Zhang, Xiaoyu; He, Na; Huang, Yan; Yu, Fabiao; Li, Bowei
; Lv, Changjun; Chen, Lingxin发表期刊SENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
2019-03-01
卷号282页码:69-77
关键词Fluorescent probesNear-infrared fluorescenceCysteineMitochondria targetLung cancer
研究领域Chemistry; Electrochemistry; Instruments & Instrumentation
DOI10.1016/j.snb.2018.11.056
产权排序[Zhang, Xiaoyu; He, Na; Lv, Changjun] Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China; [Zhang, Xiaoyu; He, Na; Huang, Yan; Li, Bowei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Yu, Fabiao] Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China; [Zhang, Xiaoyu; He, Na; Yu, Fabiao; Lv, Changjun] Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要Cysteine (Cys) plays significant roles in many physiological processes, although its normal concentration is maintained at the micromole level. Abnormally high levels of intracellular Cys can lead to many diseases including cancer. Recent years, many effective fluorescent probes have been developed for the selective detection of Cys against other biological thiols. Herein, we synthesized a ratiometric near-infrared (NIR) fluorescent probe Cy-OAcr for selective imaging of intracellular Cys. Cy-OAcr has a lipophilic iminium cation unit as the mitochondrial guider and an acrylate group as the Cys recognition unit as well as a fluorescence modulator for rearranging the conjugated Tc-electron system of cyanine fluorophore. Upon detection of Cys, there occurs a significant absorption and fluorescence spectral shift, which are desirably beneficial for ratiometric detection. This probe has high sensitivity and selectivity for Cys detection over glutathione (GSH), homocysteine (Hcy), and other biomolecules with a low limit of detection at 0.09 mu M. Probe Cy-OAcr is capable to detect and image Cys in three living cancer cell lines and their corresponding tumor-bearing mice models. More importantly, we successfully apply this fluorescent probe to evaluate the level of Cys in orthotopic lung cancer model. Imaging analyses reveal that the probe can discriminate tumor lesions from normal tissues, indicating its significant potential applications for clinical auxiliary diagnosis of cancer.
文章类型Article
资助机构National Nature Science Foundation of China [21775162, 21864011, 31470415, 81670064, 21575159, 41776110]; Talent Program of Hainan Medical University [XRC180006, XRC180007]; Hundred-Talent Program (Hainan); Medical Science and Technology Development Project of Shandong Province [2017WS800]; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22]
收录类别SCI
语种英语
关键词[WOS]TURN-ON PROBE; HYDROGEN-SULFIDE; IN-VIVO; ENDOGENOUS CYSTEINE; OXIDATIVE STRESS; LIVING CELLS; GLUTATHIONE; HOMOCYSTEINE; EMISSION; THIOLS
研究领域[WOS]Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation
WOS记录号WOS:000455090000009
引用统计被引频次：45[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24640
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China;
4.Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China;
5.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
推荐引用方式
GB/T 7714Zhang, Xiaoyu,He, Na,Huang, Yan,et al. Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,282:69-77.
APAZhang, Xiaoyu.,He, Na.,Huang, Yan.,Yu, Fabiao.,Li, Bowei.,...&Chen, Lingxin.(2019).Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice.SENSORS AND ACTUATORS B-CHEMICAL,282,69-77.
MLAZhang, Xiaoyu,et al."Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice".SENSORS AND ACTUATORS B-CHEMICAL 282(2019):69-77.
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