Gao, Min; Wang, Rui; Yu, Fabiao; Li, Bowei
; Chen, Lingxin发表期刊JOURNAL OF MATERIALS CHEMISTRY B
ISSN2050-750X
2018-11-07
卷号6期号:41页码:6637-6645
关键词HYDROGEN POLYSULFIDESLIVING CELLSZEBRAFISH EMBRYOSCYSTEINESULFIDEH2SPERSULFIDECONSTRUCTIONMODULATIONCHEMISTRY
研究领域Materials Science
DOI10.1039/c8tb01794h
产权排序[Gao, Min; Wang, Rui; Yu, Fabiao; Li, Bowei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Wang, Rui; Yu, Fabiao] Hainan Med Univ, Inst Funct Mat & Mol Imaging, Coll Emergency & Trauma, Haikou 571199, Hainan, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要Hypoxia is a significant global issue affecting the health of organisms. Oxygen homeostasis is critical for mammalian cell survival and cellular activities. Hypoxic stress can lead to cell injury and death, which contributes to many diseases. Sulfane sulfur is involved in crucial roles in physiological processes of maintaining intracellular redox state and ameliorating oxidative damage. Therefore, real-time imaging of changes in sulfane sulfur levels is important for understanding their biofunctions in cells. In this study, we develop a new near-infrared (NIR) fluorescent probe BD-diSeH for imaging of sulfane sulfur changes in cells and in vivo under hypoxic stress. The probe includes two moieties: an NIR azo-BODIPY fluorophore equipped with a strong nucleophilic phenylselenol group (-SeH). The probe is capable of tracing dynamic changes of endogenous sulfane sulfur based on a fast and spontaneous intramolecular cyclization reaction. The probe has been successfully used for imaging sulfane sulfur in 3D-multicellular spheroid and mouse hippocampus under hypoxic stress. The overall levels of sulfane sulfur are affected by the degree and length of hypoxic stress. The results reveal a close relationship between sulfane sulfur and hypoxia in living cells and in vivo, allowing better understanding of physiological and pathological processes involving sulfane sulfur. Moreover, to investigate the effects of environmental hypoxia on aquatic animals, this probe has been applied for sulfane sulfur detection in hypoxic zebrafish.
文章类型Article
资助机构National Nature Science Foundation of China [21775162, 41776110, 21575159]; program of Youth Innovation Promotion Association, CAS [2015170]; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22]; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705]
收录类别SCI
语种英语
关键词[WOS]HYDROGEN POLYSULFIDES; LIVING CELLS; ZEBRAFISH EMBRYOS; CYSTEINE; SULFIDE; H2S; PERSULFIDE; CONSTRUCTION; MODULATION; CHEMISTRY
研究领域[WOS]Materials Science, Biomaterials
WOS记录号WOS:000448340500015
引用统计被引频次：22[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24573
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
3.Hainan Med Univ, Inst Funct Mat & Mol Imaging, Coll Emergency & Trauma, Haikou 571199, Hainan, Peoples R China;
4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
推荐引用方式
GB/T 7714Gao, Min,Wang, Rui,Yu, Fabiao,et al. Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe[J]. JOURNAL OF MATERIALS CHEMISTRY B,2018,6(41):6637-6645.
APAGao, Min,Wang, Rui,Yu, Fabiao,Li, Bowei,&Chen, Lingxin.(2018).Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe.JOURNAL OF MATERIALS CHEMISTRY B,6(41),6637-6645.
MLAGao, Min,et al."Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe".JOURNAL OF MATERIALS CHEMISTRY B 6.41(2018):6637-6645.
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