Liu, XL; Xie, ZH
发表期刊MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN0894-0282
2019-11
卷号32期号:11页码:1547-1556
关键词genetics and gene regulationrhizobium-legume symbiosis
研究领域Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences
DOI10.1094/MPMI-05-19-0143-R
产权排序[Liu, Xiaolin; Xie, Zhihong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; [Liu, Xiaolin] Univ Chinese Acad Sci, Beijing, Peoples R China; [Liu, Xiaolin; Xie, Zhihong] Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Shandong, Peoples R China
通讯作者Xie, Zhihong(zhxie@yic.ac.cn)
作者部门海岸带生物学与生物资源保护实验室
英文摘要Azorhizobium caulinodans can form root and stem nodules with the host plant Sesbania rostrata. The role of the CheZ phosphatase in the A. caulinodans chemotaxis pathway was previously explored using the nonchemotactic cheZ mutant strain (AC601). This mutant displayed stronger attachment to the root surface, enhancing early colonization; however, this did not result in increased nodulation efficiency. In this study, we further investigated the role of CheZ in the interaction between strain ORS571 and the roots of its host plant. By tracking long-term colonization dynamic of cheZ mutant marked with LacZ, we found a decrease of colonization of the cheZ mutant during this process. Furthermore, the cheZ mutant could not spread on the root surface freely and was gradually outcompeted by the wild type in original colonization sites. Quantitative reverse-transcription PCR analyses showed that exp genes encoding exopolysaccharides synthesis, including oac3, were highly expressed in the cheZ mutant. Construction of a strain carrying a deletion of both cheZ and oac3 resulted in a mutant strain defective in the colonization process to the same extent as found with the oac3 single-mutant strain. This result suggested that the enhanced colonization of the cheZ mutant may be achieved through regulating the formation of exopolysaccharides. This shows the importance of the chemotactic proteins in the interaction between rhizobia and host plants, and expands our understanding of the symbiosis interaction between rhizobium and host plant.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31570063, 31870020]; National Natural Science Foundation of China (NSFC-Shandong Joint Fund Key Project) [U1806206]; Key Deployment Project of the Chinese Academy of Sciences [KFZD-SW-112]; Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences [+CXGC2016B10]
收录类别SCI
语种英语
关键词[WOS]SIGNAL-TRANSDUCTION CASCADE; NODULE DEVELOPMENT; ROOT COLONIZATION; BIOFILM FORMATION; CHEMOTAXIS; LIPOPOLYSACCHARIDE; NODULATION; SYSTEM; EXOPOLYSACCHARIDE; POLYSACCHARIDE
研究领域[WOS]Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences
WOS记录号WOS:000494258900011
引用统计被引频次：3[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24825
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China;
2.Univ Chinese Acad Sci, Beijing, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Shandong, Peoples R China
推荐引用方式
GB/T 7714Liu, XL,Xie, ZH. Inactivation of the Phosphatase CheZ Alters Cell-Surface Properties of Azorhizobium caulinodans ORS571 and Symbiotic Association with Sesbania rostrata[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2019,32(11):1547-1556.
APALiu, XL,&Xie, ZH.(2019).Inactivation of the Phosphatase CheZ Alters Cell-Surface Properties of Azorhizobium caulinodans ORS571 and Symbiotic Association with Sesbania rostrata.MOLECULAR PLANT-MICROBE INTERACTIONS,32(11),1547-1556.
MLALiu, XL,et al."Inactivation of the Phosphatase CheZ Alters Cell-Surface Properties of Azorhizobium caulinodans ORS571 and Symbiotic Association with Sesbania rostrata".MOLECULAR PLANT-MICROBE INTERACTIONS 32.11(2019):1547-1556.
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