Liu Jing-hua; Chen Jun; Qin Song
; Qi Ze-ming; Huang Qing发表期刊SPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
2019-01
卷号39期号:1页码:79-86
关键词Infrared spectroscopyMid-infrared spectroscopyNear-infrared reflectance spectroscopyMicrospectroscopy imagingSynchrotron radiationMicroalgae
研究领域Spectroscopy
DOI10.3964/j.issn.1000-0593(2019)01-0079-08
产权排序[Liu Jing-hua; Huang Qing] Anhui Sci & Technol Univ, Coll Life Sci, Fengyang 233100, Peoples R China; [Liu Jing-hua; Huang Qing] Chinese Acad Sci, Inst Tech Biol & Agr Engn, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China; [Chen Jun; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Qi Ze-ming; Huang Qing] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Microalgae are rich in carotenoids, vitamins, proteins, polyunsaturated fatty acids and other nutrients required by humans and animals. In addition, some microalgae also play critical roles in aquatic ecological environment. Thus, it is important to carry out microalgal research for practical applications. Traditionally, analysis of microalgal components requires cell disruption and extraction with organic solvents followed by gas or liquid chromatography of the extracts. However, these traditional methods are generally time-consuming, requiring expensive instruments and sophisticated operations. So it is urgent to develop a more effective method for rapid and convenient microalgal analysis. Infrared spectroscopy mainly relies on the absorbance of radiation at molecular vibrational frequencies to observe the changes of the molecular compositions, characteristics, structures and concentrations. Actually, this technique has emerged as a promising tool to distinguish and quantify various cellular components such as proteins, lipids, nucleic acids, carbohydrates, chlorophyll and carotenoids in microalgal systems. Compared with the conventional detection methods, it has the advantages of simple, fast, non-invasive, and multiplex measurements. Especially, with the development of microscopic imaging technology, infrared microspectroscopy shows the powerful potential for spatially resolved and real-time in situ measurements of biological systems at the single cell or tissue level. In recent years, with the development of synchrotron radiation technique, the advanced synchrotron infrared microspectroscopy and imaging technique has been applied and it can provide higher sensitivity and spatial resolution than the traditional infrared spectroscopy, so to a certain degree it resolves the contradiction between high throughput analysis and high spatial resolution observation. In this study, the principle and advantages of infrared spectroscopy and micro-spectroscopic imaging were firstly illustrated, especially the application of this technique combined with chemometrics in the field of biology. Then, the recent progress on the application of infrared spectroscopy in discrimination, metabolism, breeding, water environment protection, medicine and health relating to microalgal research was introduced and discussed in review of a variety of recent literature. For instance, infrared spectroscopy in combination with chemometrics can identify, discriminate or classify different microalgal strains. This technique can also be applied in the research of microalgal growth and metabolism based on its advantages of fast and multicomponent analysis. It can even provide a non-destructive and high-throughput method to screen the lipids, beta-carotene or astaxanthin hyperproducing microalgal strains based on infrared spectroscopic tool. In addition, microalgae have been reported to be potentially proper biosorbents for the treatment of heavy metals and dyes wastewater. Actually, infrared spectroscopy has already been applied as a proper approach to study biosorption of heavy metals or dyes from wastewaters using microalgae. Being a powerful tool, it has not only distinguished and quantified various vital components quickly and conveniently, but has also provided an effective method for quality detection and authenticity measurement of microalgal foods and drugs. Although there are still some deficiencies for the application of infrared spectroscopy in microalgal research, the promising potential and comprehensive resolution for it are discussed in this study. Finally, based on the latest progress and application of infrared spectroscopy, some prospects of this technique for microalgal research were also put forward at the end of the review, such as industrial large-scale culture of microalgae, selection and breeding of hyperproducing strains, the physiology of microalgae, the molecular structure and function of organelles, etc.
文章类型Article
收录类别SCI
语种英语
关键词[WOS]IR SPECTROSCOPY; MICROSPECTROSCOPY; FTIR; SPIRULINA; PERFORMANCE; MICROSCOPY; MUTANTS; STRESS; TOOL; QUANTIFICATION
研究领域[WOS]Spectroscopy
WOS记录号WOS:000456357000013
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24611
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Anhui Sci & Technol Univ, Coll Life Sci, Fengyang 233100, Peoples R China;
2.Chinese Acad Sci, Inst Tech Biol & Agr Engn, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China;
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
4.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China
推荐引用方式
GB/T 7714Liu Jing-hua,Chen Jun,Qin Song,et al. Application of Infrared Spectroscopy in Microalgal Research[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2019,39(1):79-86.
APALiu Jing-hua,Chen Jun,Qin Song,Qi Ze-ming,&Huang Qing.(2019).Application of Infrared Spectroscopy in Microalgal Research.SPECTROSCOPY AND SPECTRAL ANALYSIS,39(1),79-86.
MLALiu Jing-hua,et al."Application of Infrared Spectroscopy in Microalgal Research".SPECTROSCOPY AND SPECTRAL ANALYSIS 39.1(2019):79-86.
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