Chen Jun; Shi Wenjun; Li Wenjun
; Chen Gao; Qin Song发表期刊JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
2018-11
卷号36期号:6 SI页码:2322-2332
关键词Synechocystis sp. PCC 6803genome resequencingnon-motilegenetic background
研究领域Marine & Freshwater Biology; Oceanography
DOI10.1007/s00343-019-7291-1
产权排序[Chen Jun; Shi Wenjun; Li Wenjun; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Chen Jun; Shi Wenjun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen Gao] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Synechocystis sp. PCC 6803 is a model organism widely used in cyanobacterium biology and biotechnology. To know the genetic background of substrains of Synechocystis sp. PCC 6803 is important for further research and application. In this study, we reported the genome sequences of two non-motile wild-type substrains of Synechocystis sp. PCC 6803 using whole genome resequencing. 55/56 putative single nucleotide polymorphisms (SNPs) and 8/9 Indels (insertion and deletion) were identified. Among these, 47 SNPs were found in both the GT-AR and GT-CH strains, and 8 were unique to GT-AR and 9 were unique to GT-CH. All of these variations were annotated in metabolism pathway referred to KEGG database. Meanwhile, the deletion in slr0332 gene was detected in these two strains, which attributed to the non-motile phenotype of them and suggested that the insertion in spkA gene was not essential for non-motile phenotype. These resequencing data provide the genetic background information of these two strains and highlighted the microevolution over decades of laboratory cultivation.
文章类型Article
资助机构National Key Research and Development Program of China [2016YFF0202304]; National Natural Science Foundation of China [41376139]; Science and Technology Program of Yantai City [2016JHZB007]; Natural Science Foundation of Shandong Province, China [ZR2016CM48]
收录类别SCI
语种英语
关键词[WOS]PURIFICATION; MOTILITY; SEQUENCE; PCC-6803; GROWTH
研究领域[WOS]Limnology; Oceanography
WOS记录号WOS:000454629000034
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24570
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
推荐引用方式
GB/T 7714Chen Jun,Shi Wenjun,Li Wenjun,et al. Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(6 SI):2322-2332.
APAChen Jun,Shi Wenjun,Li Wenjun,Chen Gao,&Qin Song.(2018).Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(6 SI),2322-2332.
MLAChen Jun,et al."Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.6 SI(2018):2322-2332.
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