Lv, Enguang; Ding, Jiawang
; Qin, Wei发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2018-11-20
卷号90期号:22页码:13600-13606
关键词RAPID DETECTIONMAGNETIC BEADSRECOGNITIONMEMBRANEBACTERIOCINSBIOSENSORSYSTEMTRIFLUOROETHANOLMECHANISMSELECTRODES
研究领域Chemistry
DOI10.1021/acs.analchem.8b03809
产权排序[Lv, Enguang; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; [Lv, Enguang; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang; Qin, Wei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China; [Lv, Enguang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门海岸带环境过程实验室
英文摘要Peptide-based sandwich assays are promising tools in molecular detection, but may be restricted by the availability of "pairs" of affinity peptides. Herein, a new potentiometric sandwich assay for bacteria based on peptide pairs derived from an antimicrobial peptide (AMP) ligand is demonstrated. As a model, the original AMP with a welldefined structure for Listeria monocytogenes (LM) can be split into two fragments to serve as the peptide pairs for the sandwich assay. The recognition and binding of the short peptide pairs to the target can be verified by circular dichroism, flow cytometry, fluorometry, and optical microscopy. The potentiometric magnetic bead-based sandwich assay is designed by using horseradish peroxidase as a label. The enzyme can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine with H2O2 to induce a potential change on a polymeric membrane ion selective electrode. Under optimal conditions, the concentration of LM can be determined potentiometrically in a linear range of 1.0 X 10(2) to 1.0 x 10(6) CFU mL(-1) with a detection limit of 10 CFU mL(-1) (3 sigma). The proposed sensing strategy expands the applications of peptides in the field of bioassays.
文章类型Article
资助机构National Natural Science Foundation of China [21575158, 21475148, 41876108]; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020702]; Taishan Scholar Program of Shandong Province [TS20081159]; National Key Research and Development Program of China [2016YFC1400700]; Key Research and Development Program of Yantai [2018ZHGY053]
收录类别SCI
语种英语
关键词[WOS]RAPID DETECTION; MAGNETIC BEADS; RECOGNITION; MEMBRANE; BACTERIOCINS; BIOSENSOR; SYSTEM; TRIFLUOROETHANOL; MECHANISMS; ELECTRODES
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000451246100062
引用统计被引频次：20[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24579
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China;
2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China;
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Lv, Enguang,Ding, Jiawang,Qin, Wei. Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay[J]. ANALYTICAL CHEMISTRY,2018,90(22):13600-13606.
APALv, Enguang,Ding, Jiawang,&Qin, Wei.(2018).Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay.ANALYTICAL CHEMISTRY,90(22),13600-13606.
MLALv, Enguang,et al."Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay".ANALYTICAL CHEMISTRY 90.22(2018):13600-13606.
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