Ma, CB; Li, WJ
; Ge, BS; Lin, J; Qin, S发表期刊JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
2020-03
卷号38期号:2页码:529-538
关键词combinational biosynthesisphycocyanobilinresponse surface methodologyantioxidant
研究领域Limnology; Oceanography
DOI10.1007/s00343-019-9060-6
产权排序[Ma, Chengbo; Lin, Jian] Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China; [Li, Wenjun; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Ge, Baosheng] China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; [Li, Wenjun; Qin, Song] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
通讯作者Lin, Jian(linjian3384@163.com); Qin, Song(sqin@yic.ac.cn)
作者部门海岸带生物学与生物资源保护实验室
英文摘要The recombinant expression of phycocyanobilin (PCB) was carried out in Escherichia coli, and the best fermentation conditions of recombinant E. coli biosynthesized PCB are optimized in the response surface methodology to improve PCB production. The recombinant PCB is extracted, isolated, and purified by methanol and chloroform extraction. Recombinant PCB is validated in UV-vis spectroscopy, high-pressure liquid chromatography, and mass spectrometry. In addition, the anti-oxidant activities of the recombinant PCB are determined. The best induction conditions that optimized by Design Expert 8.0 software include: lactose concentration 4 mmol/L, induction temperature 24.69 degrees C, induction time 4.6 h, and induction duration 13.57 h, under which the PCB expression level reached approximately 13 mg PCB/L, which is more than four times of previously reported 3 mg PCB/L. The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm. The recombinant PCB has a good ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals.
文章类型Article
资助机构National Natural Science Foundation of China; Key Research and Development Program of Yantai
收录类别SCI
语种英语
关键词[WOS]SP PCC 6803; C-PHYCOCYANIN; ANTIOXIDANT; CHROMOPHORES; BILIPROTEINS; EXPRESSION
研究领域[WOS]Limnology; Oceanography
WOS记录号WOS:000522834800019
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24855
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
3.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China;
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714Ma, CB,Li, WJ,Ge, BS,et al. Biosynthesis of phycocyanobilin in recombinant Escherichia coli[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(2):529-538.
APAMa, CB,Li, WJ,Ge, BS,Lin, J,&Qin, S.(2020).Biosynthesis of phycocyanobilin in recombinant Escherichia coli.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(2),529-538.
MLAMa, CB,et al."Biosynthesis of phycocyanobilin in recombinant Escherichia coli".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.2(2020):529-538.
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