Xu, HD1,2; Xiao, LL2
; Zheng, SL2; Zhang, YC2,3,4; Li, JJ2,3,4; Liu, FH2,3发表期刊SCIENCE CHINA-TECHNOLOGICAL SCIENCES
ISSN1674-7321
2019-10
卷号62期号:10页码:1688-1694
关键词chloramphenicolGeobacter metallireducensdechlorinationextracellular electron transferreduction
研究领域Engineering, Multidisciplinary; Materials Science, Multidisciplinary
DOI10.1007/s11431-018-9415-2
产权排序[Xu HengDuo] Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
; [Xu HengDuo; Xiao LeiLei; Zheng ShiLing; Zhang YueChao; Li JiaJia; Liu FangHua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China; [Zhang YueChao; Li JiaJia; Liu FangHua] Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; [Zhang YueChao; Li JiaJia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者Liu FangHua(fhliu@yic.ac.cn)
作者部门海岸带生物学与生物资源保护实验室
英文摘要Geobacter metallireducens is known to be capable of removing nitroaromatic compounds via an oxidation mode. However, little attention has been paid to investigate the reductive removal of chlorinated nitroaromatic compounds by G. metallireducens. In this study, G. metallireducens was used to reduce chloramphenicol (CAP), a typical chlorinated nitroaromatic antibiotic. Cyclic voltammograms and chronoamperometry highlighted a higher peak current for CAP reduction by G. metallireducens compared to the control without bacteria. G. metallireducens efficiently reduced CAP (20 mg/L) with acetate as the sole electron donor, and the removal efficiency reached (97.6 +/- 4.9)% within 6 d. Aromatic amine (AMCl2), AMCl (dechlorinated AMCl2) and AM (dechlorinated AMCl) were identified as reduction products by liquid chromatography-mass spectrometry. However, the removal efficiency declined to (25.0 +/- 3.6)% when the CAP dosage increased to 80 mg/L. Transcriptomic analysis indicated the significant upregulation of genes related to electron transfer, such as pilus assembly protein gene (2.8 folds), NADH-quinone oxidoreductase subunit K2 gene (4.5 folds) and many c-type cytochrome genes such as cytochrome c biogenesis protein ResB (Gmet 2901, 4.6 folds), cytochrome c (Gmet 0335, 4.4 folds) and cytochrome c7 (Gmet 2902, 3.4 folds). Furthermore, a gene related to chlorinated contaminant removal (Gmet 1046, 5.4 folds) was also upregulated, possibly resulting in enhanced CAP reduction. This work deepened our knowledge of the bioremediation ability of G. metallireducens with respect to environmental contaminants and provided a potential strategy to treat antibiotics with electrochemically active bacteria.
文章类型Article
资助机构Postdoctoral Science Foundation of ChinaChina Postdoctoral Science Foundation [2018M632735]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [91751112, 41573071]; Senior User Project of RV KEXUE [KEXUE2018G01]; Natural Science FoundationNational Natural Science Foundation of China [JQ201608]; Young Taishan Scholars Program of Shandong Province [tsqn20161054]
收录类别SCI
语种英语
关键词[WOS]CARBON; PHARMACEUTICALS; FLORFENICOL; RESISTANCE; MECHANISM; OXIDATION; ELECTRODE; REMOVAL; BIOFILM; METAL
研究领域[WOS]Engineering, Multidisciplinary; Materials Science, Multidisciplinary
WOS记录号WOS:000504097800004
引用统计被引频次：8[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24838
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Liu, FH作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China;
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Xu, HD,Xiao, LL,Zheng, SL,et al. Reductive degradation of chloramphenicol by Geobacter metallireducens[J]. SCIENCE CHINA-TECHNOLOGICAL SCIENCES,2019,62(10):1688-1694.
APAXu, HD,Xiao, LL,Zheng, SL,Zhang, YC,Li, JJ,&Liu, FH.(2019).Reductive degradation of chloramphenicol by Geobacter metallireducens.SCIENCE CHINA-TECHNOLOGICAL SCIENCES,62(10),1688-1694.
MLAXu, HD,et al."Reductive degradation of chloramphenicol by Geobacter metallireducens".SCIENCE CHINA-TECHNOLOGICAL SCIENCES 62.10(2019):1688-1694.
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