Liu, YM; Cui, YL
; Chen, J; Qin, S; Chen, G发表期刊ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
2019-12
卷号44页码:UNSP 101679
关键词Synechocystis sp. PCC6803Astaxanthinbeta-Carotene ketolase (bkt)Carotenoid hydroxylase (crtR-B)Metabolic engineering
研究领域Biotechnology & Applied Microbiology
DOI10.1016/j.algal.2019.101679
产权排序[Liu, Yaming; Cui, Yulin; Chen, Jun; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China; [Liu, Yaming] Univ Chinese Acad Sci, Beijing 101418, Peoples R China; [Chen, Gao] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China; [Chen, Gao] Shandong Prov Key Lab Acad Crop Genet Improvement, Jinan 250100, Shandong, Peoples R China
通讯作者Qin, Song(sqin@yic.ac.cn); Chen, Gao(gxchen001@hotmail.com)
作者部门海岸带生物学与生物资源保护实验室
英文摘要Astaxanthin is a carotenoid with wide applications because of its strong antioxidant properties. It is mainly produced from the green alga Haematococcus pluvialis, but large-scale production remains a challenge. To provide another source, here, Synechocystis sp. PCC6803 was adopted as the chassis cell, and its carotenoid biosynthesis pathway was genetically extended to produce astaxanthin. The beta-carotene ketolase gene (bkt) and carotenoid hydroxylase gene (crtR-B) from H. pluvialis, which wild-type Synechocystis sp. PCC6803 lacks, were optimized based on the codon preference of Synechocystis sp. PCC6803 and heterologously expressed in that bacterium to create a strain capable of producing astaxanthin. The Synechocystis sp. PCC6803 mutant synthesized 4.81 +/- 0.06 mg astaxanthin per gram of cells (dry weight) after nitrogen starvation for 14 days. More than 30% of the total astaxanthin was in the free form, and 82.61% was the strong antioxidant 3S, 3'S-astaxanthin isoform. These results show the industrial application potential of metabolically-engineered Synechocystis sp. PCC6803 for astaxanthin production.
文章类型Article
资助机构National Key PD Program [2016YFE0106700]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41876188]; Natural Science Foundation of Shandong Province, ChinaNatural Science Foundation of Shandong Province [ZR2018ZB0210, ZR2016CM48]; Key Research and Development Program of Shandong [2018GSF121019, 2019GSF107098]; Project of Innovation & Development of Marine Economy [HHCL201803]; seed project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences [YIC755031013]; Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-STS-ZDTP-023]
收录类别SCI
语种英语
关键词[WOS]HAEMATOCOCCUS-PLUVIALIS; CRTR-B; MICROALGAE; ACCUMULATION; CYANOBACTERIA; CAROTENOIDS; ISOMERS
研究领域[WOS]Biotechnology & Applied Microbiology
WOS记录号WOS:000498792900039
引用统计被引频次：7[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24805
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 101418, Peoples R China;
3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China;
4.Shandong Prov Key Lab Acad Crop Genet Improvement, Jinan 250100, Shandong, Peoples R China
推荐引用方式
GB/T 7714Liu, YM,Cui, YL,Chen, J,et al. Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,44:UNSP 101679.
APALiu, YM,Cui, YL,Chen, J,Qin, S,&Chen, G.(2019).Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,44,UNSP 101679.
MLALiu, YM,et al."Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 44(2019):UNSP 101679.
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