Lin, B; Cui, YL
; Yan, MY; Wang, YC; Gao, ZQ; Meng, CX; Qin, S发表期刊ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
2019-12
卷号44页码:UNSP 101697
关键词Astaxanthinbeta-Carotene hydroxylasebeta-Carotenoid ketolaseChloroplast transformationDunaliella viridisHaematococcus pluvialis
研究领域Biotechnology & Applied Microbiology
DOI10.1016/j.algal.2019.101697
产权排序[Lin, Bin; Gao, Zhengquan; Meng, Chunxiao] Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China; [Cui, Yulin; Wang, Yinchu; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China; [Yan, Mingyan] Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Shandong, Peoples R China
通讯作者Meng, Chunxiao(mengchunxiao@126.com); Qin, Song(sqin@yic.ac.cn)
作者部门海岸带生物学与生物资源保护实验室
英文摘要Dunaliella viridis is a green microalga containing beta-carotene, which is the precursor of astaxanthin, the most active antioxidant in Haematococcus pluvialis. Two key enzymes of H. pluvialis, beta-carotene hydroxylase (CRTR-B) and beta-carotenoid ketolase (BKT), are required for converting beta-carotene to astaxanthin in D. viridis via the astaxanthin biosynthetic pathway. Considering the location of beta-carotene in the chloroplast of D. viridis, the two modified genes encoding BKT and CRTR-B in H. pluvialis were integrated via homologous recombination into the chloroplast genome, in this study. In the chloroplast, the homologous recombination vector pMD-bkt-crtr (16S-TrnA-atpA-bkt-crtR-B-rbcL-psbA-bar-TrnI-23S), bkt and crtR-B were regulated by the atpA promoter in a polycistron. The presence of astaxanthin in the D. viridis mutant expressing BKT and CRTR-B was verified using high performance liquid chromatography (HPLC), and the maximum content of total astaxanthin and canthaxanthin after high light induction were 77.5 +/- 7.7 and 50.1 +/- 0.8 mu g g(-1) in dry weight, respectively. Our results indicate that D. viridis can be used as a cell factory for astaxanthin production.
文章类型Article
资助机构National Key PD Program [2016YFE0106700]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41876188]; Shandong Provincial Natural Science Foundation, ChinaNatural Science Foundation of Shandong Province [ZR2018ZB0210]; Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-STS-ZDTP-023]; Project of Innovation & Development of Marine Economy [HHCL201803]; seed project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences [YIC755031013]; Key Research and Development Program of Shandong Province (Food for Special Medical Purpose) [2018YYSP016]; Key Research and Development Program of Shandong Province [2016GSF121030, 2017GSF21105]
收录类别SCI
语种英语
关键词[WOS]HAEMATOCOCCUS-PLUVIALIS; EXPRESSION; GENES; EXTRACTION; BIOSYNTHESIS; ACCUMULATION; PURIFICATION; SYSTEM; SALINA
研究领域[WOS]Biotechnology & Applied Microbiology
WOS记录号WOS:000498792900017
引用统计被引频次：8[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24804
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China;
3.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Shandong, Peoples R China
推荐引用方式
GB/T 7714Lin, B,Cui, YL,Yan, MY,et al. Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,44:UNSP 101697.
APALin, B.,Cui, YL.,Yan, MY.,Wang, YC.,Gao, ZQ.,...&Qin, S.(2019).Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,44,UNSP 101697.
MLALin, B,et al."Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 44(2019):UNSP 101697.
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