finding suggested that mytimacin-4 transcripts were inducible upon pathogen infection. A minimal inhibitory concentration (MIC) assay indicated that recombinant mytimacin-4 protein had potent antimicrobial activities against gram-positive and gram-negative bacteria. Among the tested microorganisms, mytimacin-4 protein exhibited strong inhibition activities against Bacillus subtilis and Vibrio parahaemolyticus with MICs of 0.315 mu M and 0.62 mu M respectively. This study provides for the first time direct evidence of antimicrobial action of mytimacin-4 in M. galloprovincialis.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [NSFC 41876135]
收录类别SCI
语种英语
关键词[WOS]RICH ANTIMICROBIAL PEPTIDE; MOLECULAR CHARACTERIZATION; ANTIBACTERIAL PEPTIDES; HEMOCYTES; INVERTEBRATES; CHALLENGE; SEAWATER; IMMUNITY; BINDING; LEECH
研究领域[WOS]Zoology
WOS记录号WOS:000485335900010
引用统计被引频次：1[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24867
专题海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China;
2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China
推荐引用方式
GB/T 7714Lv, JS,Zhao, JM,Yang, DL,et al. Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2019,166:107215.
APALv, JS,Zhao, JM,Yang, DL,Wu, HF,&Cong, M.(2019).Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis.JOURNAL OF INVERTEBRATE PATHOLOGY,166,107215.
MLALv, JS,et al."Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis".JOURNAL OF INVERTEBRATE PATHOLOGY 166(2019):107215.
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